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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Frank's tub-in-tub incubator 2
#17954773 - 03/14/13 12:12 PM (10 years, 10 months ago) |
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Quote:
Citric said:
Quote:
badman said: 7 days from MS impressive.
I went from spore to harvest in 21 =) I'll never not incubate, don't know why people aren't.
So I picked up a few things to get my incubator set up again (my last aqua heater took a crap) and I figured I would share.
I know, half of you are going to say that incubation is outdated, that it increases contam risk, etc.
Well, too bad. 
I keep my temps around 80 for my grain masters, which I find to be perfect and speeds colonization up significantly.
There is no added contam risk to properly prepared and sterilized jars. Endospores that survive the PC cycle take a long time to recover and at 80F, your jars should colonize within 5-14 days, depending on type of inoculation and how much you like to shake the jars while they colonize. And if you have any live mold spores or bacteria in that jar that you think your myc will "outrun" at lower temperatures, then you've already lost the battle.
The design is pretty much just Magash's TiT incubator.
I use two 35gallon opaque plastic tubs, a 50w aquarium heater, 4 tall half-pint jars, and an air stone and air pump. Total cost for me to buy locally was about $50 US. I put the heater on the bottom of the first tub along with the air stone for more even distribution of heat, which i've found to be very important.
The heater is set at 80F.
The second tub sits inside the first. I used tall half-pint jars (submerged) to keep the tub from resting directly on the heater.
I used zip-ties to strap the second tub down to the first. It will definitely try to float if you do not.
Then I fill the water level about halfway up the sides of the second tub. I also add about 1/4 gallon of bleach to every 5 gallons of water or so.
And that's all there is to it 
Optionally you can add a wire shelf cut to fit for an extra bit of space 

Here it is (obviously the lid is on when it's not being messed with, I was just lazy with pictures):

This keeps temps in my cold basement and in my fruiting room as well.
I urge the skeptics to at least give incubation at proper temperatures a chance, you will be surprised at the result 

Edited by FrankHorrigan (04/01/13 03:11 PM)
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 24 days
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its more so to beginners in growing mushroom that we try and stear them away from incubators.
But if you have experience and have your incubator dialed in, theres no reason why they wont get thigns done quicker.
Its better for a spomeone beginning to just be patient and wait the little extra time, rather than having things go wrong and get discouraged.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: My tub-in-tub incubator [Re: veda_sticks]
#17954814 - 03/14/13 12:22 PM (10 years, 10 months ago) |
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Quote:
veda_sticks said: its more so to beginners in growing mushroom that we try and stear them away from incubators.
But if you have experience and have your incubator dialed in, theres no reason why they wont get thigns done quicker.
Its better for a spomeone beginning to just be patient and wait the little extra time, rather than having things go wrong and get discouraged.
I hear that. It's just that most people never get out of that misconception, so hopefully this will help folks be less scared of a little warmth
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
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i have too many jars for a tit 
Edited by twistedty (03/14/13 01:10 PM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 16 days, 12 hours
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Re: My tub-in-tub incubator [Re: twistedty]
#17955003 - 03/14/13 01:08 PM (10 years, 10 months ago) |
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Quote:
twistedty said: i have too many jars for a tit
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: My tub-in-tub incubator [Re: twistedty]
#17955005 - 03/14/13 01:09 PM (10 years, 10 months ago) |
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Quote:
twistedty said: i have too many jars for a tit 
Me too  I just use it for my grain masters and petri dishes. Cuts the colonizing time almost in half. And I can fit almost 60 jars in that TiT. I usually have around 200 spawn jars that get g2g'd into for whatever species I'm working on, they colonize so quick i just keep em at 74F in my grow room
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
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i used to mess with incubators im not knocking them. but i can barely keep up as it is
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
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Re: My tub-in-tub incubator [Re: twistedty]
#17955015 - 03/14/13 01:12 PM (10 years, 10 months ago) |
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i live in my kitchen :/ practically
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: My tub-in-tub incubator [Re: twistedty]
#17955019 - 03/14/13 01:13 PM (10 years, 10 months ago) |
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I hear ya. You should try doing 24 tubs at a time. That's work, son
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
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Quote:
FrankHorrigan said: I hear ya. You should try doing 24 tubs at a time. That's work, son 
im trying lol! i have more jars than cabinets . im aiming for 10ish at a time.
just slanted a few isolates for my first time
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: My tub-in-tub incubator [Re: twistedty]
#17955032 - 03/14/13 01:16 PM (10 years, 10 months ago) |
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Alright, this will not turn into a "Biggest grow" thread
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Zarotti
Stranger

