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OfflineSillyputty67

Registered: 10/06/12
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: RogerRabbit]
    #17988569 - 03/21/13 09:03 AM (11 years, 1 month ago)

Ive been doing some research. Granted im not top of my game where this is concerned. Now this is a more reasonable answer. There's why im asking in this forum. I just started to study fungus late last year. This rabbit hole is sooooo deep. Itll take some time.... Mind you im no university student etc.. Just a normal smart ass type, who dosnt believe in imposibillity. I have a very good understanding of science. Biology is kinda a weak point tho, as I didnt find interest when i was a child. I always was working the electrical/technical/physics/astronomy angles. I also have an excellent grasp of inorganic chemistry, and have some elementary skill in organic chem as well. When I got into brewing and Marijuana cultivation, and aquariums, my interest in biology peaked, so here I am. I am a network engineer by trade, and make the impossible happen every day.

I read a lot of science news, and it seems that genetic engineering is getting simpler. Almost simple enough for me to start tackling it.

Use a machine. Sequence an active, and nonactive. Use the computer to compare rna pairs and point out which are present in the active, and not in the nonactive, and then start looking through the remaining metabolic processes, and establish a process of elimination.

Then get the right enzymes to break off, and isolate the exact sequence you need.

This is where my understanding wanes. You give it to yeast and they assemble it automatically. Magically..... Yes more research will be required on my part, but in no way did I say that i wasnt geared to do any... You just liberally lump me into a category with the other lazy brains, and treat me accordingly.


I saw a Ted Talk last year where a brilliant professor re-wrote the dna of an E-coli, and watermarked it with a special code. Methylated the cell, lo and behold it "booted up" and became the worlds first synthetic life. Yeast can apparently assemble dna of any genus or species. Im not sure what gives yeast these seemingly magical properties.


Ive also had previous ideas of isolating the FoxP2 gene, which is only present in humans. It is considered responsible for speech and higher thought processes in humans. If this could somehow be given to a dog, well, Ill let you imagine that scenario.


When I brought this idea here, It was because i respect this community, and value its input. I am a science-type. I understand your point of classification. Yeast is fungus,and the argument was whether or not this fell under an "advanced mycology topic", and in no way did i misclassify. You just circumlocuted by saying yeast dosnt care what you call it. Thats a cop out. This was the first step in trying to make this a reality. It would be a good project for beginning simple engineering. In 10 years kids will be doing this in middle school.

http://blogs.smithsonianmag.com/artscience/2013/02/the-story-of-how-an-artist-created-a-strange-genetic-hybrid-of-himself-and-a-petunia/

:thumbup:

RR your posts are often of a "tag em, bag em, ship em out" mentality. Your angle here is askew. If you read my posts, im trying to get a platform of individuals to contribute to this. Pool money, brain power etc. Because you are the man, your rants, flames, and unconstructive contributions are tolerated. You abuse this position. I think you would be hard pressed finding someone to disagree with this deduction.

If this idea didnt give you a "giggity giggity, i want to contribute to this project." notion and instead gave you a "I want to defer, shut down, and discourage." kind of answer, there is something wrong with your wiring..... This behavior is often referred to as troll like.

:lolsy:

RR I can however thank you for your positive contributions in other posts, regardless if your the mega troll for some reason in mine.:highfive: Lols. Especially when the next comparable post in "advanced mycology" is "PC petri dishes?"......... :rofl:

