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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: Isolates Vs. Clones [Re: mel0n420]
    #17947820 - 03/13/13 07:08 AM (10 years, 11 months ago)

Quote:

mel0n420 said:
Well there is actually a lot of evidence around that shows some clones can outperform isolates.





That's just stating the obvious, although you should have said some clones can outperform some isolates.

A clone from a crappy flush will produce crappy offspring, but they're still going to be better than a non-fruiting isolate.

Not every isolate will fruit or fruit well.  However, the stellar isolates will far outperform anything else.  The idea is to isolate strains on agar an fruit them all in a controlled strain test.  Some won't produce anything.  Some will produce a little and some will blow your mind with wall to wall flushes.  You save these isolates on master culture slants and grow them the rest of your lives.  Some of my very best shiitake strains are over 30 years old.

Quote:

cronicr said:
isolate from a clone and theres not much hassel:shrug:




Nor will there likely to be any more mushrooms.  If a fruit has multiple strains active, removing one might just ruin the whole thing since they're working in concert.
RR


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OfflineRogerRabbitM
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Re: Isolates Vs. Clones [Re: San]
    #17947826 - 03/13/13 07:11 AM (10 years, 11 months ago)

Quote:

San said:
If you're picking the actual fruiting body and cloning it from an internal tissue sample then it's probably isolated. Rather unlikely that you would get multiple growths from that, but looking at it on agar is the best way to tell.




Bad info.  It's extremely common for more than one strain to be active in a fruitbody.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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InvisibleBrain Fart
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Registered: 12/19/07
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Re: Isolates Vs. Clones [Re: RogerRabbit]
    #17948035 - 03/13/13 08:54 AM (10 years, 11 months ago)

Quote:

RogerRabbit said:
Quote:

San said:
If you're picking the actual fruiting body and cloning it from an internal tissue sample then it's probably isolated. Rather unlikely that you would get multiple growths from that, but looking at it on agar is the best way to tell.




Bad info.  It's extremely common for more than one strain to be active in a fruitbody.
RR





Ya I read that and was like ehhhhhhhhhh I don't think so...thanks for clarifying RR.


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