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OfflineRogerRabbitM
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Re: your thoughts or suggestions on my laminar flow hood project [Re: God Stamet]
    #17926872 - 03/08/13 10:16 PM (11 years, 2 months ago)

If it's not too resistive you could use it for a prefilter but you'd be stuck using that size from now on if you built the prefilter housing to hold it.  You'll change the prefilter every 100 hours or so of operation.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinet3chnobily
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Re: your thoughts or suggestions on my laminar flow hood project [Re: RogerRabbit]
    #17928048 - 03/09/13 06:38 AM (11 years, 2 months ago)

as long as your blower can handle the combined static pressure

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OfflineGod Stamet
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Re: your thoughts or suggestions on my laminar flow hood project [Re: RogerRabbit]
    #17928060 - 03/09/13 06:45 AM (11 years, 2 months ago)

Quote:

RogerRabbit said:
If it's not too resistive you could use it for a prefilter but you'd be stuck using that size from now on if you built the prefilter housing to hold it.  You'll change the prefilter every 100 hours or so of operation.
RR




i was trying to figure out a way to swap it with a normal pre filter. if you're supposed to change them every 30 to 50 times you use the flow hood then i'd better just go with a simple cheap easy to find pre filter. especially because i have a cat!

thanks RR


--------------------
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just another mushroom capsule TEK

Edited by God Stamet (03/09/13 06:46 AM)

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Invisiblefastfred
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Re: your thoughts or suggestions on my laminar flow hood project [Re: RogerRabbit]
    #18065690 - 04/06/13 02:50 AM (11 years, 1 month ago)

Quote:

RogerRabbit said:
We've known for years that UV has no place in mycology.  You're not re-inventing the wheel, but rather using a square wheel that has already been disproved.  I own a commercial mushroom farm and know and deal with dozens of others in the business and not a single one of them uses UV.  Disregard bad advice from other noobs pretending to know what actual mycology labs do and use.
RR




Still haven't been in a real lab I see RR.  UV is standard in a lot of flow hoods and almost every sterile transfer room.

UV is so cheap and easy there's just no good reason not to use it.  Anyone interested can google UV bulbs.  Look for a standard size one and run it off a normal (proper wattage) ballast.

UV is no magic bullet, but it's very effective for surface sterilization.


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid

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OfflinePussyFart
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Re: your thoughts or suggestions on my laminar flow hood project [Re: fastfred]
    #18065877 - 04/06/13 05:11 AM (11 years, 1 month ago)


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Invisiblefastfred
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Re: your thoughts or suggestions on my laminar flow hood project [Re: PussyFart]
    #18067662 - 04/06/13 03:13 PM (11 years, 1 month ago)

UV burns are not fun.  These things are NOT tanning lamps, you won't get a tan from them just a nasty burn.

Most people I know only F-up once with UV.  Once you have a good burn and your eyes feel like sand the lesson is learned.  UV burning your eyeballs is not normally repeated.

Again, UV is for when you are NOT in the room.  But any plastic will block UV, so if you have a plexiglass front on your flow hood you can use one while working, it's just generally not done since there's not much point.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid

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OfflinePussyFart
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Re: your thoughts or suggestions on my laminar flow hood project [Re: fastfred]
    #18067729 - 04/06/13 03:27 PM (11 years, 1 month ago)

Quote:

fastfred said:
Again, UV is for when you are NOT in the room.  But any plastic will block UV,



"""A University lab employee received skin and eye burns while using an acrylic plastic shield for protection against UV. The lab did not realize that the shield had not been manufactured for this use and was not rated for protection against UV light. Please check your safety equipment to ensure that it is rated for the wavelength in use."""
http://www.ehs.washington.edu/rsononion/uvlight.shtm

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Invisiblefastfred
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Re: your thoughts or suggestions on my laminar flow hood project [Re: PussyFart]
    #18067887 - 04/06/13 04:08 PM (11 years, 1 month ago)

Acrylic/plexiglas is opaque to UV.  Doesn't really matter if it's "manufactured for this use" or "rated for protection against UV light".

That little low power light box didn't give anybody "skin and eye burns", unless they stared at it for an extended period of time without being behind that shield.

In any case, don't stare at UV light without adequate protection.  A decent thickness plexiglas and glasses should be considered the minimum protection for anything more than several minutes.

IME the 25 watt UV tubes are about twice as fast as the sun at burning you, and the burn is easily twice as unpleasant.  30 minutes can give you an unpleasant burn.  Even with eye protection you want to limit your exposure to less than 5-10 minutes a day if you are light skinned.

It's very easy to get burned if you use UV improperly, but very hard to have any risk if you do use it properly.

I should probably quit pitching UV since it seems that nobody believes me until they get that first burn, or else they're too much of a paranoid to use it in the first place.