Frankly i dont give a shit how prestigious a diploma you hoarde and masturbate, or how you look to yourself in the mirror everyday. I did not mean to injure your ego, by posting something out of your ballpark. I get folk with masters degrees around me all the time, and dont have half of the skill or tricks that I have. I work with doctors, and high powered admin staff, who look up to and respect me. I respect others intellects by how they come off, how intuitive they are, how logical their process is, and frankly just how easy to get along with they are. A piece of paper means shit to me. I dont care about the (overpriced society stamp of approval) that certifies that you can absorb, regurgitate, and forget some data. Ill work with anyone who shows initiative, and skill, regardless of the amount of facts the person has absorbed. In todays world of technology, all these processes are relatively super simplified, by the vast processing power of computers, which is my forte. Ive built my own linux distro's from scratch (gentoo stage 1 many times), so complexity is not a stumbling point. Ive built openmosix and beowulf supercomputing platforms. I work with a virtualization environment everyday. So condescention is just an indication of some sort of ego complex. RR just be cool bro. We all have our specialties. :rockon: Yes, I too have an ego. One big enough to not be intimidated by yours. We are all people here, and have a common goal. To change the world in a positive light with science. So hopefully that is something we can agree upon, and is an idea you can get behind. :thumbup:


Check this link, its the ted talk i saw a while ago. It has been my inspiration, and has unlocked a tenacity. :smile:
http://www.ted.com/talks/craig_venter_unveils_synthetic_life.html

Edited by Sillyputty67 (03/21/13 07:24 PM)

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OfflineAlan RockefellerM
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: cli_hlt]
    #18004285 - 03/24/13 03:12 PM (11 years, 1 month ago)

Quote:

cli_hlt said:
Home biotech is bit like home computers were in the mid-late 75's or early 80's. No ordinary people could acquire them and use them.





There is a biotech hacker space here in silicon valley, and another is starting in oakland.

http://biocurious.org

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OfflineSillyputty67

Registered: 10/06/12
Posts: 2,239
Loc: Netherlands
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Alan Rockefeller]
    #18004291 - 03/24/13 03:14 PM (11 years, 1 month ago)

Alan, I read your bio a while ago. Have you sequenced active species already?


--------------------
1) Everything I ever posted or say is a lie.

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InvisibleAmphibolos
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
    #18004316 - 03/24/13 03:20 PM (11 years, 1 month ago)

Also, you have to ask he knows or manipulated a platform using at least the illumina technology for nuclear genome sequencing.


We have one at McGill university, but we will need DNA of intense purity


--------------------


"Homo sum ; humani nihil a me alienum puto"

Edited by Amphibolos (03/24/13 03:21 PM)

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OfflineAlan RockefellerM
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67] * 1
    #18004319 - 03/24/13 03:21 PM (11 years, 1 month ago)

Quote:

malicom said:
Have you sequenced active species already?




Yes.  : )

Here are some:

http://www.ncbi.nlm.nih.gov/nuccore/HE994440.1
http://www.ncbi.nlm.nih.gov/nuccore/HE994454.1
http://mushroomobserver.org/92234

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OfflineSillyputty67

Registered: 10/06/12
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Alan Rockefeller]
    #18004330 - 03/24/13 03:23 PM (11 years, 1 month ago)

:heart:


--------------------
1) Everything I ever posted or say is a lie.

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InvisibleAmphibolos
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Alan Rockefeller]
    #18004341 - 03/24/13 03:25 PM (11 years, 1 month ago)

Its the parts of the genome used for taxonomy.

What we would need is a complete nuclear genome sequencing


Do you have a platform using the illumina sequencing at your university?


--------------------


"Homo sum ; humani nihil a me alienum puto"

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OfflineSillyputty67

Registered: 10/06/12
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Loc: Netherlands
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Amphibolos]
    #18004353 - 03/24/13 03:27 PM (11 years, 1 month ago)

This. He just gave it to us.


