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Offlinecheech_wizard
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Registered: 03/26/03
Posts: 3
Last seen: 11 years, 1 month
slow mycelial growth
    #1786026 - 08/06/03 08:03 PM (13 years, 10 months ago)

what am i doing wrong- I have used the pre-steralized bag method for several strains. one of my bags seems to be only moderately heatlthy and I have tried varying temperature (aprrox. 84degrees), adding moisture over several months- there is some growth and no contamination. Is it possibe that the mycelium has been killed in this stage- or is it suspended in growth?


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OfflineMycoGlowFlow
between the walls
Male

Registered: 05/20/01
Posts: 1,102
Loc: michigan
Last seen: 8 years, 11 months
Re: slow mycelial growth [Re: cheech_wizard]
    #1786037 - 08/06/03 08:07 PM (13 years, 10 months ago)

maybe the bag was pre pc'd for too long by accident


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Edited by MycoGlowFlow (08/06/03 08:08 PM)


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OfflineMsPacMan
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Registered: 10/05/02
Posts: 1,054
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Re: slow mycelial growth [Re: MycoGlowFlow]
    #1786042 - 08/06/03 08:09 PM (13 years, 10 months ago)

imho, start with jars


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OfflineMycoGlowFlow
between the walls
Male

Registered: 05/20/01
Posts: 1,102
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Re: slow mycelial growth [Re: cheech_wizard]
    #1786047 - 08/06/03 08:09 PM (13 years, 10 months ago)

were the bags all innoculated from the same syringe/print?


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(the key-strokes that have completed everything ever typed on this user name have been a random coincidence, except for the words in this notation)


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Offlinecheech_wizard
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Registered: 03/26/03
Posts: 3
Last seen: 11 years, 1 month
Re: slow mycelial growth [Re: MycoGlowFlow]
    #1786066 - 08/06/03 08:16 PM (13 years, 10 months ago)

nope-
they were of different type - is it possible the spores are inefective after several months?


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Offlinemr_minds_eye
Disposable Wage Whore
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Registered: 01/22/02
Posts: 1,948
Loc: Samsara
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Re: slow mycelial growth [Re: cheech_wizard]
    #1786071 - 08/06/03 08:18 PM (13 years, 10 months ago)

Aging definitely is going to have a noticeable effect.



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Our quest for discovery fuels our creativity in all fields, not just science. If we reached the end of the line, the human spirit would shrivel and die. But I don't think we will ever stand still: we shall increase in complexity, if not in depth, and shall always be the center on an expanding horizon of possibilities.
-Stephen Hawking


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OfflineMycoGlowFlow
between the walls
Male

Registered: 05/20/01
Posts: 1,102
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Last seen: 8 years, 11 months
Re: slow mycelial growth [Re: cheech_wizard]
    #1786099 - 08/06/03 08:24 PM (13 years, 10 months ago)

my expert guess is that the bag was bad, not the spores. because u didnt prepare the substrate urself, and im guessing u didnt make the syringe either, its pretty hard to exactly pinpoint ur problem..


my guess is a bad bag, old or not made with the right ratio of grain to water... u can moisten the bag afterwards, but u have to pc the substrate in the presence of moisture to actually get it to draw the water in... which isnt really an option after innoculation

bad bag


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(the key-strokes that have completed everything ever typed on this user name have been a random coincidence, except for the words in this notation)


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