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Offlinetheonlysun81
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mushroom research
    #17885359 - 03/01/13 09:42 AM (11 years, 2 months ago)

I am currently an environmental resources management major and am interested in doing some kind of research on mushrooms in either a bioremediation process or as a candidate for use in biofuels as I read somewhere that mushroom breakdown more complex organic compounds and integrate 50% more of these compounds into their structure than bacteria and other plants. I was wondering where I could get started on research for the actual proposal? I know very little about biofuels in general, but am pretty well versed on the bioremediation process. But as far as muchrooms, all I really know is that they can make you trip. If any mycologists know of any specific strains that may have some potential in these areas I feel like that might be a good starting point.


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Offlineomegafaust
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Re: mushroom research [Re: theonlysun81]
    #17885369 - 03/01/13 09:45 AM (11 years, 2 months ago)

There is a mushroom that produces diesel, and i believe oyster mushrooms are used to filter oil spills and the like, and there is a fungus they are using for portable toilets that decomposes human waste


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Offlinetheonlysun81
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Re: mushroom research [Re: omegafaust]
    #17885396 - 03/01/13 09:52 AM (11 years, 2 months ago)

are there specific strains that you know of?


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Offlinetheonlysun81
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Re: mushroom research [Re: theonlysun81]
    #17885408 - 03/01/13 09:55 AM (11 years, 2 months ago)



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Offlineomegafaust
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Re: mushroom research [Re: theonlysun81]
    #17885412 - 03/01/13 09:57 AM (11 years, 2 months ago)

:smile:


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Offlinetheonlysun81
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Re: mushroom research [Re: omegafaust]
    #17885421 - 03/01/13 10:02 AM (11 years, 2 months ago)

Well for research what would I do? Like we know it makes diesel, what would be the hypothesis? Maximizing yields?


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Re: mushroom research [Re: theonlysun81]
    #17885450 - 03/01/13 10:13 AM (11 years, 2 months ago)

You could hypothesize on the commercial ability, or whether fungi played a major role in producing fossil fuels, or on maximizing yeilds.


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Re: mushroom research [Re: omegafaust]
    #17885459 - 03/01/13 10:16 AM (11 years, 2 months ago)

Hmmmmmmm.... I like the commercial ability. It seems like the most applicable/less complicated. So say the I get a grant for $3000 for it. It would be commercially viable if I made more than $3000 worth of diesel right?


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Offlineomegafaust
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Re: mushroom research [Re: theonlysun81]
    #17885469 - 03/01/13 10:19 AM (11 years, 2 months ago)

Absolutely.  However, it requires a lab, and a specific tree I believe.  I'm not positive on the growth parameters, but if it can break down cellulose like they claim, there could be a wide margin of profit, if it were able to be mass produced.  If tou can, try and find a cellulose to diesel ration for the fungus.  Then you can work out the math and see if there is profit.


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Offlinetheonlysun81
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Re: mushroom research [Re: omegafaust]
    #17885472 - 03/01/13 10:20 AM (11 years, 2 months ago)

If it requires a certain tree to grow on than wouldn't it be a better research project, as far as for biofuels go, grow different strains of mushroom and determine the level of triglicerides in them. Or the rate in which they absorb cellulose?


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Offlineomegafaust
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Re: mushroom research [Re: theonlysun81]
    #17885483 - 03/01/13 10:24 AM (11 years, 2 months ago)

Very good idea, also take into account the hydrocarbon levels of different strains.  Although in that article they mention it consuming cellulose and lignen, so it may just originate from that tree, and not require it.


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Re: mushroom research [Re: omegafaust]
    #17885596 - 03/01/13 10:54 AM (11 years, 2 months ago)

And as far as commercial viability, it is a whole hell of a lot more viable to only need to grow mushrooms and not have mushrooms that need a growing medium that is native to Patagonia am I right?