1 gatcattatt gaataacttt ggcgtggttg tagctggtcc tctcgggggc atgtgctcgc
      61 ccgtcatctt tatatctcca cctgtgcacc ttttgtagac gttggaactg gataggagag
      121 ggacttgtcc ttcaagttga aggttttttc ggcgctctac gttttcatat accccaaaga
      181 atgtaacaga atgtatctta tggctttatg cctataaact atatacaact ttcagcaacg
      241 gatctcttgg ctctcgcatc gatgaagaac gcagcgaaat gcgataagta atgtgaattg
      301 cagaattcag tgaatcatcg aatctttgaa cgcaccttgc gctccttggt attccgagga
      361 gcatgcctgt ttgagtgtca ttaaattctc aaccttacca gcttttgtta gcttgtgtaa
      421 tggcttggac ttgggggtat tttgccggct tctcttgaga tgtcagctcc ccttaaatgc
      481 attagccggc tgcccgctgt ggaccgtcta ttggtgtgat aattatctac gccgtggacg
      541 tctgctctca atgggttgaa gctgcttcta accgtccgtt tattcggaca gcacataatg
      601 acaatttgac ctcaaatcag gtaggactac ccgctgaact taagcatatc aataagcgga
      661 ggaaaagaaa ctaacaagga ttcccctagt aactgcgagt gaagcgggaa aagctcaaat
      721 ttaaaatctg gcggtctttg gctgtccgag ttgtaatcta gagaagtgtt atcc


adenine, cystosine, cant remember....... I was using a protein folding game called eterna. It is exclusively about solving the riddles of how the acids bond in stable structures. Its actually pretty sweet.


--------------------
1) Everything I ever posted or say is a lie.

Edited by Sillyputty67 (03/24/13 03:30 PM)

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OfflineAlan RockefellerM
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Amphibolos]
    #18004374 - 03/24/13 03:31 PM (11 years, 1 month ago)

Quote:

Amphibolos said:
Its the parts of the genome used for taxonomy.




Yes - Mushrooms have around 40 million base pairs and I chop out just 1000 or so, usually with ITS primers.

Quote:

What we would need is a complete nuclear genome sequencing





That's really expensive.

Quote:

Do you have a platform using the illumina sequencing at your university?




No I use sanger sequencing.

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InvisibleAmphibolos
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Amphibolos]
    #18004437 - 03/24/13 03:46 PM (11 years, 1 month ago)

I just wanted to have an overview of how much it would cost.

Also, you'd have to pay them to make the cDNA library which is another thing :crazy:


--------------------


"Homo sum ; humani nihil a me alienum puto"

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OfflineAlan RockefellerM
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Amphibolos] * 1
    #18004471 - 03/24/13 03:52 PM (11 years, 1 month ago)

Quote:

Amphibolos said:
I just wanted to have an overview of how much it would cost.




The cost is highly variable at the moment.

I saw this on wikipedia:

Quote:

In June 2009, Illumina announced that they were launching their own Personal Full Genome Sequencing Service at a depth of 30Γ— for $48,000 per genome.[58] This is still expensive for widespread consumer use, but the price may decrease substantially over the next few years as they realize economies of scale and given the competition with other companies such as Complete Genomics.[59][60] Jay Flatley, Illumina's President and CEO, stated that "during the next five years, perhaps markedly sooner," the price point for full genome sequencing will fall from $48,000 to under $1,000.[61] Illumina has already signed agreements to supply full genome sequencing services to multiple direct-to-consumer personal genomics companies.




I think the price is still closer to 48,000 than 1000, and I think a lot of places would charge a lot more than 48,000.

The lab I sometimes work in pays $10 per sample in chemicals alone to get 1000 base pairs.  We run 96 samples at a time, so it's not a particularly cheap thing to do.  Sometimes we get distracted talking about mushrooms and mess up, wasting the expensive chems.




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Invisibleteknix
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Alan Rockefeller]
    #18004573 - 03/24/13 04:15 PM (11 years, 1 month ago)

Alan,

Do you work alongside a Bioinformaticist?