One guy I helped out decided he liked the UV idea so much that he bought 8-10 UV tubes and had his damn room lit up like the sun with them.  I told him many times to turn the damn things off when he was in there, or limit his time AND use sunscreen and eyewear.  But, all excited, he told me one day he knew the UV was working great because it burned the hell out of him and his eyes felt like sandpaper for a handful of days after he ignored my warning.

There's no reason not to have UV in your setup.  Buy a cheap under cabinet light fixture ($10), hook up a properly rated ballast ($10-25), and slap in a UV tube ($12).  It's very cheap and easy if you go to someplace like 1000bulbs.com and get the properly sized tubes.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid

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OfflinePussyFart
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Re: your thoughts or suggestions on my laminar flow hood project [Re: fastfred]
    #18067931 - 04/06/13 04:22 PM (11 years, 1 month ago)

Quote:

fastfred said:
There's no reason not to have UV in your setup. 



Other than it is just not needed, because people have 99-100% success without it.

Shit, I don't even have a flow hood, all I use is a SAB/GB, and I have a 99-100% success rate.

So like was said above, if you have the money and resources, go for it, but you can have great success without it, meaning it is just not needed.

If the fact that it is just not needed is not a good enough reason for you, I fear no reason will be good enough.

Edited by PussyFart (04/06/13 04:23 PM)

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Invisiblefastfred
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Re: your thoughts or suggestions on my laminar flow hood project [Re: PussyFart]
    #18068150 - 04/06/13 05:17 PM (11 years, 1 month ago)

Pretty weak logic there.  I guess we should throw out all our tools and do everything with a toothpick, drinking glasses, and tinfoil.  You can have great success without anything else, so give up all that other useless crap.

If you don't see the point of $30 devices that keep all your surfaces and your air sterilized 24/7 so that they're always sterile and ready.... well fine.  But it's kind of annoying when a hypocrite keeps arguing about something they know little or nothing about.  Logically flawed and hypocritical arguments against a tech that is already standard practice across the scientific community is some pretty weak sauce.

If you have a sterile transfer room you really have no other option.  Either every lab tech that needs 5 minutes in the room has to spend half an hour wiping it down and still not get it half as clean or you plug in a UV light that's always on when the room is empty.  You can always spend a fortune on HEPA filters or use annoying and limiting things like glove boxes, but for the price and utility UV is far cheaper and easier.

The only real holdup in bringing wider use of UV to amateurs is getting people to learn how to do it cheap.  If you can use the internet and install a light fixture you can have cheap UV!


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid

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OfflinePussyFart
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Re: your thoughts or suggestions on my laminar flow hood project [Re: fastfred]
    #18068216 - 04/06/13 05:32 PM (11 years, 1 month ago)

Weak logic?

If it's not broke, don't fix it is not weak logic.

To each his own...I'll stick with my SAB, it cannot possibly work any better than it's working right now, nor would I want it to.

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Re: your thoughts or suggestions on my laminar flow hood project [Re: PussyFart]
    #18068731 - 04/06/13 07:35 PM (11 years, 1 month ago)

> If it's not broke, don't fix it is not weak logic.

Yeah, it's as weak as it gets, and I already explained why.

It's hypocritical since you're not following your own logic, and just plain wrong on it's face.  The model T worked fine, so we should all be driving model Ts, right?  Morse code over telegraph wires worked just fine for communication, so you're going to throw out your cell phone right?

You also just plain don't make any sense.  The use of UV in microbiology has been around far longer than any techniques you use, so the UV wasn't broke... why did you fix it?

That's the problem with using foolish little quips as a substitute for logic and analysis.  We're not down at the bar debating which beer is the best, so breaking out lines from "Farmer Joe's Famous Quotes" isn't going to get you anywhere.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid

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OfflinePussyFart
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Re: your thoughts or suggestions on my laminar flow hood project [Re: fastfred]
    #18068819 - 04/06/13 07:48 PM (11 years, 1 month ago)

Your comparing a model T to modern cars....makes no sense. The model T is not what I would call "perfecttion", but what car is perfect?

I will tell you what is perfect, 100% success, that is perfect.

If you are getting 99-100% success without a flow hood or UV lights, just with a SAB, how much is really left to improve on?

I really do not want this to become a heated argument, but saying UV lights will help, when someone is already getting the best success rate possible, makes no sense.

If a noob reads this thread, will he end up investing in a UV light? Me thinks not.

Because a $15 SAB will work just as good, with no further investments needed to have great success.

All that would be needed besides a SAB/Flowhood, is great sterile technique, and using a UV light will not fix that.

Where is the logic in buying a light that will not improve a damn thing?

