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Amazon Shop: ½ Pint Jars, Paul Stamets

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Offlinestoner2002
Stoner

Registered: 11/15/02
Posts: 183
Last seen: 14 years, 3 months
Is there a tek or a good thread on this?
    #1794162 - 08/09/03 12:58 AM (14 years, 8 months ago)

I want to start by innoculate some pint jars using the popcorn grain spawn tek.  I want to end up with the straw log tek.  Where I'm confused is what I should do in between those two teks to get a bunch of quart jars colonized. 

I was thinking of doing the popcorn spawn tek again with the bigger jars but I dont know if I can do a grain to grain transfer?  I mean once the quart jars are done in the PC and ready to be inoculated, can I simply take the lid off the jars and mix in some of the colonized substrate with my hands?  Does it have to be nice and evenly mixed? I'd wear gloves ofcourse. 

I'm thinking of building a little teepee sort of thing out of bed sheets to minimize air flow so I can try something like this.  I'd spray a ton of that lysol for air born bacteria in the bed sheet "room" and the actual room, spray everthing down with lysol, shut off the AC, not breath, generally try and be as obsessive about being steril as possible. :smile:

Also, I dont want to start with inoculating quart jars with syringes as that would take more syringes than Id be willing to do.

Throw any suggestions or comments my way, I'd really appreciate it.

Peace


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: Is there a tek or a good thread on this? [Re: stoner2002]
    #1794240 - 08/09/03 01:35 AM (14 years, 8 months ago)

read everything

i am not kidding you, the answers to all of your questions are right there .


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OfflinePsilocybin_monkey
Shroomer

Registered: 06/19/03
Posts: 1,340
Loc: Dragon's Den
Last seen: 6 years, 6 months
Re: Is there a tek or a good thread on this? [Re: deanofmean]
    #1794245 - 08/09/03 01:36 AM (14 years, 8 months ago)

^^^ yeah read it all ^^^


--------------------
Welcome to my world!



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OfflineDemiurge
creator ofworlds

Registered: 04/11/03
Posts: 693
Loc: Florida
Last seen: 7 years, 1 month
Re: Is there a tek or a good thread on this? [Re: Psilocybin_monkey]
    #1794274 - 08/09/03 01:49 AM (14 years, 8 months ago)

The tee pee you are talking about is called a "glove box." Lots of people use them for grain to grain transfers. Except I wouldn't make one out of bedsheats. Look in the faq or do a search to learn how to build one properly.

What's wrong with doing quart jars with syringes? ONE syringe will inoculate about TEN quart jars, which is plenty of spawn for a straw log.


--------------------
Just another animal embeded in a technological coral reef extruded psychic objects...


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Offlinestoner2002
Stoner

Registered: 11/15/02
Posts: 183
Last seen: 14 years, 3 months
Re: Is there a tek or a good thread on this? [Re: Demiurge]
    #1794395 - 08/09/03 02:57 AM (14 years, 8 months ago)

"once the quart jars are done in the PC and ready to be inoculated, can I simply take the lid off the jars and mix in some of the colonized substrate with my hands? Does it have to be nice and evenly mixed?"

So I can do this?

I'm really tired but ill read all the sterilization techniques tommorow.


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OfflineSev
Astropath
Registered: 06/06/03
Posts: 1,426
Loc: NY
Last seen: 3 years, 6 months
Re: Is there a tek or a good thread on this? [Re: stoner2002]
    #1794435 - 08/09/03 03:20 AM (14 years, 8 months ago)

I'd advise the use of plastic gloves and some alcohol to sanitize them before you do it, but sure.


--------------------
"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

All of my posts are full of fiction and blatant lies.


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: Is there a tek or a good thread on this? [Re: stoner2002]
    #1794508 - 08/09/03 04:02 AM (14 years, 8 months ago)

if your'e thinking of doing a project such as this, do yourself a favor, and buy the TMC .
The Shroomery Book Store 
answers to all your questions . :smile:


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OfflineDemiurge
creator ofworlds

Registered: 04/11/03
Posts: 693
Loc: Florida
Last seen: 7 years, 1 month
Re: Is there a tek or a good thread on this? [Re: deanofmean]
    #1795316 - 08/09/03 02:59 PM (14 years, 8 months ago)

Yes, you can do this. It is called a grain to grain transfer. Read about it in the faqs and teks. There is lots of information already available. Be fast, and worry about getting it evenly mixed after you re-seal the jars. Just give them a good shake and the grain should break up fine. It would be advisable to do it inside a "glove box" though. There is lots of information available on building one. You just have to look around.


--------------------
Just another animal embeded in a technological coral reef extruded psychic objects...


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Offlinestoner2002
Stoner

Registered: 11/15/02
Posts: 183
Last seen: 14 years, 3 months
Re: Is there a tek or a good thread on this? [Re: Demiurge]
    #1795749 - 08/09/03 05:33 PM (14 years, 8 months ago)

I love you guys :smile:


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Invisiblegeorge castanzaM
Lord Of The Idiots!
I'm a teapot User Gallery Arcade Champion: BurgerTime, Space Invaders
Registered: 10/21/02
Posts: 7,542
Trusted Cultivator
Re: Is there a tek or a good thread on this? [Re: stoner2002]
    #1795804 - 08/09/03 05:51 PM (14 years, 8 months ago)

good luck b/c your going to need it...


--------------------
Kramer Cakes


[url=[url=

   

:pm:


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Offlinestoner2002
Stoner

Registered: 11/15/02
Posts: 183
Last seen: 14 years, 3 months
Re: Is there a tek or a good thread on this? [Re: george castanza]
    #1796251 - 08/09/03 08:36 PM (14 years, 8 months ago)

I dont know man. Im good at being anal retentive and following directions verbatim. Those contams can kiss my ass.


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Invisiblegeorge castanzaM
Lord Of The Idiots!
I'm a teapot User Gallery Arcade Champion: BurgerTime, Space Invaders
Registered: 10/21/02
Posts: 7,542
Trusted Cultivator
Re: Is there a tek or a good thread on this? [Re: stoner2002]
    #1796276 - 08/09/03 08:45 PM (14 years, 8 months ago)

all kramer wants you to know that if your doing a grain to grain tranfer you had better be sterile and he is talking hospital


--------------------
Kramer Cakes


[url=[url=

   

:pm:


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Amazon Shop: ½ Pint Jars, Paul Stamets

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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