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 FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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 Frank's Proper Cloning Tek  37
#17784996 - 02/11/13 06:34 PM (11 years, 2 months ago) |
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This is part of my series on how I get things done. I hope it helps!
The easiest way to get a good set of genetics is to clone an ideal looking fruit or cluster from a multispore grow. This process is very simple, and the way I do it is essentially the same way that RR does it.
Some people will try to clone into LC or using a needle, etc. This will almost always fail. Make sure to use agar. Since fruiting is done in open air, you can always assume the fruit is covered in contaminants. This is doubly true for cloning wild mushrooms.
Even though you'll be taking a cut of virgin mycelium that has never been exposed to open air, it is very common to need to transfer your mycelium away from contaminants on the first dish.
Since a flowhood can potentially blow contaminants around from the fruit you are cloning, I recommend working in a still air box.
Start by wearing tyvek sleeves and disposable gloves. Make sure to work quickly and carefully inside the still air box. Use good sterile technique.
Flame sterilize your scalpel and set it down in the still air box where it won't be disturbed. I place it on my receiving petri dish. Do not set it on the floor of your SAB.
Take the mushroom (this is a golden oyster) and pinch it at the base. Rip it down the middle. Do not cut into the stem with your scalpel or you will force contaminants down into it.
   
Holding part of the fruit with one hand, use your scalpel to gently scrape at the center of the stem, near the base. Try to get a small piece on to the blade. As RR says, a scraping motion will be easier than trying to slice a specific piece.
Once you have a small piece of tissue on the end of your scalpel, transfer it to the agar. It may take a bit of effort to get it off the blade 
 
Take as many pieces of tissue as you want to clone. You can flame sterilize between each cut, or just flame sterilize between each petri dish or fruit. I use 3 section dishes so I flame sterilize every three cuts.
That's all there is to it. Wrap the petri dish and let it colonize. Transfer healthy mycelium away from any contaminants as soon as they appear!
Some fruits will give you an isolate right off the bat, but a lot of mushrooms are actually composites of multiple genetics. However, I have seen TCs say that the original clone sectors perform better together than as an isolate of each. So it's up to you if you decide to isolate the clone further 
Edited by FrankHorrigan (03/03/13 10:17 PM)
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 cherry_darling
i smell horseshit!
Registered: 12/08/12
Posts: 589
Loc: Planet Terror (ohio)
Last seen: 10 years, 9 months
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>bookmarked<
thanks for writing this up, you have been of infinite help to me personally, as I'm sure many others! 
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JoeMama1992
Asleep at the Wheel
Registered: 12/27/11
Posts: 901
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*Bookmarked*
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Deadkndys420
The Psychedelic Messiah
Registered: 05/08/12
Posts: 4,405
Loc: In the land of ooo
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 Re: Frank's Proper Cloning Tek [Re: JoeMama1992]
#17785213 - 02/11/13 07:06 PM (11 years, 2 months ago) |
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Quote:
cherry_darling said:
>bookmarked<
Quote:
JoeMama1992 said:
*Bookmarked*
--------------------
Almond Flour said:
Thats right. I take it up the ass on a daily. It feels great to finally let this out 
 
