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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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Offlinerohitabong
shroomtastic
Registered: 07/14/02
Posts: 120
Loc: DC
Last seen: 13 years, 4 months
myctopia's invitro double end casing...
    #1779845 - 08/05/03 12:21 AM (13 years, 5 months ago)

AFOAF is a newb and got crazy experimental and decided to innoculate 4 of 10 EQ jars with this mod on the PF Tek. he put about 1 tablespoon of dry verm on bottom of jars before adding the BRF/VERM/water mix like it says here: http://www.mycotopia.net/teks/invitrocase.html....

problem is the other 6 jars are colonizing fast, whereas the 4 experimental jars look all mushy and the mycelium looks thin and transparent....also, the mycellium is about 20% colonized, the normal 6 jars are about 50% colonized

apparently myctopia says this experimental method makes colonization easier and faster.....what gives, ain't happening for my friend. GImme some input, anyone ever tried this....when does he junk these 4 jars, last thing he need is contams messing up the other beauties....

sorry for the rambling...last thing is that the myctopia method claims that inverting jars is risky....my friend just inverted his jars thinking it would knock out CO2...people had recommended it on this board.

here is the link, please send feedback my friend needs expert advice!


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In the Time of Chimpanzees I was a Monkey.


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InvisibleMagashM
Da Bud Guru
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Registered: 07/25/02
Posts: 5,875
Loc: Near Hilo
Trusted Cultivator
Re: myctopia's invitro double end casing... [Re: rohitabong]
    #1779861 - 08/05/03 12:28 AM (13 years, 5 months ago)

mycelium looks thin and transparent-----That sounds like cob web. Keep your eye on those jars.


--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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Offlinerohitabong
shroomtastic
Registered: 07/14/02
Posts: 120
Loc: DC
Last seen: 13 years, 4 months
Re: myctopia's invitro double end casing... [Re: Magash]
    #1779868 - 08/05/03 12:31 AM (13 years, 5 months ago)

well the 6 jars look like thick white according to my friend, whereas the other 4 are more whispy and hair like, thin if you will

magash u know you shit, any opinion on the tek?
what about inverting jars, is this bad? my friend did it anyway but doesn't see how air can get through the holes on the lids


--------------------
In the Time of Chimpanzees I was a Monkey.


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Offlinerohitabong
shroomtastic
Registered: 07/14/02
Posts: 120
Loc: DC
Last seen: 13 years, 4 months
Re: myctopia's invitro double end casing... [Re: rohitabong]
    #1779870 - 08/05/03 12:31 AM (13 years, 5 months ago)

haha magash i didn't mean that you are shit, i meant you know your shit.....oops


--------------------
In the Time of Chimpanzees I was a Monkey.


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InvisibleGestaltAssault
Sam Kohui (c)

Registered: 05/25/03
Posts: 420
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Re: myctopia's invitro double end casing... [Re: rohitabong]
    #1779911 - 08/05/03 12:45 AM (13 years, 5 months ago)

are you sure the spore solution didn't get soaked up by the casing layer?


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i dont cultivate...i just post pictures, spread knowledge, and give advice
my brain's illegal because it contains DMT in the pineal gland, and so does your brain and everyone elses
the name's GestaltAssault not Get Us A Slut


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Offlinerohitabong
shroomtastic
Registered: 07/14/02
Posts: 120
Loc: DC
Last seen: 13 years, 4 months
Re: myctopia's invitro double end casing... [Re: GestaltAssault]
    #1779928 - 08/05/03 12:49 AM (13 years, 5 months ago)

he has no clue...as long as he still sees that whispy white shit he will continue, but he's pretty sure those might take quite awhile....ah well

no let's talk inverted jars where the lid has holes drilled in it....is this bad to cover them when inverted and not let O2 to get into/CO2 to get out of


--------------------
In the Time of Chimpanzees I was a Monkey.


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InvisibleMagashM
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Re: myctopia's invitro double end casing... [Re: rohitabong]
    #1779937 - 08/05/03 12:52 AM (13 years, 5 months ago)

It's a good tek. The only difference is that most that try it seem to think it speeds things up.

Turning the jars over is fine. I put a layer of towls between the jars when they are turned over so the bottom of one jar doesn't plug the holes on the other jar on top of it.

I didn't do invitro but it tells how I do the jars.

Oh yeah I'm retired, I forgot for a sec. :grin:

Grow Log
Tek : PF
Strain : South American [SA]
Spores : Came from homemade prints. 36 total jars to be injected.
Substrate :Brown Rice Flour [BRF] and Vermiculite [Verm]
---------------------------------------------------------------------------------------------------
10:15 PM 3/4/03 : Project is started. Jar prep is just putting small holes in the lids for the spore syringe and then taping them closed until inoculation. Tape used is the standard black electrical tape. First batch of jars is mixed. The mix used is 2 parts verm, 1 part water, and 1 part brf.  [ This mix is the max fruiting formula by PF.] Mix is put into 1/2 pint canning jars lightly, not packed in, and left it airy. Put substrate in until it reaches the threads of the jar, then add dry verm the rest of the way.
Jars are PCd at 10psi or 240f for a period of 45 minutes. Then jars were left to sit overnight to cool.
 

8:39 PM 3/5/2003 : First batch of jars injected. (24 jars)

10:04 AM 3/6/2003 : Incubator is running to low 77F. Going to turn it up today.


5:45 PM 3/6/2003 : Second batch of jars is in the PC will be injected tomorrow. Turned up incubator runs at 83F. 86F is the best and if the incubator is 83F and myc growing in the jars is suppose to create a little heat I figure this should work out well.

1:11 PM 3/7/2003 : Second batch of jars is done. (12 jars)

12:10 PM 3/9/2003 : Jars are doing good, no signs of contams


2:54 AM 3/19/2003 : Tape removed from the holes today and coffee filters put over the top of the jars for added protection. Holes were opened so the CO2 in the jars could escape. I gave 12 jars to a friend who kept fucking up. 24 jar?s about 75%done.


12:13 PM 3/22/2003 : Well it's Saturday and the jars will probably be done by the 25th. So it was 36 jars. 12 were given to a friend, 24 done by Tuesday.

10:26 PM 3/23/2003 : 15 jars were done enough to put into dunk. I dunk and cold shock before birthing and after every flush.  The other 9 jars have a spot the size of a pea that has to be covered so I'll do those on the 25th.
 

7:17 PM 3/24/2003 : 15 jars put into the pod. Put in and put a layer of moist verm on top. With geolite I just put the cakes on it. With some other chambers I put the cake on a lid filled with verm. This is done to give the shrooms on the bottom and the top of the cake some added moisture.
The other 9 jars just finished so I'm going to give them a couple more days to make sure the middle of the cake is done.


4:57 PM 3/27/2003 : 5 More cakes birthed today, 4 left going to do them tomorrow or  3/29/2003

3:58 PM 3/29/2003 : Well I lost another cake. My wife's little 1 cake pod made from a 2 liter and a small fish pump and a little geolite used up her last cake so I gave her a SA to grow. The last 3 are in the cold shock dunk.
So the total is 36 jars done all colonized with no contams. 12 given to a friend, 1 to the wife, 23 mine

12:08 PM 3/30/2003 : Last of jars put in 

12:02 PM 3/31/2003 : The first 15 cakes are pinning nicely and some have shrooms coming from the bottom.


11:01 AM 4/3/2003 : Things look to be going well.


1:02 AM 4/4/2003 : Took the first flush from four of the cakes. More will be done later today.


5/8/2003 : Total 23 cakes 164 grams dried. 


--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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