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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: Machiavelliavore]
#22446362 - 10/28/15 07:50 PM (9 years, 2 months ago) |
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Spaghetti sauce jars work if you can get those.
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AMU Q&A
If you don't have a plan of your own, you'll become a part of somebody else's.
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CorteX_


Registered: 07/26/05
Posts: 147
Loc: The Hague, The Netherlands
Last seen: 26 days, 22 hours
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: Machiavelliavore]
#22446626 - 10/28/15 09:03 PM (9 years, 2 months ago) |
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I was thinking about using these Nutella jars: http://images3.sanalmarket.com.tr/images/1000/urun//large/07155709_large.jpg I have a couple of these sitting here already, the lids don't seem to have a recycling code on them but they are from a pretty hard plastic and seem very sturdy. I hope they'll survive my PC.
Grainwater agar looks cool. How long does it take from a spore solution on agar to be transferable to another medium? Because when I'm preparing my grain water I will have to use the rye I prepared asap to avoid contamination. I'm not sure if waiting a couple days or a week before being able to inoculate the jars containing the rye is a good idea.
Actually, I found 2 Asian supermarkets close by that both carry agar and in all kinds of colors too!
Thanks for all the answers, really appreciate it
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: CorteX_]
#22446801 - 10/28/15 09:36 PM (9 years, 2 months ago) |
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From what info I could find, those containers are made of Polyethylene Terephthalate, melting point 260C, so you should be good. Dunno if the lids are the same. I guess you'll find out 
If you can find it, the little packets of agar powder are way easier to deal with than the bars or pasta looking versions. It distributes much better. Telephone brand is one.
You'll be fine leaving rye jars for a week. I've had perfectly fine results from grains left over a month, as long as the filter isn't permiable enough to dry them out.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent.
Triggered yet?
Only a square would say "a cube is a cube."
No, this does not look right...
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mazeze
Stranger
Registered: 10/31/15
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: Machiavelliavore]
#22459731 - 10/31/15 10:40 PM (9 years, 2 months ago) |
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first of all thanks for your wonderful tek!! A few questions if I may:
1. here are 2 photos of the same jar (different sides) of mexicana, 4 months after inoculation with spores. Does it look ready? One side looks full of sclerotia but the other is mostly white. Should I wait more?


2. If each jar yields upwards of 100g fresh as you say, I won't be able to use it all at once. Storing in the fridge is good for a week or two, but what if I can't consume it even in that timeframe? Should I dry them (I have experience doing that with mushroom (fruit) but is it OK with slecrotia as well? I don't see much about drying truffles on the forums so I am interested in your opinion), or ...
3. Can I remove only a few truffles from the top of the jar (as opposed to using a bike tire to break it and remove everything) and let the rest continue to live in the jar? Or will that injure the mycelium and risk the survival of the rest?
thanks a million!!!
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StickyIcky Fingers
Free DOOM!! Available Here

Registered: 12/03/13
Posts: 178
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: mazeze]
#22461072 - 11/01/15 08:56 AM (9 years, 2 months ago) |
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Either would be fine I like to let mine go at least 8-10 months but in the past I have harvested after several months (in a SAB with gloves on) and put the grains back in the jar and around 10cc of water and had them bounce back and produce more stones. If you try this watch closely for contamination the first couple weeks after. But the combined amount from the two harvests was not larger than harvesting a jar once after 8-12 months.
Drying is one option I think people use dehydrators for this and it would probably be faster if you sliced up the larger stones. I have read reports of stones losing potency when stored dry and they are also very hard ..... like a stone once dry. At least one person on here makes chocolates with ground up dry stones for storage. To store mine I have made a batch of mead with some and after the last harvest I just chopped em up and filled a quart jar to the brim with fresh stones and honey.
Yes you could probably manage that if done in a SAB and watched carefully for contamination afterwords. Sometimes all the stones will be in the bottom half of the jar especially if the jar is a bit on the dry side (like if I use a jar lid with multiple small holes and a whole SFD instead of a jar lid with a single hole with an SFD patch siliconed on) IME.
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LuzaW
Um, yeah


Registered: 11/10/15
Posts: 610
Loc: Southeast USA
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: StickyIcky Fingers]
#22568890 - 11/24/15 03:51 PM (9 years, 1 month ago) |
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I am still unclear about how to use the tire referenced a couple of times in this tek. What exactly am I to do with it? I am stumped.
As a side note, your local Goodwill store might be a good source for jars, plastic bottles, tubs, lamp fixtures, etc. that you may need for a grow project.
Violet, thank you for the write up. I inoculated 7 qts with ATL#7 today following it.
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blackout


