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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Quick report on reusing contaminated spawn jars 3
#17951157 - 03/13/13 08:36 PM (10 years, 10 months ago) |
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Wanted to make a quick report on this while I have it in my mind. This is not a tek. I've seen it debated, and I've seen experienced opinions shared on the subject, but what I had never seen was any actual experimentation. It has been argued as a lost cause, and to toss it. The idea of pressure cooking the contams and inoculating the jars again was also used multiple times. Here is some actual experimentation.
This is a pic of 7 heavily contaminated spawn jars, they were contaminated with everything from bacteria, to Dactylium, to multiple Aspergillus sp. They were the result of using wild-found spores from multiple species, and some contaminated P.cubensis.

Pressure cooked them for 60 minutes @ 15 PSI, here is a pic of the same jars(and one extra), 8 days after clean MS inoculation:

Some minor details, I did this with 11 jars total over the course of 3 weeks, and all of the jars that were PC'ed and re-inoculated have a 100% success rate thus far. I did shake the moldy jars like crazy before PC'ing them. Keep in mind you want to PC the contams at first sight instead of allowing them to get out of hand, because they can cause dramatic changes in the pH, thus causing issues with the second inoculation/colonization. The jars which would have been thrown away are performing with no problems, although they would have likely performed better before the second PC'ing, it's hard to complain seeing the results in person.
A quick rundown of the entire process:
Prepared spawn jars. Pressure cooked spawn jars for 100mins @ 15psi. Inoculated spawn jars. 7 jars contaminated days later. Pressure cooked contaminated spawn jars for 60mins @ 15PSI. Inoculated previously-contaminated-double-pressure-cooked spawn jars. Healthy mycelium, no contamination, grains not too dry, good colonization speeds maintained.
Closing statement; do not open contaminated jars before pressure cooking. The double PC'ed jars will likely not perform as well as the single PC'ed uncontaminated jars. Do not attempt to inject water into grain jars before second pressure cooking. All molds will be killed completely with 60mins of 15PSI.
-------------------- ¯\_(ツ)_/¯
Edited by Tmethyl (03/13/13 10:09 PM)
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Mooby
Stranger



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Re: Quick report on reusing contaminated spawn jars [Re: Tmethyl]
#17951289 - 03/13/13 08:52 PM (10 years, 10 months ago) |
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This is just awesome! It makes total sense that this would work. I love reading about your experiments; they've saved from some stupid mistakes, like throwing away materials. I'm totally just re-PCing my only bad jar ever with the next round of new ones. This could not be more timely for me.
-------------------- Subvert the dominant paradigm; pee in the sink!
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fungal_alchemist
Ghetto-Engineer



Registered: 03/19/12
Posts: 296
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Re: Quick report on reusing contaminated spawn jars [Re: Mooby]
#17951434 - 03/13/13 09:08 PM (10 years, 10 months ago) |
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As always a great contribution to the community thanx for documenting another great experiment
-------------------- Grocery Store Agar Tek damion5050's coir Tek A. augustus grow "I see the mycelium as the Earth's natural Internet, a consciousness with which we might be able to communicate... Because these externalized neurological nets sense any impression upon them, from footsteps to falling tree branches, they could relay enormous amounts of data regarding the movements of all organisms through the landscape." Paul Stamets
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HypnotoadCroaked
Retired, but will check MSGs


Registered: 01/05/13
Posts: 1,168
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Re: Quick report on reusing contaminated spawn jars [Re: Mooby]
#17951452 - 03/13/13 09:10 PM (10 years, 10 months ago) |
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I have already re-PC'd contaminated jars and re-inoculated. I do not post results here because there are flocks of nay-sayers that seem to be drawn to pointing out your future failures, with no proof or supporting evidence. I currently have 2 jars that had mold that are doing splendid after an hour and a half at 250 degree steam. I have one more from a past batch of experiments that picked up the trich, and when I free up some more lids, it will be getting the same treatment.
I am glad one of the more trusted members has forged forward with these results. I really think that If I had even posted what I had done over the last few weeks with the exact same situation, I would have been flamed for "obvious illiteracy" when it comes to "Its contaminated THROW IT OUT".
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newera
Stranger