How to use PGP encryption
The Stoner's Cookbook
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Re: Frank's Proper Cloning Tek [Re: Deadkndys420] 1
#17788603 - 02/12/13 11:01 AM (11 years, 2 months ago) |
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I thought the forums could use a good cloning write up.
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wire5
Hippie Born too Late
Registered: 04/05/11
Posts: 1,566
Loc: Rollin' on the River
Last seen: 8 years, 1 month
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Pirax
Self Employed Pharmacist
Registered: 11/26/12
Posts: 388
Loc: Boregon
Last seen: 7 years, 8 months
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Re: Frank's Proper Cloning Tek [Re: wire5]
#17788648 - 02/12/13 11:09 AM (11 years, 2 months ago) |
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Nice tek, you posted this the day my caps opened up and my MEA arrived, nice timing
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TheCyndicate
Conglomerate
Registered: 10/16/11
Posts: 1,195
Loc: Outer Haven
Last seen: 5 years, 11 months
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Re: Frank's Proper Cloning Tek [Re: Pirax]
#17788821 - 02/12/13 11:44 AM (11 years, 2 months ago) |
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You know what I'm gonna say frank!
Cyn
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HypnotoadCroaked
Retired, but will check MSGs
Registered: 01/05/13
Posts: 1,168
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THANK YOU FRANK. I have to say that the awesomest thing about this forum, is that whenever I have a question, or are about to try something risky, someone posts the correct method a few days prior.
This is a great writeup, and I will CERTAINLY be using it in the very near future. I can assume it would work with Doc34's cheap-skate agar tek?
http://www.shroomery.org/8514/Agar-substitute
Thanks Frank!
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gizmo1
Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM 
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I have been taking my tissue samples straight to grain risky I know but hasn't failed me yet(knock on wood).
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6 hole Mini Monos
Edited by gizmo1 (03/01/13 08:51 PM)
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b plus
Registered: 10/13/12
Posts: 928
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Re: Frank's Proper Cloning Tek [Re: gizmo1]
#17888667 - 03/01/13 08:34 PM (11 years, 1 month ago) |
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gizmo- try this:
Take the tissue sample, and toss into a newly opened ziploc bag. Leave for 2-3 weeks. Don't worry about holes for GE or anything. Acutally compress almost all of the air out of the bag so only a very small amount is left. Also make sure the sample is a little smaller than the size or a pea or pencil eraser or I find that it tends to dry out. After a week or two, the tissue sample is covered in fluffy white myc. Almost like a petri, without the agar, or the dish... just a bag.. I've had pieces that clearly show signs of bacterial contam, but I very rarely have contamed peices. Most of the time they just dry out too quickly before myceium begins to grow.
Anyways while in a SAB, transfer it from the bag into the grain jar... you know the drill
Edited by b plus (03/01/13 08:35 PM)
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Mycospore
Registered: 09/24/11
Posts: 76
Last seen: 14 days, 2 hours
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Re: Frank's Proper Cloning Tek [Re: b plus]
#17889022 - 03/01/13 09:17 PM (11 years, 1 month ago) |
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Re: Frank's Proper Cloning Tek [Re: b plus]
#17896744 - 03/03/13 11:04 AM (11 years, 1 month ago) |
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Quote:
TheCyndicate said:
You know what I'm gonna say frank!
Cyn
Oh I do! 
Where ya been lately, Cyn?
Quote:
Whippy said:
THANK YOU FRANK. I have to say that the awesomest thing about this forum, is that whenever I have a question, or are about to try something risky, someone posts the correct method a few days prior.
This is a great writeup, and I will CERTAINLY be using it in the very near future. I can assume it would work with Doc34's cheap-skate agar tek?
http://www.shroomery.org/8514/Agar-substitute
Thanks Frank!
I'm glad I could help!
I am sure it could be used with agar subs, but I would just grab a sleeve of petris and some malt extract agar and try it for real
Quote:
b plus said:
try this
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tripdawg420
low life with no life
Registered: 02/02/09
Posts: 7,076
Loc: illinois
Last seen: 9 hours, 36 minutes
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i see a TC tag in your future  you pretty much summed it up
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TheCyndicate
Conglomerate
Registered: 10/16/11
Posts: 1,195
Loc: Outer Haven
Last seen: 5 years, 11 months
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Re: Frank's Proper Cloning Tek [Re: tripdawg420]
#17898326 - 03/03/13 04:31 PM (11 years, 1 month ago) |
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Frank.
Taking a break man . I love our hobby with a passion! !. But it was consuming me and all of my free time. Desided to spend a little more time with my lovely wife and little ones . And consntrate on whats important. . Dont worry I'm still here . Not going anywhere . Just chillin out for a while!!
Cyn
Edited by TheCyndicate (03/06/13 06:58 PM)
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Quote:
tripdawg420 said:
i see a TC tag in your future you pretty much summed it up
I'm just glad I can help out the community that taught me almost everything I know!
Quote:
TheCyndicate said:
Frank.
Taking a break man . I love your hobby with a passion! !. But it was consuming me and all of my free time. Desided to spend a little more time with my lovely wife and little ones . And consntrate on whats important. . Dont worry I'm still here . Not going anywhere . Just chillin out for a while!!
Cyn
Good to hear things are well in your world, man.
It is a time consuming hobby. People ask how much I actually work when I'm sitting at home and I tell them "6-8 hour days."
They think I'm lying, but I'm not 
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PussyFart
Retired Cultivation Extrodinaire
Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 3 months, 2 days
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Yes, but I try think of it as productive entertainment, as opposed to work, but after a while, it becomes work, then a pain, then an obsessive passion where it's only driving force is you, lol.
--------------------
  
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
 *** *** AFGHAN KUSH GROW LOG *** ***
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Re: Frank's Proper Cloning Tek [Re: PussyFart]
#17900682 - 03/04/13 12:11 AM (11 years, 1 month ago) |
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Quote:
Notahacker420 said:
Yes, but I try think of it as productive entertainment, as opposed to work, but after a while, it becomes work, then a pain, then an obsessive passion where it's only driving force is you, lol.
Agreed. I love every second of it.
They call it "work," I just let them think of it that way
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annnon
KillerClown
Registered: 05/12/13
Posts: 86
Loc: Between Heaven/Hell
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Hay Frank, can you please explain to me why you would grab your scraping of tissue from the base and not the middle or head? Also
I have tried a tissue to grain and it was painfully slow so instead of using one scraping I used ten per jar. The myc popped up in many areas quickly, not in every jar though and that is why I am asking what I am.
Thanks mucho in advance my good man!
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itsaconspiracy
governments worst nightmare
Registered: 03/26/13
Posts: 2,807
Loc: florida
Last seen: 2 years, 7 months
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Re: Frank's Proper Cloning Tek [Re: annnon]
#18261403 - 05/14/13 12:10 AM (10 years, 10 months ago) |
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 i like it and why didn't you use a Psilocybe cubensis
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("i'm not a criminal i just like stuff thats not mine")
"bender"
  
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