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HIGHIMTHENEWGUY


Registered: 05/15/08
Posts: 162
Loc: Wonderland
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Pan Cambogenisis on agar INPUT NEEDED!!
#17833993 - 02/20/13 02:35 AM (11 years, 2 months ago) |
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been over a week, probably 8-9 days. Took a multispore syringe to agar, no sign of growth yet. I only made 1 plate. It is MEA agar, which as far as i know should be suitable. Does Pan Cambogenisis just not grow well on agar? Is it difficult to make isolates of Pan Cambogenisis? I just wonder if I am going about this all wrong, if instead of working it on agar if i should be making manure jars and innoculating or making more plates and try obtaining growth and isolating. Need some opinions from fellow knowledgeable shroomerites
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Fruitbuddy
Mycophilic Cultivator


Registered: 09/01/10
Posts: 214
Last seen: 7 years, 6 months
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: HIGHIMTHENEWGUY]
#17834231 - 02/20/13 05:12 AM (11 years, 2 months ago) |
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how old are the spores? if you have viable spores, Pan. cambodgiensis should be no problem to germinate on agar.
just have some more patience.
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Spicy
Symbiont


Registered: 01/22/13
Posts: 208
Last seen: 5 months, 6 days
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: Fruitbuddy]
#17834730 - 02/20/13 09:10 AM (11 years, 2 months ago) |
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Make more than one plate, if you get unlucky one drop of solution might not contain spores. Pan syringes can be light on spores, if you can see them or not, so do a few plates and make sure you get spores on there.
Spicy
-------------------- Everything has beauty, not everyone sees it. Perfection is subjective!
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HIGHIMTHENEWGUY


Registered: 05/15/08
Posts: 162
Loc: Wonderland
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: Fruitbuddy]
#17835154 - 02/20/13 10:54 AM (11 years, 2 months ago) |
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That's the thing fruitbody, It is a brand new spore syringe from a shroomery vendor. It has been at least 9 days, maybe 2 weeks now. I think maybe I just didnt get any spores when i put a drop on the agar plate.....Thing is this is my only syringe of Pan Cambogenisis so I gotta make it work. Agar always seems like the first most viable option. Hell it can't be that light of a syringe, I can see the spores moving around. From what little is spoke of Pans, I hear the mycelium is faster than cubes. I'm baffled, maybe i just need to make more plates.....
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Fruitbuddy
Mycophilic Cultivator


Registered: 09/01/10
Posts: 214
Last seen: 7 years, 6 months
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: HIGHIMTHENEWGUY]
#17835714 - 02/20/13 12:37 PM (11 years, 2 months ago) |
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yes, as Spicy already said, make more than one plate.
you don't have to waste a lot, but don't be too frugal. 
sometimes it might happen that the hot needle after flaming kills some spores, and when you use only the first drop, there might not be enough viable spores in there. this should not be a major factor, if you aren't extremely sparing with the suspension, though.
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HIGHIMTHENEWGUY


Registered: 05/15/08
Posts: 162
Loc: Wonderland
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: Fruitbuddy]
#17836328 - 02/20/13 02:21 PM (11 years, 2 months ago) |
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Well, I looked at it again today and noticed a dime sized piece of mycelium. It is much more whispy looking than cube mycelium on agar. Will i even be able to create an isolate of Pan Cambogenisis? I hear pan mycelium grows fast so I guess I'll keep an eye on it.
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Nobitte
Student


Registered: 02/20/08
Posts: 493
Loc: Biosphere
Last seen: 6 years, 7 months
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: HIGHIMTHENEWGUY]
#17836493 - 02/20/13 02:52 PM (11 years, 2 months ago) |
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Ive found that straight MEA can also be hard to start spores on, if you continue to have trouble, try adding some milled wbs/milled wbs extract, Ive found increased vitality on that medium for some of my cultures that appear not to like MEA.
Milling the WBS just involves putting it through a coffee grinder or something similar till you have a powder and then either adding that or the filtered (put the grinds in a solution for a while and then put through a coffee filter, should be relatively clear) extract of that to your MEA mixture (maintaining the same water-agar ratio).
Im pretty sure its not my idea, but it works a charm, if anyone can tell me where Ive got it from, i would be most appreciative. Hope that helps xx
Sounds like you have Pan mycelia, you could probably create an isolate, but i tend to fruit MS and clone a desirable fruitbody before i start isolating, im not sure whether this is a terrible idea.
-------------------- First we must learn... Then... WE CAN TEACH
Edited by Nobitte (02/20/13 02:54 PM)
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Fruitbuddy
Mycophilic Cultivator


Registered: 09/01/10
Posts: 214
Last seen: 7 years, 6 months
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: Nobitte]
#17837648 - 02/20/13 06:29 PM (11 years, 2 months ago) |
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Quote:
Nobitte said: Ive found that straight MEA can also be hard to start spores on, if you continue to have trouble, try adding some milled wbs/milled wbs extract, Ive found increased vitality on that medium for some of my cultures that appear not to like MEA.
depends on the spores. i had no problem germinating spores on minimal molasses agar (after that step i would transfer to richer agar), but some spores can benefit from better nutrition. though, more nutrients can also help contaminations, so minimal agar can give you some more time, especially when working with wild samples. also, translucent agar helps with spotting contaminations, and i guess the wbs would make it more cloudy(?).
Quote:
Nobitte said: Sounds like you have Pan mycelia, you could probably create an isolate, but i tend to fruit MS and clone a desirable fruitbody before i start isolating, im not sure whether this is a terrible idea.
if you want to be sure, this is actually a good approach. this way you greatly increase the chance to get fruits, and then get your fruiting isolate. but personally, i would rather isolate the stronger growers and take the risk of getting a poor fruiter. that's just me, though.
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Spicy
Symbiont