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Re: Quick report on reusing contaminated spawn jars [Re: HypnotoadCroaked]
#17951466 - 03/13/13 09:12 PM (10 years, 10 months ago) |
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how many times can i put grains in the pc? i had to do 3 runs do to excess is that over kill?
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Groc60
Scientist



Registered: 08/05/12
Posts: 114
Loc: Mars
Last seen: 6 years, 1 month
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Re: Quick report on reusing contaminated spawn jars [Re: Tmethyl]
#17951494 - 03/13/13 09:16 PM (10 years, 10 months ago) |
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Nice! learn something new everyday! was that 'plus one jar' contaminated? If it wasn't you should post a comparison view.
-------------------- "And they say that I look higher in person" My Easy Coffee Can FC
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SpitballJedi
Ancient Astronaut


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Quick report on reusing contaminated spawn jars [Re: Mooby]
#17951505 - 03/13/13 09:17 PM (10 years, 10 months ago) |
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Great start to an excellent collection of data. Defiantly worth noting.
As I know you are wise, I'm sure you are fully prepared for the onslaught nay-sayers as well as the bandwagoneers.
May I suggest that you mention that this is not intended to be a tek for everyone to jump on? You know how people are.
I am curious to see how the run plays out, i.e. yields, size, contamination later.
We can always count on you to not take peoples word and conduct your own experiments. You are not just a cultivator, but a mycologist.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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newera
Stranger


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Re: Quick report on reusing contaminated spawn jars [Re: SpitballJedi]
#17951537 - 03/13/13 09:21 PM (10 years, 10 months ago) |
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how do you spot a fresh contam? or do the old ones vanish?
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Groc60
Scientist



Registered: 08/05/12
Posts: 114
Loc: Mars
Last seen: 6 years, 1 month
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Re: Quick report on reusing contaminated spawn jars [Re: newera]
#17951552 - 03/13/13 09:24 PM (10 years, 10 months ago) |
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Quote:
newera said: how do you spot a fresh contam? or do the old ones vanish?
If something looks wrong or smells wrong. Usually contams don't magically vanish. EDIT: Typo
-------------------- "And they say that I look higher in person" My Easy Coffee Can FC
Edited by Groc60 (03/13/13 09:24 PM)
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SpitballJedi
Ancient Astronaut


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Quick report on reusing contaminated spawn jars [Re: Groc60]
#17951630 - 03/13/13 09:38 PM (10 years, 10 months ago) |
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I think what he's asking is "if you do get contaminated after the second PC and inoc, how do you know if its a brand new contam from the inoc or if it's a contam from earlier."
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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cherry_darling
i smell horseshit!



Registered: 12/08/12
Posts: 589
Loc: Planet Terror (ohio)
Last seen: 10 years, 6 months
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Re: Quick report on reusing contaminated spawn jars [Re: Groc60]
#17951651 - 03/13/13 09:41 PM (10 years, 10 months ago) |
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Sorry if these are tard questions, but here goes:
1. After PC'ing, the mold mycelium is dead, but still there right? So the shroom myc will "eat" the old dead mold mycelium? If so, that's pretty cool..
2. You didn't (obviously, going by the pics!) experience moisture loss after PC'ing the grain twice? I'd have figured it would dry it out a little too much.....I wonder how many PC cycles a jar of grain could take before getting too dry, or would that not even happen?
I love seeing your experiments, you are indeed a man of action!
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: Quick report on reusing contaminated spawn jars [Re: cherry_darling]
#17951743 - 03/13/13 09:56 PM (10 years, 10 months ago) |
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Quote:
fungal_alchemist said: As always a great contribution to the community thanx for documenting another great experiment
Thank you, although this one was poorly documented I think.
Quote:
Whippy said: I have already re-PC'd contaminated jars and re-inoculated. I do not post results here because there are flocks of nay-sayers that seem to be drawn to pointing out your future failures, with no proof or supporting evidence. I currently have 2 jars that had mold that are doing splendid after an hour and a half at 250 degree steam. I have one more from a past batch of experiments that picked up the trich, and when I free up some more lids, it will be getting the same treatment.
I am glad one of the more trusted members has forged forward with these results. I really think that If I had even posted what I had done over the last few weeks with the exact same situation, I would have been flamed for "obvious illiteracy" when it comes to "Its contaminated THROW IT OUT".
This is a common theme at the Shroomery. A lot of good cultivators moved to other websites due to this. Next time though, just post anyways. Not everyone is on such a knowledge plateau. You're ideas and experiments will receive good and bad feedback no matter what they are, this is a good thing. Thank you for your honesty here, it is certainly a logical solution to save on time and wasted materials.
Quote:
Groc60 said: Nice! learn something new everyday! was that 'plus one jar' contaminated? If it wasn't you should post a comparison view.
The 8th jar was done before the 7 in the first pic, my PC only holds 7 quarts at a time. The 8th jar is not P.cubensis, it's Gymnopilus. The macroscopic colonization patterns look vastly different, which is why I didn't include it in the shot.
Quote:
SpitballJedi said: Great start to an excellent collection of data. Defiantly worth noting. As I know you are wise, I'm sure you are fully prepared for the onslaught nay-sayers as well as the bandwagoneers. I am curious to see how the run plays out, i.e. yields, size, contamination later.
I will continue to experiment with this and post the results of these jars as the develop fruiting cultures. Being that they are MS, won't help determine much in the way of yields when comparing previously contaminated jars to clean-first-time jars, but it's worth documenting. Also, yes, I am prepared for a shit storm, but I have a feeling it will be anticlimactic and uneventful due to a lack of evidence proving this does not work. I look forward to it, however.

