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InvisibleHypnotoadCroaked
Retired, but will check MSGs


Registered: 01/05/13
Posts: 1,168
Re: Quick report on reusing contaminated spawn jars [Re: Brain Fart]
    #17955472 - 03/14/13 03:13 PM (10 years, 10 months ago)

Quote:

Brain Fart said:
I'm sorry but no website is cooler then the shroomery. even if people can be butt plugs



Ive already "ignored" one of the usual Parrots.  There are times that I find that repeatedly regurgitating someone else's observations and studies abhorrent.

But I digress.  You are TOTALLY correct Brain Fart.  This is a spectacular site, with great resources and a stellar group of "volunteers"....We are all volunteers here btw.


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OfflineJenooba
Male


Registered: 09/27/12
Posts: 23
Loc: Hilo, Hawaii
Last seen: 7 years, 7 months
Re: Quick report on reusing contaminated spawn jars [Re: HypnotoadCroaked]
    #17955800 - 03/14/13 04:23 PM (10 years, 10 months ago)

Looking great, Tmethyl. I haven't had any issues with contaminants yet, but I'll have to consider this as an option when I do. As has been said, please keep us updated on the results! :thumbup:

You rock, man. :peace:


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Life is all about perspective; don't get too caught up in the one you're experiencing right now.


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Offlinehyphal
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Registered: 02/22/13
Posts: 26
Loc: Planet Earth
Last seen: 10 years, 10 months
Re: Quick report on reusing contaminated spawn jars [Re: HypnotoadCroaked]
    #17955853 - 03/14/13 04:35 PM (10 years, 10 months ago)

Quote:

Whippy said:
Quote:

Brain Fart said:
I'm sorry but no website is cooler then the shroomery. even if people can be butt plugs



Ive already "ignored" one of the usual Parrots.  There are times that I find that repeatedly regurgitating someone else's observations and studies abhorrent.

But I digress.  You are TOTALLY correct Brain Fart.  This is a spectacular site, with great resources and a stellar group of "volunteers"....We are all volunteers here btw.




Agreed, Whippy. We have a saying in horticulture that "plants don't read books or listen to expert horticulturists." I think it apropos here, as well. The myc just wants to run...


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Surfing the Wood Wide Web


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OfflineMushGrooveFarms
Extra-Terrestrial


Registered: 01/01/13
Posts: 100
Last seen: 7 years, 11 months
Re: Quick report on reusing contaminated spawn jars [Re: hyphal]
    #17956204 - 03/14/13 05:42 PM (10 years, 10 months ago)

Funny, I was wondering if this would be successful as I logged on. Perfect timing. Five jars in the PC now. My print was contammed with trich, so I won't be re-innoculating with that. But it seems I will be able to save my grain! Huzzah! Time to get into agar for those dirty spores.


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:cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster::cookiemonster:


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Invisibleshroom-a-zoom
Stranger in a strange land


Registered: 02/26/13
Posts: 414
Loc: Fungiland Flag
Re: Quick report on reusing contaminated spawn jars [Re: MushGrooveFarms]
    #18008198 - 03/25/13 11:51 AM (10 years, 10 months ago)

I just so happen to have 3 jars with some green in them.
I can give this a try if I have the time in the next day or two. If not, they are going outside. I will post either way.
Peace
SAZ


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How it should & shouldn't look - NEW CULTIVATORS GUIDE

:imo:Lets get rid of the SWIM shit, This also goes for my dog, someones pet ferret, and any other way of saying you are not really you.
Its bullshit and we all know it. Man up and admit you are the SWIM.:2cents:


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Invisibleshroom-a-zoom
Stranger in a strange land


Registered: 02/26/13
Posts: 414
Loc: Fungiland Flag
Re: Quick report on reusing contaminated spawn jars [Re: shroom-a-zoom]
    #18045356 - 04/02/13 07:53 AM (10 years, 9 months ago)

Quote:

shroom-a-zoom said:
I just so happen to have 3 jars with some green in them.
I can give this a try if I have the time in the next day or two. If not, they are going outside. I will post either way.
Peace
SAZ





I have not had time since this happened so the jars still sit as is and nasty. I dont think I am going to be trying to reuse these.
:shrug:


--------------------
How it should & shouldn't look - NEW CULTIVATORS GUIDE

:imo:Lets get rid of the SWIM shit, This also goes for my dog, someones pet ferret, and any other way of saying you are not really you.
Its bullshit and we all know it. Man up and admit you are the SWIM.:2cents:


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Offlinemushmybush
Stranger
Registered: 06/11/13
Posts: 45
Last seen: 10 years, 6 months
Re: Quick report on reusing contaminated spawn jars [Re: cherry_darling]
    #18413396 - 06/13/13 12:37 PM (10 years, 7 months ago)

What happens to the bacteria in a PC when the temp gets high enough to a point where weak molecular bonds such as hydrogen bonding and ionic bonds break to form covalent bonds in the wrong places.  This is denaturation in an organism.  Structure and function are highly correlated together so when you break the structure of the bacteria (killing it) it is unable to function (live.)


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 7 days
Trusted Cultivator
Re: Quick report on reusing contaminated spawn jars [Re: mushmybush]
    #18413417 - 06/13/13 12:41 PM (10 years, 7 months ago)

not all bacteria/molds will be killed in a pc, what we call sterile isn't really even close, just gives us a jumpstart on competeing organisms long enough for our mycelium to get a foot hold


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineConcreteWaves
Male


Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 1 year, 2 months
Re: Quick report on reusing contaminated spawn jars [Re: cronicr]
    #18413653 - 06/13/13 01:32 PM (10 years, 7 months ago)

Ok so - >.> I've been wondering this myself Tmethyl everytime i get a contaminated jar. So is this entire method stating...ur PCing the jar WITH the mold inside of it >.> taking it out, and just G2G xfering more culture into it, or knocking it up w/ syringe?

