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mycofever
Part-Time



Registered: 10/13/12
Posts: 605
Loc: USA
Last seen: 5 years, 1 month
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mycelium transfers and aging?
#17893040 - 03/02/13 04:26 PM (11 years, 2 months ago) |
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Does the strain that you are working with get weaker after each transfer that you make. For instance you have a strain that colonized on a petri-dish. You then transfer it onto a slant. Then once a year you transfer it to a new slant.If you decide to grow from that strain you would transfer from the slant to a petri-dish then to grain. Through all of these transfers to keep a strain for long term does it get weaker. Or does the strain only become weaker through each time that it has been fruited and cloned. Is there some literature that will give me a greater understanding of this . I read this from fp and it has me wanting to get a grip on this.This is a quote from fp.
"The first time a wild species is tissue cultured, the age is denoted as P-O. Thereafter each successive growth over the petri dish's surface is described in increments of P-l, etc. This library tries to maintain strains closest to their wild origins or closest to their peak fruiting potentials". Also I read in one of RR's posts that he is still using strains from the 90's How would that strain still be strong that would make it like a P-15 or P-20 as fp labels them. Can someone please enlighten me. Thanks...
-------------------- Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
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Re: mycelium transfers and aging? [Re: mycofever]
#17894356 - 03/02/13 08:48 PM (11 years, 2 months ago) |
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The number of cell divisions determines to a large extent the amount of senescence. Chronological time also plays a role. Nobody allows mycelium to completely crawl over the surface of a petri dish, or at least they shouldn't.
You don't grow to fruiting and then clone. Once you have a culture, you simply go back to those young cell lines for the next crop. If you're careful, senescence will never set in. Some of my very best commercial strains have been my possession over 30 years. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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mycofever
Part-Time



Registered: 10/13/12
Posts: 605
Loc: USA
Last seen: 5 years, 1 month
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Re: mycelium transfers and aging? [Re: RogerRabbit]
#17895964 - 03/03/13 06:36 AM (11 years, 2 months ago) |
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Thanks for that RR. This has been helpful. I have shiitake 75, shiitake ts-1, A wild oyster strain,King oyster and Ganoderma tsugae-AM1. I have all of these in their youngest stages.I want to keep them that way.I have them in slants and some on petri-dishes waiting to be moved to slants.
-------------------- Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.
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Terry M
Stranger in a Strange Land



Registered: 06/18/10
Posts: 1,502
Loc: Rhode Island
Last seen: 9 years, 3 months
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Re: mycelium transfers and aging? [Re: mycofever]
#17901815 - 03/04/13 09:34 AM (11 years, 2 months ago) |
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The trick I use to minimize age when transferring from an agar plate to a slant, for example, is to cut a colonized agar square when the growth circle is still relatively small and young. Then, I seal the source plate back up with parafilm, and let it continue to grow. If any contamination develops on this plate, my transferred growing slant is then junked, for it may be contaminated. If it isn't contaminated, I have get a nice fully grown plate (minus a little cutout) to store or transfer to grain.
-------------------- Liberté, égalité, humidité.
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