Registered: 07/16/00
Posts: 5,266
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: LuzaW]
#22569503 - 11/24/15 05:50 PM (9 years, 1 month ago) |
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Quote:
LuzaW said: I am still unclear about how to use the tire referenced a couple of times in this tek. What exactly am I to do with it? I am stumped.
Smack the jar against it. It gives the grains a good jolt but should not crack the jar.
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LuzaW
Um, yeah


Registered: 11/10/15
Posts: 610
Loc: Southeast USA
Last seen: 1 year, 8 months
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: blackout]
#22570060 - 11/24/15 07:34 PM (9 years, 1 month ago) |
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Thanks blackout - mystery solved!
I appreciate that you typed that real slow so I could keep up...
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: LuzaW]
#22570821 - 11/24/15 10:36 PM (9 years, 1 month ago) |
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I have to hold it by the side of the jar. I try to hold it by the side of the jar and brace it with the hand I'm hitting it with.
... It kindof sucks.
I'm not sure if I have mentioned it in this thread, but personally I no longer use glass jars as a normal, in favor of the Ziploc twist-top containers. A jar has broken in my hands on no fewer than 3 occasions and after 2 years of glass's general hassle I realized that I hate it for frequent use. Now another 2 years later using plastic and I'm still 100% on that. I never much liked siliconed lids either, and I would love to leave SFDs out of the equation. Instead, it COULD be done with nothing but Ziploc containers from a store and local rye, wheat, popcorn, milo, or if necessary birdseed, along with the the pressure cooker, basic still-air tote and source of culture.
But in the end, the tek has been purposefully left the way it is because of the ease of a person who wants to fallow instructions for basic methods and minimal sterile workspace stuff. A batch of jars for sealed and filtered use with SFDs and silicone make for a reliable no-trick setup.
The change with Ziplocs is leaning to use the cracked loose lid lightly unscrewed to sterilize un-sealed, and if necessary *as is the case with growing for sclerotia* allowing just enough exchanged air around the threads and seal for the mycelium to breathe enough to have lots of truffle growth, by to mention alkaloid production and storage, but not enough to have any significant or detrimental moisture loss in the container. According to my preference, they can be filled to around 800mL and still be easy. This means the surface of the grains is just about 1/5 from the top of the jar.
Breaking up a super consolidated cake in one of these is not difficult like in a jar since it is not rigid to a fault with a sharp and volatile breaking point of stress. They can be broken on the side if needlessly abused but you won't hurt your hand in them, and knocking them by the curved edge of the bottom gives the content a gold jolt without stressing the sides of the containers so that they don't break at all.
But best of all, they don't have to be broken up for harvest. The widest point of the container is the opening at the lid, so the cake just slides out with no problem. It's awesome. There are many other advantages to the container. The "trick" with using them, once understood in the very basic and simple way, quickly becomes an advantage in allowing the containers to be used in a wide variety of great ways!
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!
PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
• Violet's Teks and Posts •
Edited by Violet (11/25/15 07:36 AM)
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psylo-not
Stranger


Registered: 04/27/11
Posts: 306
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: Violet]
#22579906 - 11/26/15 08:41 PM (9 years, 1 month ago) |
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When isolating, would you clone from the fruit or the stones? Or does it depend on what you're looking to do with it? I have an isolate from my first fruit to form (also clustered) and plan to clone the largest stone from my first jars to compare but wondering if anyone has done this yet?
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: psylo-not]
#22583233 - 11/27/15 05:05 PM (9 years, 1 month ago) |
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I always clone the stones. Fruits can be severely difficult to clone, because they have such small stems. And as they're almost never grown in clean conditions, this makes getting a clean biopsy or tissue sample near impossible at least for beginners. Not to say it can't be done... Apparently You managed, which is cool. But regardless, cloning the stones is EASY! With a scalpel, 16ga needle tip, or even sterilized tweezers or flathead screwdriver, you can break a nicely sized stone open and scrape out a bit of the inside to drop on agar. Good truffle strains can often be good fruiters too. Test stone clones taken from jars with different multi-spore sources to find the better stone formers, and then if you like try them out to find the good fruiters!
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!
PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
• Violet's Teks and Posts •
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 1 year, 2 months
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: Violet]
#22583302 - 11/27/15 05:22 PM (9 years, 1 month ago) |
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You can easily clone sclerotia, I have done it in open air before. As its more solid you can pass a needle through it and take out a "core" like coring an apple. If you have some water in your syringe it will easily push the tissue back out. Some have cored cubensis too.
You can wash the outside with peroxide or just cut 2 sides off and quickly take a core.
you can see the thin core suspended on the right here, after being swirled up.

