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OfflineTmethylM
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Registered: 07/16/12
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Quick report on reusing contaminated spawn jars * 3
    #17951157 - 03/13/13 08:36 PM (10 years, 10 months ago)

Wanted to make a quick report on this while I have it in my mind. This is not a tek.
I've seen it debated, and I've seen experienced opinions shared on the subject, but what I had never seen was any actual experimentation.
It has been argued as a lost cause, and to toss it. The idea of pressure cooking the contams and inoculating the jars again was also used multiple times.
Here is some actual experimentation.


This is a pic of 7 heavily contaminated spawn jars, they were contaminated with everything from bacteria, to Dactylium, to multiple Aspergillus sp.
They were the result of using wild-found spores from multiple species, and some contaminated P.cubensis.


Pressure cooked them for 60 minutes @ 15 PSI, here is a pic of the same jars(and one extra), 8 days after clean MS inoculation:


Some minor details, I did this with 11 jars total over the course of 3 weeks, and all of the jars that were PC'ed and re-inoculated have a 100% success rate thus far. I did shake the moldy jars like crazy before PC'ing them. Keep in mind you want to PC the contams at first sight instead of allowing them to get out of hand, because they can cause dramatic changes in the pH, thus causing issues with the second inoculation/colonization. The jars which would have been thrown away are performing with no problems, although they would have likely performed better before the second PC'ing, it's hard to complain seeing the results in person.


A quick rundown of the entire process:


Prepared spawn jars.
Pressure cooked spawn jars for 100mins @ 15psi.
Inoculated spawn jars.
7 jars contaminated days later.
Pressure cooked contaminated spawn jars for 60mins @ 15PSI.
Inoculated previously-contaminated-double-pressure-cooked spawn jars.
Healthy mycelium, no contamination, grains not too dry, good colonization speeds maintained.

Closing statement; do not open contaminated jars before pressure cooking. The double PC'ed jars will likely not perform as well as the single PC'ed uncontaminated jars. Do not attempt to inject water into grain jars before second pressure cooking. All molds will be killed completely with 60mins of 15PSI.


--------------------
¯\_(ツ)_/¯


Edited by Tmethyl (03/13/13 10:09 PM)


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