But what I would want to do is have a common control mushroom that is well known and put it against say 3 or 4 different strains. Maybe a novel one, like this one, a couple local ones or others, like oysters, that are already being used. And I want to see how well they uptake and integrate cellulose into their structures. So basically just grow them, maybe have a couple on a grow bed contaminated with hydrocarbons and than do spectroanalysis after they're fully grown to see how they faired.


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Re: mushroom research [Re: theonlysun81]
    #17885620 - 03/01/13 11:02 AM (11 years, 2 months ago)

That sounds like a novel idea.  However, i feel the hypothesis has changed.  Are we hypothesizing on the bio diesel, or simply cellulose uptake?  if you're leaning toward cellulose uptake, G Roseum may not be needed, whereas more common mushrooms may give you better results.  Now if you are comparing hydrocarbon emmissions, then Roseum is still viable.  maybe I just don't get what you're aiming for.


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Re: mushroom research [Re: omegafaust]
    #17885708 - 03/01/13 11:19 AM (11 years, 2 months ago)

Would an increase in cellulose uptake or hydrocarbon emmission make it a better candidate for a biofuel?


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Edited by theonlysun81 (03/01/13 11:19 AM)

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Offlinetheonlysun81
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Re: mushroom research [Re: theonlysun81]
    #17885719 - 03/01/13 11:21 AM (11 years, 2 months ago)

that is a question not a hypothesis


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Offlineomegafaust
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Re: mushroom research [Re: theonlysun81]
    #17885734 - 03/01/13 11:24 AM (11 years, 2 months ago)

Does cellulose uptake effect hydrocarbon emissions?  if so would it be a viable source of biofuel?  then, which mushrooms play the greatest role in this conversion, and what is the best way to commercialize the product?  i feel an excellent paper in the works.


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Re: mushroom research [Re: omegafaust]
    #17885836 - 03/01/13 11:48 AM (11 years, 2 months ago)

yesss I like it. It starts general and gets specific.


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Re: mushroom research [Re: theonlysun81]
    #17885850 - 03/01/13 11:50 AM (11 years, 2 months ago)

But how would I determine if cellulose affects hydrocarbon emmission? would I simply add cellulose to soil and  grow like corn and see if it yields more hydrocarbon?


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Offlineomegafaust
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Re: mushroom research [Re: theonlysun81]
    #17886268 - 03/01/13 01:12 PM (11 years, 2 months ago)

Perphaps use substrates with different cellulose and lignen contents, and measure and compare hydrocarbon emissions?


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OfflineRogerRabbitM
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Re: mushroom research [Re: theonlysun81]
    #17889562 - 03/01/13 10:29 PM (11 years, 2 months ago)

Quote:

theonlysun81 said:
Hmmmmmmm.... I like the commercial ability. It seems like the most applicable/less complicated. So say the I get a grant for $3000 for it. It would be commercially viable if I made more than $3000 worth of diesel right?




I get involved in writing grants such as this through the local conservation district.  You won't get a grant for an idea.  You would first want to produce the fuel through your process to show the grant writers a small-scale model in actual operation.  You could then seek funding to expand to commercial scale production.
RR


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Re: mushroom research [Re: RogerRabbit]
    #17891170 - 03/02/13 08:55 AM (11 years, 2 months ago)

Do you think his ideas are worthy roger?


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OfflineRogerRabbitM
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Re: mushroom research [Re: omegafaust]
    #17891245 - 03/02/13 09:23 AM (11 years, 2 months ago)

Ideas are like something else we all have.  One must act on those ideas to make them work or not work on a small scale.  At that time you can look for funding to take it larger if initial results show promise.

I'm not aware of any commercially viable ways to create fuel with fungi.  I think a more promising area of research would be to harvest metabolites for their plant hormones and fertilizer potential.
RR


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Re: mushroom research [Re: RogerRabbit]
    #17891258 - 03/02/13 09:28 AM (11 years, 2 months ago)

I myself am a worm farmer, and I am currently incorporating mycelium from a very broad range of fungi into my worm beds.  Adding active mycelium to my vermicompost.  A perfect soil ammendment or additive.