If this is the case....

:wouldyoulookatthat:

where the fuck does the mold go >.> Gas exchange through the polyfil? Shouldnt u change ur polyfil then afterwords....

In my mind....even water evaporates into a gas. A full on mold culture wont just disappear, it goes somewhere >.>


Or are u talking about PCing the moldy jar, taking it out, cleaning it, and having a fresh jar for new grains and culture?


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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Quick report on reusing contaminated spawn jars [Re: ConcreteWaves]
    #18413691 - 06/13/13 01:42 PM (10 years, 7 months ago)

He leaves the moldy grains in the jar and reinoculates the jar after re-sterilizing.

The mold and spores get killed well below 15psi.

The healthy culture just colonizes right over it, it's all dead organic matter to the myc.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineConcreteWaves
Male


Registered: 01/08/13
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Loc: United States
Last seen: 1 year, 2 months
Re: Quick report on reusing contaminated spawn jars [Re: FrankHorrigan]
    #18413727 - 06/13/13 01:50 PM (10 years, 7 months ago)

Quote:

FrankHorrigan said:
He leaves the moldy grains in the jar and reinoculates the jar after re-sterilizing.

The mold and spores get killed well below 15psi.

The healthy culture just colonizes right over it, it's all dead organic matter to the myc.





But any MYC that was in the jar gets destroyed aswell, that's why we add new culture to the jar, correct? At those temps, nothing survives aye?


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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Quick report on reusing contaminated spawn jars [Re: ConcreteWaves]
    #18413731 - 06/13/13 01:51 PM (10 years, 7 months ago)

Yep :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Invisibletbagtag
Boomer Barron

Registered: 01/16/13
Posts: 1,432
Loc: Amsterdam Flag
Re: Quick report on reusing contaminated spawn jars [Re: FrankHorrigan]
    #18413841 - 06/13/13 02:14 PM (10 years, 7 months ago)

My PB thread was based on Tim's suggestion to re-pc. I'm on my 3rd flush of some super potent cubes and my yield was the highest ever. The re-pc doesn't have any adverse effects so far.


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OfflineTmethylM
Smear in the shale
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Registered: 07/16/12
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Re: Quick report on reusing contaminated spawn jars [Re: tbagtag]
    #18413916 - 06/13/13 02:27 PM (10 years, 7 months ago)

Thanks for the help Frank.

Well done tbag, I still haven't experienced any down side to this practice either.
The warnings in the OP are just logical speculation, they likely lose some moisture so they are likely less optimal than the first round.


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¯\_(ツ)_/¯


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OfflineConcreteWaves
Male


Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 1 year, 2 months
Re: Quick report on reusing contaminated spawn jars [Re: FrankHorrigan]
    #18414083 - 06/13/13 03:05 PM (10 years, 7 months ago)

Quote:

Tmethyl said:
Thanks for the help Frank.

Well done tbag, I still haven't experienced any down side to this practice either.
The warnings in the OP are just logical speculation, they likely lose some moisture so they are likely less optimal than the first round.



Quote:

FrankHorrigan said:
Yep :thumbup:





would u 2 suggest only doing this with jars that have observed mold SPOTS coming in,  not a jar that's been almost overrun with it?  I mean, i guess i can answer my ownquestion aye, thats common sense. But you think it would work on a jar that's got alot of colonized mold?


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OfflineTmethylM
Smear in the shale
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Re: Quick report on reusing contaminated spawn jars [Re: ConcreteWaves]
    #18414124 - 06/13/13 03:13 PM (10 years, 7 months ago)

The answer to that question is in the OP.
But in short its better to have less contams due to pH swings.
But a jar fully colonized by contams will still work, I have tested that.


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¯\_(ツ)_/¯


Edited by Tmethyl (06/13/13 03:14 PM)


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OfflineConcreteWaves
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Re: Quick report on reusing contaminated spawn jars [Re: Tmethyl]
    #18414193 - 06/13/13 03:25 PM (10 years, 7 months ago)

Quote:

Tmethyl said:
The answer to that question is in the OP.
But in short its better to have less contams due to pH swings.
But a jar fully colonized by contams will still work, I have tested that.




Good shit T

:hellyeah:


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OfflineWanWan
Lurker
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Registered: 04/20/17
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Re: Quick report on reusing contaminated spawn jars [Re: Tmethyl]
    #24548204 - 08/12/17 12:52 PM (6 years, 5 months ago)

Quote:

Do not attempt to inject water into grain jars before second pressure cooking. All molds will be killed completely with 60mins of 15PSI.




I must question why I cannot inject some more cc of water into the jars to correct for the moisture content lost from the multiple pressure cookings.
It should not affect contamination rates as it is being resterilized.


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OfflineTmethylM
Smear in the shale
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Re: Quick report on reusing contaminated spawn jars [Re: WanWan]
    #24548252 - 08/12/17 01:17 PM (6 years, 5 months ago)

Just because it's not necessary. Plenty of moisture remains even if it doesn't look like it. By adding water you create the risk of the water not soaking back into the grains and puddling in the bottom of your jar, and for no good reason because the jar would have performed fine either way. There is no real upside.


--------------------
¯\_(ツ)_/¯


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