Registered: 01/22/13
Posts: 208
Last seen: 5 months, 6 days
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: Fruitbuddy]
#17838670 - 02/20/13 09:39 PM (11 years, 2 months ago) |
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I've never seen a Pan isolate that didn't fruit well but MS clones are nice too!
Glad it only needed a few more days to show growth! Good luck on your transfers, it will be very fast.
Spicy
-------------------- Everything has beauty, not everyone sees it. Perfection is subjective!
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HIGHIMTHENEWGUY


Registered: 05/15/08
Posts: 162
Loc: Wonderland
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: Spicy]
#17838705 - 02/20/13 09:44 PM (11 years, 2 months ago) |
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I would prefer to make an isolate rather than clone a fruit. The only thing that has me a little hesitant is how whispy the mycelium is. I fear i will damage it. If others have had Pan isolates that makes me feel better like maybe it is possible lol.
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PaperTiger
Gnome
Registered: 11/04/03
Posts: 62
Last seen: 6 years, 3 months
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: HIGHIMTHENEWGUY]
#17839081 - 02/20/13 11:12 PM (11 years, 2 months ago) |
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Any chance you could post a picture of it? I recently had some Pan cyanescens germinate on MEA and the mycelium looked extremely light and fragile. Transferred to grain using agar-derived LC with the remnants of the plate after transfers were made about two weeks ago, still haven't seen any noticeable growth. By the way, it took 2-3 weeks for my samuiensis to germinate on MEA from a spore print. Guess its just the luck of the draw..
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HIGHIMTHENEWGUY


Registered: 05/15/08
Posts: 162
Loc: Wonderland
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: PaperTiger]
#17839532 - 02/21/13 01:53 AM (11 years, 2 months ago) |
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I will try and post a pic tomorrow for you PaperTiger.
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Spicy
Symbiont


Registered: 01/22/13
Posts: 208
Last seen: 5 months, 6 days
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: HIGHIMTHENEWGUY]
#17840714 - 02/21/13 10:59 AM (11 years, 2 months ago) |
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Give it time to grow out the mycelium will improve and be easier to transfer. It's definitely possible to isolate lol 
Spicy
-------------------- Everything has beauty, not everyone sees it. Perfection is subjective!
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HIGHIMTHENEWGUY


Registered: 05/15/08
Posts: 162
Loc: Wonderland
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: PaperTiger]
#17844635 - 02/21/13 09:56 PM (11 years, 2 months ago) |
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Not much to see since first signs of growth were yesterday evening but I took a pic last night for you PaperTiger as you asked.
Sorry for the crappy pic. This is a pretty decent camera im using is the sad part. Agar dishes are a pain in the ass to take photos of, but I also have never been known to be a great photographer
Edited by HIGHIMTHENEWGUY (02/21/13 09:58 PM)
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PaperTiger
Gnome
Registered: 11/04/03
Posts: 62
Last seen: 6 years, 3 months
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: HIGHIMTHENEWGUY]
#17846789 - 02/22/13 11:23 AM (11 years, 2 months ago) |
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Thanks High, I appreciate it. As far as I can tell, it looks like you're doing well. Way to go. I think I should probably start over with mine. Looks nothing like yours nor anyone elses. Keep us posted with any progress
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HIGHIMTHENEWGUY


Registered: 05/15/08
Posts: 162
Loc: Wonderland
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: PaperTiger]
#17894972 - 03/02/13 11:06 PM (11 years, 2 months ago) |
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Update, transferred healthiest growth to new dishes
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Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 6 months
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: HIGHIMTHENEWGUY]
#17936481 - 03/10/13 10:48 PM (11 years, 2 months ago) |
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Assuming you are using a digital camera, look for the little flower icon to turn on your macro mode. This will make your close-up shots a lot clearer. Taking photos of agar is all about the macro mode and getting the right angle with the light to go through your plate.
I'd love to see your progress! 
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HIGHIMTHENEWGUY


Registered: 05/15/08
Posts: 162
Loc: Wonderland
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Re: Pan Cambogenisis on agar INPUT NEEDED!! [Re: Primal Call]
#17936546 - 03/10/13 11:04 PM (11 years, 2 months ago) |
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Thank you for the tip Ryath, greatly appreciated! I will give an update on this, I was actually planning on another transfer. Will drop an update tomorrow
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HIGHIMTHENEWGUY


Registered: 05/15/08
Posts: 162
Loc: Wonderland
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LONG OVERDUE UPDATE!!!! After the 3rd plate I transferred a wedge to a grain jar. It is fully colonized, which I will g2g to more jars within the next couple days. I also continued upon my quest to obtain an isolate but that idea got screwed because I didn't know what I am supposed to be isolating towards, lost interest and forgot about the plate and ditched it. Since the mycelium looks so different than cubensis I had a hard time in my quest towards Pan isolation. The good news is that after I g2g I can prep my tubs. Then fruits, oh I can't wait to try these! I have never had the privledge to try anything else, so you could say I'm pretty hyped
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Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 6 months
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Pans, from what I hear, are super-abundant and mostly uniform in flushing. So isolation is not all that necessary. I'd still love to see and test this type of thing out, though,... at least a clone if not an isolate.
Never tried anything else? As in you haven't had any mushrooms before? If so, be careful. Most people say a single gram of Pans is pretty intense.
Let's see the pics!
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