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InvisibleAmphibolos
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Re: mushroom research [Re: omegafaust]
    #17892775 - 03/02/13 03:38 PM (11 years, 2 months ago)

You wont beat the refineries that are currently implemented inside Saccharomyces cerevisiae by mere strain selection...


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Edited by Amphibolos (03/02/13 03:38 PM)

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Offlineomegafaust
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Re: mushroom research [Re: Amphibolos]
    #17892789 - 03/02/13 03:40 PM (11 years, 2 months ago)

Who won't


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Re: mushroom research [Re: RogerRabbit]
    #17894469 - 03/02/13 09:13 PM (11 years, 2 months ago)

Quote:

RogerRabbit said:
Ideas are like something else we all have.  One must act on those ideas to make them work or not work on a small scale.  At that time you can look for funding to take it larger if initial results show promise.

I'm not aware of any commercially viable ways to create fuel with fungi.  I think a more promising area of research would be to harvest metabolites for their plant hormones and fertilizer potential.
RR



Which metabolites would this be?


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Offlinetheonlysun81
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Re: mushroom research [Re: RogerRabbit]
    #17894508 - 03/02/13 09:21 PM (11 years, 2 months ago)

Quote:

RogerRabbit said:
Ideas are like something else we all have.  One must act on those ideas to make them work or not work on a small scale.  At that time you can look for funding to take it larger if initial results show promise.

I'm not aware of any commercially viable ways to create fuel with fungi.  I think a more promising area of research would be to harvest metabolites for their plant hormones and fertilizer potential.
RR



And when you say act on those ideas wouldn't that be more for a new process or something of the sort? Like I would need the money to gather the materials to do something that people already know how to do: grow mushrooms and analyze the mushrooms for cellulose content. I'm not trying to see if I can commerically grow these novel mushrooms or try an experimental method to grow mushrooms. I would be building on other people's research.


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Re: mushroom research [Re: Amphibolos]
    #17894553 - 03/02/13 09:30 PM (11 years, 2 months ago)

Quote:

Amphibolos said:
You wont beat the refineries that are currently implemented inside Saccharomyces cerevisiae by mere strain selection...



and I was under the impression that microbes only integrated 20% of consumed organic molecules into their structure and fungi were closer to 50%


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Re: mushroom research [Re: Amphibolos]
    #17895897 - 03/03/13 05:49 AM (11 years, 2 months ago)

Saccharomyces cerevisiae is a yeast, and its metabolical pathways are closer to fungi. Also, it is better known and easy to manipulate. They are also already used to produce alcohols useful as an energy source (biofuels). There is a nice article of the journal nature about this. I can link it to you if you are interested.

Imho, a good fungus to use would be Phanerochaete chrysosporium  since his genome is already sequenced. You would have to overexpress the desired traits for bioremediation. However, i'm sure such strains already exists in the databases, you just have to search for them.

If not, Pleurotus ostreatus is also a good choice. His genome have been sequenced aswell.


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Offlineodbsmydog
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Re: mushroom research [Re: theonlysun81]
    #17913827 - 03/06/13 02:58 PM (11 years, 2 months ago)

Quote:

theonlysun81 said:
nevermind found it
http://phys.org/news144958975.html




whoa!


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Re: mushroom research [Re: Amphibolos]
    #17914809 - 03/06/13 06:07 PM (11 years, 2 months ago)

Quote:

Amphibolos said:
Saccharomyces cerevisiae is a yeast, and its metabolical pathways are closer to fungi. Also, it is better known and easy to manipulate. They are also already used to produce alcohols useful as an energy source (biofuels). There is a nice article of the journal nature about this. I can link it to you if you are interested.

Imho, a good fungus to use would be Phanerochaete chrysosporium  since his genome is already sequenced. You would have to overexpress the desired traits for bioremediation. However, i'm sure such strains already exists in the databases, you just have to search for them.

If not, Pleurotus ostreatus is also a good choice. His genome have been sequenced aswell.



Is that for bioremediation or is it for increasing suger integration


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