Quote:
SpitballJedi said: May I suggest that you mention that this is not intended to be a tek for everyone to jump on? You know how people are. We can always count on you to not take peoples word and conduct your own experiments. You are not just a cultivator, but a mycologist.
I didn't label it as a tek, for this reason. I used 'report'. Thank you for mentioning this. This report is one of many to come, from me and hopefuly from others. Thank you for your kind words as well, it is sincerely heartfelt.Quote:
cherry_darling said: Sorry if these are tard questions, but here goes:
1. After PC'ing, the mold mycelium is dead, but still there right? So the shroom myc will "eat" the old dead mold mycelium? If so, that's pretty cool..
2. You didn't (obviously, going by the pics!) experience moisture loss after PC'ing the grain twice? I'd have figured it would dry it out a little too much.....I wonder how many PC cycles a jar of grain could take before getting too dry, or would that not even happen?
I love seeing your experiments, you are indeed a man of action! 
1) Live mycelium will consume dead mycelium, as well as the nutrients the dead mycelium freed from the substrate whilst it was still living. Dead mycelium simply becomes part of the substrate, and is treated as so. But keep in mind, dead mycelium is different than spent substrate mycelium.
2)There was some moisture loss, it was marginal though. I wouldn't PC more than twice, but I'm sure 3x would still work. Just keep in mind you want to PC the contams at first sight instead of allowing them to get out of hand, because they can cause dramatic changes in the pH, thus causing issues with the second inoculation/colonization.
-------------------- ¯\_(ツ)_/¯
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op2kal



Registered: 08/31/12
Posts: 1,167
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Re: Quick report on reusing contaminated spawn jars [Re: Tmethyl]
#17951944 - 03/13/13 10:30 PM (10 years, 10 months ago) |
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Cool man just figured i'd chime in a little so i could follow along as to the progress of this experiment.
Your a good guy timethyl
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Brain Fart
Mushroom Nerd



Registered: 12/19/07
Posts: 2,538
Loc: Your Mom
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Re: Quick report on reusing contaminated spawn jars [Re: op2kal]
#17952140 - 03/13/13 11:14 PM (10 years, 10 months ago) |
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Quote:
Whippy said: I have already re-PC'd contaminated jars and re-inoculated. I do not post results here because there are flocks of nay-sayers that seem to be drawn to pointing out your future failures, with no proof or supporting evidence. I currently have 2 jars that had mold that are doing splendid after an hour and a half at 250 degree steam. I have one more from a past batch of experiments that picked up the trich, and when I free up some more lids, it will be getting the same treatment.
I am glad one of the more trusted members has forged forward with these results. I really think that If I had even posted what I had done over the last few weeks with the exact same situation, I would have been flamed for "obvious illiteracy" when it comes to "Its contaminated THROW IT OUT".
Quote:
Timethyl said:
This is a common theme at the Shroomery. A lot of good cultivators moved to other websites due to this. Next time though, just post anyways. Not everyone is on such a knowledge plateau. You're ideas and experiments will receive good and bad feedback no matter what they are, this is a good thing. Thank you for your honesty here, it is certainly a logical solution to save on time and wasted materials.
I'm sorry but no website is cooler then the shroomery. even if people can be butt plugs sometimes here. myself included
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Badaboom
Traveler



