Home | Community | Message Board


Shroom Supply
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar, Scales

Jump to first unread post. Pages: 1
Invisiblevampirism
Stranger
Male User Gallery

Registered: 03/14/04
Posts: 8,120
gar.. dog food agar questions ( silly obstruction..)
    #1795285 - 08/09/03 02:42 PM (13 years, 3 months ago)

Ok, i was planning on using rush wayne's peroxide method with dfa
but i have run into a very simple problem: i have no scale

does anyone know of an average density for dry agar agar?
ill get the volume of 20g dry dog food just by dividing total weight by volume and so forth.. but i dont know the weight of my agar at all

and also

using the peroxide method, how important is it to have sterile h2o2? Is there some way I could check the sterility? I have some mildly old peroxide, but is it ok to use?



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSev
Astropath
Registered: 06/06/03
Posts: 1,426
Loc: NY
Last seen: 2 years, 1 month
Re: gar.. dog food agar questions ( silly obstruction..) [Re: ]
    #1795688 - 08/09/03 05:07 PM (13 years, 3 months ago)

You don't need sterile H2O2 -- after all, it's there to kill things.  :wink:

So long as the peroxide was stored in a cool, dark place, it should be fine for a year or two.


--------------------
"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

All of my posts are full of fiction and blatant lies.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblevampirism
Stranger
Male User Gallery

Registered: 03/14/04
Posts: 8,120
Re: gar.. dog food agar questions ( silly obstruction..) [Re: Sev]
    #1795912 - 08/09/03 06:23 PM (13 years, 3 months ago)

aye, but i read that sometimes it has mold particulates floating around- sort of establish mold mycelium which would contam my plates


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: gar.. dog food agar questions ( silly obstruction..) [Re: ]
    #1797029 - 08/10/03 02:02 AM (13 years, 3 months ago)

Not much will live in hydrogen peroxide. The volume of the agar depends on the specific agar, but you could always do it by the amount of water displaced.... You'd have to know the specific density, however, but this may be on the bottle.

A drop scale should work, or a nickel weighs 5 grams. It's not really sensitive, but you want to be close.

BTW -- dog food agar contaminates really easily, IME. I don't use peroxide, however.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblevampirism
Stranger
Male User Gallery

Registered: 03/14/04
Posts: 8,120
Re: gar.. dog food agar questions ( silly obstruction..) [Re: micro]
    #1797674 - 08/10/03 10:45 AM (13 years, 3 months ago)

interesting.. i've noticed some places that recommend it, and have used it successfully for morels and other interesting fellas.. im going to use it on pl. djamor ( most likely), so i shouldn't run into contam problems, esp with peroxide


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: gar.. dog food agar questions ( silly obstruction..) [Re: ]
    #1798172 - 08/10/03 02:58 PM (13 years, 3 months ago)

Doubtful you'll have problems with peroxide. Like I said -- I don't use it, so I have to worry about what I use more.

I will use gentamycin sometimes, though.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: Agar, Scales

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Agar questions Mych 5,222 8 06/23/05 07:04 PM
by drugsaregood
* Agar Questions Cyber 1,221 6 09/26/07 08:54 PM
by RogerRabbit
* silly agar questions - new trials beginning felixhigh 1,716 11 03/27/02 07:48 PM
by ParticleMan
* agar question
( 1 2 all )
HongKongChewy 2,980 29 08/24/02 10:07 AM
by TripsAreForKids
* A few Agar Questions
( 1 2 all )
drwatson 3,736 25 12/01/07 07:44 PM
by fastfred
* Quick agar question Fr0ggy 1,151 11 05/03/03 08:10 AM
by Fr0ggy
* Agar Question... dice68 1,097 6 11/07/01 03:21 PM
by iudexk
* agar questions WhereAbouts 1,284 11 06/08/01 04:47 AM
by Una

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
1,333 topic views. 0 members, 1 guests and 1 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Phytoextractum
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.002 seconds on 14 queries.