Registered: 05/12/12
Posts: 314
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I can confirm FH
My jars took over 4 weeks when the temps were at around 65F
Since I wrapped them in a heating blanket - they take about 10 days at 80F...
Edited by Zarotti (03/15/13 03:34 AM)
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first time expert
practice makes perfect



Registered: 02/19/13
Posts: 1,246
Loc: top of mount washington
Last seen: 6 years, 4 months
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Re: My tub-in-tub incubator [Re: Zarotti]
#17955226 - 03/14/13 02:01 PM (10 years, 10 months ago) |
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I just bought a tit incubator and after I got it I heard a bunch of people say that it was a bag idea and I would be better off to colonize in room temp. I can say that after 2 weeks of using the tit, It seams to work good for me. I leave the cover off and it stays a consistent 76-78 degrees. it also puts my humidity and temp in my martha up without running a separate heater or humidifier, works good!! I did have to clean the water once but it only took about 10 min, no big deal
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Fungal growth
Lootinint



Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
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so, i know fruiting at higher temps results in (ime) hollow stipes, which always pissed me off. when you colonize at higher temps, is there an impact on fruiting? what temps do you generally fruit at? might be a dumb question, but its mine.
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Doc_D
Indifferent


Registered: 11/13/12
Posts: 350
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Nice work again Frank.
Whilst the air-pump usage may seem obvious to most I think as this is a ‘TEK’ then perhaps the reasoning, placement & rough cost of the air pump should be included?
-------------------- A high-powered mutant of some kind never even considered for mass production. Too weird to live, and too rare to die.
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Citric


Registered: 03/19/04
Posts: 4,490
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Re: My tub-in-tub incubator [Re: veda_sticks] 1
#17956379 - 03/14/13 06:06 PM (10 years, 10 months ago) |
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Quote:
veda_sticks said: its more so to beginners in growing mushroom that we try and stear them away from incubators.
But if you have experience and have your incubator dialed in, theres no reason why they wont get thigns done quicker.
Its better for a spomeone beginning to just be patient and wait the little extra time, rather than having things go wrong and get discouraged.
This.
This is why incubators were looked down upon. Many newbies struggle with proper sterilization as is.
If you are not new, or can afford a good heater(Pro heat serious works awesome) then incubate.
Frank. Now that you have these set up. Could you do a test for me that I never got around to doing.
One big tub for your heater. Place two smaller tubs inside. One clear, one not. Use an isolate. Use small 1/2 pint jars. Don't shake to avoid and chance of randomization. I got money on the dark tub.
-------------------- Self Healing lid tek ** Update 10.17.17 ** Mini casing pictures: Pins to harvest Cup O' Shrooms Magash: I noticed my contams were in the shape of fingers Hyphae: Yes "Loss of moisture from the substrate" is not a casing trigger. My final Grow!
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Intelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
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Re: My tub-in-tub incubator [Re: Citric]
#17956450 - 03/14/13 06:17 PM (10 years, 10 months ago) |
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for Citrics experiment idea.
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HypnotoadCroaked
Retired, but will check MSGs


Registered: 01/05/13
Posts: 1,168
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Mine is a pair of sterilite tubs (The water one is 24qt, and the other is slightly shorter in height...maby 16 qt) inside a larger 72 qt grey tub. The aquarium heater is inside the clear 24 qt. I use a 5-10 gallon rated heater that is automatically set at 78-80 degrees. It don't hold alot of quart jars, but it does make a huge difference in colonization times. I have not been concerned with what nay-sayers have said about TiTs. I love TiTs. I love tits too.
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thelanzii

Registered: 11/13/12
Posts: 5,434
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I used an incubator for a weekish was not too big of a fan. I also did not put an air stone in it. Got this beautiful piece of equipment the other day Couldn't recommend it more!
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HypnotoadCroaked
Retired, but will check MSGs


Registered: 01/05/13
Posts: 1,168
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Re: My tub-in-tub incubator [Re: thelanzii]
#17957958 - 03/14/13 10:52 PM (10 years, 10 months ago) |
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Quote:
Nemmies said: I also did not put an air stone in it. Got this beautiful piece of equipment the other day
Thats good. I don't use an air stone in my TiT either. Heating a room is always better than heating a tub of fluid in most cases. I however CANNOT due to limitations of the design of my home get my office up over 70 this time of year. Id love to have a space that I can jack up to the high 70s....but at this time of year, Id love to have a space I can jack up to the low 70s lol.
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