Registered: 02/12/13
Posts: 657
Loc: Central States
Last seen: 27 days, 20 hours
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Re: Quick report on reusing contaminated spawn jars [Re: Brain Fart]
#17952248 - 03/13/13 11:37 PM (10 years, 10 months ago) |
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How are you not a TC...?
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thelanzii

Registered: 11/13/12
Posts: 5,434
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Re: Quick report on reusing contaminated spawn jars [Re: Badaboom]
#17952529 - 03/14/13 12:29 AM (10 years, 10 months ago) |
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Gonna give it a shot, nothing to lose. Would reinoculating jars that failed to colonize fall under this same concept?
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: Quick report on reusing contaminated spawn jars [Re: thelanzii]
#17953309 - 03/14/13 04:47 AM (10 years, 10 months ago) |
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Yes Nemmies but a jar failing to colonize is likely a GE issue. Could be bacteria too though.
-------------------- ¯\_(ツ)_/¯
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Pirax
Self Employed Pharmacist

Registered: 11/26/12
Posts: 388
Loc: Boregon
Last seen: 7 years, 6 months
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Re: Quick report on reusing contaminated spawn jars [Re: Tmethyl]
#17953453 - 03/14/13 05:38 AM (10 years, 10 months ago) |
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This is bad mother-fucking ass. Does that mean I can Re-PC my contaminated agar halfpint dishes and reuse them ? the asian foor store is quite a trip away
Also do you think this method would work for pf cakes as well?
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
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Re: Quick report on reusing contaminated spawn jars [Re: Pirax]
#17953532 - 03/14/13 06:12 AM (10 years, 10 months ago) |
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With agar i highly doubt it, with cakes its possible. Basically both methods will require testing before anyone can say with any certainty.
-------------------- ¯\_(ツ)_/¯
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Quick report on reusing contaminated spawn jars [Re: Tmethyl]
#17954068 - 03/14/13 09:08 AM (10 years, 10 months ago) |
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Great experiment, I have been tempted to try this, but have always been too pussy to go for it. Though it seemed reasonable enough in theory.
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HypnotoadCroaked
Retired, but will check MSGs


Registered: 01/05/13
Posts: 1,168
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Re: Quick report on reusing contaminated spawn jars [Re: Brain Fart]
#17955472 - 03/14/13 03:13 PM (10 years, 10 months ago) |
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Quote:
Brain Fart said: I'm sorry but no website is cooler then the shroomery. even if people can be butt plugs
Ive already "ignored" one of the usual Parrots. There are times that I find that repeatedly regurgitating someone else's observations and studies abhorrent.
But I digress. You are TOTALLY correct Brain Fart. This is a spectacular site, with great resources and a stellar group of "volunteers"....We are all volunteers here btw.
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Jenooba



Registered: 09/27/12
Posts: 23
Loc: Hilo, Hawaii
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Re: Quick report on reusing contaminated spawn jars [Re: HypnotoadCroaked]
#17955800 - 03/14/13 04:23 PM (10 years, 10 months ago) |
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Looking great, Tmethyl. I haven't had any issues with contaminants yet, but I'll have to consider this as an option when I do. As has been said, please keep us updated on the results! 
You rock, man.
-------------------- Life is all about perspective; don't get too caught up in the one you're experiencing right now.
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hyphal
Enthusiast



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Loc: Planet Earth
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Re: Quick report on reusing contaminated spawn jars [Re: HypnotoadCroaked]
#17955853 - 03/14/13 04:35 PM (10 years, 10 months ago) |
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Quote:
Whippy said:
Quote:
Brain Fart said: I'm sorry but no website is cooler then the shroomery. even if people can be butt plugs
Ive already "ignored" one of the usual Parrots. There are times that I find that repeatedly regurgitating someone else's observations and studies abhorrent.
But I digress. You are TOTALLY correct Brain Fart. This is a spectacular site, with great resources and a stellar group of "volunteers"....We are all volunteers here btw.
Agreed, Whippy. We have a saying in horticulture that "plants don't read books or listen to expert horticulturists." I think it apropos here, as well. The myc just wants to run...
-------------------- Surfing the Wood Wide Web
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MushGrooveFarms
Extra-Terrestrial