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OfflinePussyFart
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
    #18004621 - 03/24/13 04:22 PM (11 years, 1 month ago)

Quote:

malicom said:

1 gatcattatt gaataacttt ggcgtggttg tagctggtcc tctcgggggc atgtgctcgc
      61 ccgtcatctt tatatctcca cctgtgcacc ttttgtagac gttggaactg gataggagag
      121 ggacttgtcc ttcaagttga aggttttttc ggcgctctac gttttcatat accccaaaga
      181 atgtaacaga atgtatctta tggctttatg cctataaact atatacaact ttcagcaacg
      241 gatctcttgg ctctcgcatc gatgaagaac gcagcgaaat gcgataagta atgtgaattg
      301 cagaattcag tgaatcatcg aatctttgaa cgcaccttgc gctccttggt attccgagga
      361 gcatgcctgt ttgagtgtca ttaaattctc aaccttacca gcttttgtta gcttgtgtaa
      421 tggcttggac ttgggggtat tttgccggct tctcttgaga tgtcagctcc ccttaaatgc
      481 attagccggc tgcccgctgt ggaccgtcta ttggtgtgat aattatctac gccgtggacg
      541 tctgctctca atgggttgaa gctgcttcta accgtccgtt tattcggaca gcacataatg
      601 acaatttgac ctcaaatcag gtaggactac ccgctgaact taagcatatc aataagcgga
      661 ggaaaagaaa ctaacaagga ttcccctagt aactgcgagt gaagcgggaa aagctcaaat
      721 ttaaaatctg gcggtctttg gctgtccgag ttgtaatcta gagaagtgtt atcc



:mindblown:

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Invisibleteknix
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: PussyFart]
    #18004651 - 03/24/13 04:28 PM (11 years, 1 month ago)

guanine adenine thyamine cytosine, and uracil in rna.

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OfflineSeahawk1
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
    #18004817 - 03/24/13 04:57 PM (11 years, 1 month ago)

Quote:

malicom said:
Quote:

RogerRabbit said:
Quote:

malicom said:
Its completely reasonable that a plasmid auto replicating sequence could be isoloated from active mushrooms. This sequence could in turn be give to yeast, who will then assemble the sequences into their own.




If it's completely reasonable then do it and tell us how it went.
RR






RR your a smart fucker with a lot of experience, but your angles here are often times hostile, or ultra condescending. If anyone were to give some good input i would hope it were you. Instead you hint that im on my own, and this is not a place to share ideas, only methods.


Yeast are fungus. Hence mycology.

An invention always starts as an idea. I assumed the place for input on this idea was here, and a place like this could make this kind of idea a reality. I guess not.  I guess this is only a place for methods already accomplished, and not bleeding edge science. I guess im the ass for assuming....




Point is, people that know how "easy" this would be have already thought about...it's not like it's a novel idea. If we were all in a room together his tone would be less condescending and more joking...
A. you can't just plop the psilo gene in a yeast and get psilo. Is it even a single gene? etc..
B. Like s/he said, it could be construed as a lead in for someone to do their own research, many of us have done this and thus, not had to make a post.
C. Some of this stuff -IS- easy, just expensive. It's hard to convince people to give you $100k(or more) to maybe be able to make an illegal substance from yeast. What would an increasing [psilo] do to the culture?

I like talking about stuff too; but something like this isn't just a web forum brainstorm project. In chemistry elegance is key, growing shrooms and extracting is probably more elegant than a yeast method would be. JMO

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OfflineSillyputty67

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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Seahawk1]
    #18004852 - 03/24/13 05:03 PM (11 years, 1 month ago)

:thumbup: fair enough. I just say we make it so big pharma dosnt get it first.

Quote:

Seahawk1 said:
What would an increasing [psilo] do to the culture?







Make them trip the fuck out or turn them blue. lolz


Blue yeast would change the world. There would no longer be a need for 25i or other synthetics. Other caveats would be what if this yeast escaped? Next thing you know all fruit along the eastern seaboard is now mildly psychedelic. gigity gigity.... Imagine the most pristine summer day, eating a fresh apple with a bluish hue. lol


--------------------
1) Everything I ever posted or say is a lie.

Edited by Sillyputty67 (03/24/13 05:22 PM)

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OfflineRogerRabbitM
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Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
    #18005531 - 03/24/13 05:03 PM (11 years, 1 month ago)

This thread has been closed.

Reason:
This is advanced mycology, not a drug manufacturing forum.  May I suggest ODD or PE.

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