This was from commercial sclerotia.

Aero had great success cloning commercial sclerotia. I did not have as good luck. For those who can source it the commercial ones are usually cheaper than buying spores, and they have hopefully isolated a good strain.
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psylo-not
Stranger


Registered: 04/27/11
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: blackout]
#22585459 - 11/28/15 09:16 AM (9 years, 1 month ago) |
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Thank you for your replies, good advice on both!
I tried to get the inside of the stem for the clone, but as you said bit too small, so I took a bit of the entire stem from just underneath the cap as I thought it might shield the contams slightly. 1st plate had 1 tiny spot of bacteria, but second and third came up clean.
When you say different MS source, do you mean vendor, or simply different injection rather than a master G2G? I currently have 3 jars from MS, different injections but all from same syringe. I'm guessing those 3 would be genetically different from each other as they had different spores combine, correct?
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: psylo-not]
#22585968 - 11/28/15 11:33 AM (9 years, 1 month ago) |
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Yeah, they will be genetically different from the spore syringe. It could also be done by taking different sectors or sections from a multispore agar plate.
Starting from a different spore print can also be helpful sometimes if you're having real problems getting something prolific.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent.
Triggered yet?
Only a square would say "a cube is a cube."
No, this does not look right...
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: Machiavelliavore]
#22586080 - 11/28/15 12:04 PM (9 years, 1 month ago) |
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Right. I could have elaborated more, but I used the phrase from different multi spore sources because doing grain-to-grain transfers from one jar tends to spread the dominant and fast-colonizing mycelium the most - taking clones from two jars that had the same master jar is as likely to result in the same cultures as not. Doing those transfers and cloning instead of cloning from the original multi-spore jar is preferable because it does have that effect of breaking the faster strains away from the pack. I have more often than not I have gotten stone formation faster from g2g-inoculated jars than the sets of original multi-spore jars. It helps give the better strains an opportunity to spread so you're much more likely to catch them in a clone. A sort of easy selection to test better cultures.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!
PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
• Violet's Teks and Posts •
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psylo-not
Stranger


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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: Violet]
#22591012 - 11/29/15 03:23 PM (9 years, 1 month ago) |
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Great! Makes total sense, this helped a bunch!
I got my scalpel ready to go for this guy!
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dlynn
Stranger

Registered: 10/05/15
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: psylo-not]
#22597153 - 11/30/15 11:33 PM (9 years, 1 month ago) |
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I can't wait to use this tek. Good job!
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BasilBush
Reality Bender



Registered: 11/13/13
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: dlynn]
#22602816 - 12/02/15 04:02 AM (9 years, 1 month ago) |
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Got 3 truffles kits on the way to me should be here today at some point once there done I'm defo gonna core the best looking stones and give this tek a go. Anyone have an idea of what sort of mushrooms if any would come from Pandora Truffles. Great easy to read tek much respect to violet and blackout to for some good tips
-------------------- I HUNT MAGIC MUSHROOMS AND MAKE MIND BLOWING TEA,
I BUY ROOT BARK OFF THE INTERNET AND EXTRACT DMT,
SMOKE MY FRIENDS CHANGA IT TASTES BETTER FOR FREE
TRIPPING IS WONDERFUL IT HELPS ME FIND PEACE,
SO WILL YOU COME ALONG TO MY NEXT EUPHORIC FEAST.
I LOVE MUSHROOMS THEY MUSH UP THE ROOM THERE AINT MUCH ROOM IN HERE
SPECIAL GREEN TEA MADE FROM A CACTUS TREE SETS YOUR MIND AND SPIRIT FREE
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LuzaW
Um, yeah


Registered: 11/10/15
Posts: 610
Loc: Southeast USA
Last seen: 1 year, 8 months
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: BasilBush]
#22623261 - 12/06/15 08:09 PM (9 years, 1 month ago) |
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I started my jars Nov 24, all 7 are working but a little slow. Ambient temps are just at 70 F, maybe a little below.
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: TRUFFLE TEK - Complete guide to psychedelic sclerotia and their mushrooms [Re: LuzaW]
#22710570 - 12/28/15 08:00 PM (9 years, 21 days ago) |
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Bump!
Gave this old guy a few much-needed little updates!
For one, I had referred to "glovebox" instead of "still air box" throughout the inoculation directions
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!
PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
• Violet's Teks and Posts •
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