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Re: Quick report on reusing contaminated spawn jars [Re: hyphal]
#17956204 - 03/14/13 05:42 PM (10 years, 10 months ago) |
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Funny, I was wondering if this would be successful as I logged on. Perfect timing. Five jars in the PC now. My print was contammed with trich, so I won't be re-innoculating with that. But it seems I will be able to save my grain! Huzzah! Time to get into agar for those dirty spores.
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shroom-a-zoom
Stranger in a strange land


Registered: 02/26/13
Posts: 414
Loc: Fungiland
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Re: Quick report on reusing contaminated spawn jars [Re: MushGrooveFarms]
#18008198 - 03/25/13 11:51 AM (10 years, 10 months ago) |
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I just so happen to have 3 jars with some green in them. I can give this a try if I have the time in the next day or two. If not, they are going outside. I will post either way. Peace SAZ
-------------------- How it should & shouldn't look - NEW CULTIVATORS GUIDE
Lets get rid of the SWIM shit, This also goes for my dog, someones pet ferret, and any other way of saying you are not really you. Its bullshit and we all know it. Man up and admit you are the SWIM.
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shroom-a-zoom
Stranger in a strange land


Registered: 02/26/13
Posts: 414
Loc: Fungiland
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Re: Quick report on reusing contaminated spawn jars [Re: shroom-a-zoom]
#18045356 - 04/02/13 07:53 AM (10 years, 9 months ago) |
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Quote:
shroom-a-zoom said: I just so happen to have 3 jars with some green in them. I can give this a try if I have the time in the next day or two. If not, they are going outside. I will post either way. Peace SAZ
I have not had time since this happened so the jars still sit as is and nasty. I dont think I am going to be trying to reuse these.
-------------------- How it should & shouldn't look - NEW CULTIVATORS GUIDE
Lets get rid of the SWIM shit, This also goes for my dog, someones pet ferret, and any other way of saying you are not really you. Its bullshit and we all know it. Man up and admit you are the SWIM.
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mushmybush
Stranger
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Re: Quick report on reusing contaminated spawn jars [Re: cherry_darling]
#18413396 - 06/13/13 12:37 PM (10 years, 7 months ago) |
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What happens to the bacteria in a PC when the temp gets high enough to a point where weak molecular bonds such as hydrogen bonding and ionic bonds break to form covalent bonds in the wrong places. This is denaturation in an organism. Structure and function are highly correlated together so when you break the structure of the bacteria (killing it) it is unable to function (live.)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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Re: Quick report on reusing contaminated spawn jars [Re: mushmybush]
#18413417 - 06/13/13 12:41 PM (10 years, 7 months ago) |
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not all bacteria/molds will be killed in a pc, what we call sterile isn't really even close, just gives us a jumpstart on competeing organisms long enough for our mycelium to get a foot hold
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ConcreteWaves



Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 1 year, 2 months
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Re: Quick report on reusing contaminated spawn jars [Re: cronicr]
#18413653 - 06/13/13 01:32 PM (10 years, 7 months ago) |
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Ok so - >.> I've been wondering this myself Tmethyl everytime i get a contaminated jar. So is this entire method stating...ur PCing the jar WITH the mold inside of it >.> taking it out, and just G2G xfering more culture into it, or knocking it up w/ syringe?
If this is the case....

where the fuck does the mold go >.> Gas exchange through the polyfil? Shouldnt u change ur polyfil then afterwords....
In my mind....even water evaporates into a gas. A full on mold culture wont just disappear, it goes somewhere >.>
Or are u talking about PCing the moldy jar, taking it out, cleaning it, and having a fresh jar for new grains and culture?
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Quick report on reusing contaminated spawn jars [Re: ConcreteWaves]
#18413691 - 06/13/13 01:42 PM (10 years, 7 months ago) |
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He leaves the moldy grains in the jar and reinoculates the jar after re-sterilizing.
The mold and spores get killed well below 15psi.
The healthy culture just colonizes right over it, it's all dead organic matter to the myc.
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ConcreteWaves



Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 1 year, 2 months
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Re: Quick report on reusing contaminated spawn jars [Re: FrankHorrigan]
#18413727 - 06/13/13 01:50 PM (10 years, 7 months ago) |
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Quote:
FrankHorrigan said: He leaves the moldy grains in the jar and reinoculates the jar after re-sterilizing.
The mold and spores get killed well below 15psi.
The healthy culture just colonizes right over it, it's all dead organic matter to the myc.
But any MYC that was in the jar gets destroyed aswell, that's why we add new culture to the jar, correct? At those temps, nothing survives aye?
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Quick report on reusing contaminated spawn jars [Re: ConcreteWaves]
#18413731 - 06/13/13 01:51 PM (10 years, 7 months ago) |
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Yep
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tbagtag
Boomer Barron

Registered: 01/16/13
Posts: 1,432
Loc: Amsterdam
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Re: Quick report on reusing contaminated spawn jars [Re: FrankHorrigan]
#18413841 - 06/13/13 02:14 PM (10 years, 7 months ago) |
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My PB thread was based on Tim's suggestion to re-pc. I'm on my 3rd flush of some super potent cubes and my yield was the highest ever. The re-pc doesn't have any adverse effects so far.
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: Quick report on reusing contaminated spawn jars [Re: tbagtag]
#18413916 - 06/13/13 02:27 PM (10 years, 7 months ago) |
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Thanks for the help Frank.
Well done tbag, I still haven't experienced any down side to this practice either. The warnings in the OP are just logical speculation, they likely lose some moisture so they are likely less optimal than the first round.
-------------------- ¯\_(ツ)_/¯
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ConcreteWaves



Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 1 year, 2 months
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Re: Quick report on reusing contaminated spawn jars [Re: FrankHorrigan]
#18414083 - 06/13/13 03:05 PM (10 years, 7 months ago) |
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Quote:
Tmethyl said: Thanks for the help Frank.
Well done tbag, I still haven't experienced any down side to this practice either. The warnings in the OP are just logical speculation, they likely lose some moisture so they are likely less optimal than the first round.
Quote:
FrankHorrigan said: Yep 
would u 2 suggest only doing this with jars that have observed mold SPOTS coming in, not a jar that's been almost overrun with it? I mean, i guess i can answer my ownquestion aye, thats common sense. But you think it would work on a jar that's got alot of colonized mold?
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: Quick report on reusing contaminated spawn jars [Re: ConcreteWaves]
#18414124 - 06/13/13 03:13 PM (10 years, 7 months ago) |
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The answer to that question is in the OP. But in short its better to have less contams due to pH swings. But a jar fully colonized by contams will still work, I have tested that.
-------------------- ¯\_(ツ)_/¯
Edited by Tmethyl (06/13/13 03:14 PM)
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ConcreteWaves



Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 1 year, 2 months
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Re: Quick report on reusing contaminated spawn jars [Re: Tmethyl]
#18414193 - 06/13/13 03:25 PM (10 years, 7 months ago) |
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Quote:
Tmethyl said: The answer to that question is in the OP. But in short its better to have less contams due to pH swings. But a jar fully colonized by contams will still work, I have tested that.
Good shit T
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WanWan
Lurker


Registered: 04/20/17
Posts: 5
Last seen: 10 months, 11 days
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Re: Quick report on reusing contaminated spawn jars [Re: Tmethyl]
#24548204 - 08/12/17 12:52 PM (6 years, 5 months ago) |
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Quote:
Do not attempt to inject water into grain jars before second pressure cooking. All molds will be killed completely with 60mins of 15PSI.
I must question why I cannot inject some more cc of water into the jars to correct for the moisture content lost from the multiple pressure cookings. It should not affect contamination rates as it is being resterilized.
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: Quick report on reusing contaminated spawn jars [Re: WanWan]
#24548252 - 08/12/17 01:17 PM (6 years, 5 months ago) |
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Just because it's not necessary. Plenty of moisture remains even if it doesn't look like it. By adding water you create the risk of the water not soaking back into the grains and puddling in the bottom of your jar, and for no good reason because the jar would have performed fine either way. There is no real upside.
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