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OfflineRoman08640
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Re: your thoughts or suggestions on my laminar flow hood project [Re: PussyFart]
    #18069342 - 04/06/13 09:25 PM (11 years, 1 month ago)

So a UV light in my sab. Got it Notahacker, got it :wink:
I'll spread the word

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Re: your thoughts or suggestions on my laminar flow hood project [Re: Roman08640]
    #18069592 - 04/06/13 10:28 PM (11 years, 1 month ago)

Hopefully you're not using SAB, it's a poor choice of media IME.

A UV light is to sterilize the surfaces and air, it really has nothing to do with your personal success rates.  If you only need to use the most ghetto methods to do the few basic techniques you use then great.  Others may live in areas with higher contam loads, want to do more advanced techniques, or simply want to reduce their efforts and consumption of chemicals.  To many people $12 for a UV bulb is not a lot of money.  Others may be smart enough to see that the time and chem savings will quickly pay that investment back.

This is advanced mycology, not ghetto teks for the cheap and lazy, so I don't see why you feel the need to rail on in your lost arguments.  I've been gone from this forum for a few years, so apologies if I have been too argumentative, but I have only your current flawed arguments to judge you by.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid

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OfflinePussyFart
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Re: your thoughts or suggestions on my laminar flow hood project [Re: fastfred]
    #18069703 - 04/06/13 10:56 PM (11 years, 1 month ago)

Call it ghetto if it makes you feel better, there are a lot of ghetto TCs around here then.

Call it italian fucking french dressing for all I care, success is success, I just choose to invest the least amount possible, to get there.

You call it ghetto, I call it economical common sense.

:peace:

Edited by PussyFart (04/06/13 10:59 PM)

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Invisiblefastfred
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Re: your thoughts or suggestions on my laminar flow hood project [Re: PussyFart]
    #18069770 - 04/06/13 11:16 PM (11 years, 1 month ago)

> You call it ghetto, I call it economical common sense.

I'm just mainly calling this poor logic.
How many $5 cans of Lysol does it take to equal a $30 UV lamp?

If you think it's overkill, then just say so and leave it at that.  That's a perfectly reasonable argument.  Everything else you've said just faulty reasoning.


-FF

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Re: your thoughts or suggestions on my laminar flow hood project [Re: PussyFart]
    #18069866 - 04/06/13 11:31 PM (11 years, 1 month ago)

Quote:

fastfred said:
I'm just mainly calling this poor logic.
How many $5 cans of Lysol does it take to equal a $30 UV lamp?

If you think it's overkill, then just say so and leave it at that.  That's a perfectly reasonable argument.  Everything else you've said just faulty reasoning.




I do not use lysol, that shit is expensive, I do however use 70% alcohol, soap and water.

My main area that I work in is my bedroom. Unfortunately this is where I spend 80% of my life, so running a UV when I am not doing mycology poses serious health risks to me.

The alcohol is to clean WHILE I am working, it does not get used when I am not working.

Now just because you have this UV light, this will not mean that lysol/alcohol is not needed, you just need less of it.

Unless you assume the UV light(which is off when you are working remember) is going to somehow sanitize your gloved hands, jars, and immediate work area, and keep it sanitized while you are working.

I have said it is overkill, it was the first thing I said in this thread other than quotes, and it has been said plenty of times, but only now are you willing to accept it.

Quote:

Notahacker420 said:
Other than it is just not needed, because people have 99-100% success without it.



^^^^

That means it is overkill.

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Re: your thoughts or suggestions on my laminar flow hood project [Re: fastfred]
    #18069887 - 04/06/13 11:34 PM (11 years, 1 month ago)

To make this thread more useful, here is my quick guide from memory on getting your UV going...

Google "t8 uv germicidal lamp".  Click on shopping, that should give you any number of sites.  I suggest the one that is the cheapest source, around $6.88.  You want one that is G15T8.  This should work in a F15T8 light fixture.

Go to your local MegaMart or hardware store.  Buy an 18" fluorescent light fixture for one 15W bulb. Under-Cabinet types will usually have a switch on the cord or fixture and be cheaper.  Strip light type will be a little more and probably not have a switch, but they will be metal (withstands/reflects UV better).  They are about $8 and $14, respectively.  Make sure to take any plastic cover or diffuser off.

Now you've got a 15W, 18" germicidal lamp for just under $15.00!

Remember you're not trying to replace all your good sterile technique, such as swabbing down surfaces with disinfectant.  But "LYSOL Professional Brand III Disinfectant" is $9.99 at my local MegaMart, and you can certainly reduce your disinfectant consumption significantly by letting UV do the work.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid

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Re: your thoughts or suggestions on my laminar flow hood project [Re: fastfred]
    #18070849 - 04/07/13 07:42 AM (11 years, 1 month ago)

Despite the bickering this thread contains some good info on UV.  Thanks Fastfred.  Won't be installing one in my flowhood (overkill) But I think they have real potential as sanitizers for humidibuckets.  Got any experience with sterilizing liquids/fog with UV?

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