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 FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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 Frank's Proper Cloning Tek  37
#17784996 - 02/11/13 06:34 PM (11 years, 2 months ago) |
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This is part of my series on how I get things done. I hope it helps!
The easiest way to get a good set of genetics is to clone an ideal looking fruit or cluster from a multispore grow. This process is very simple, and the way I do it is essentially the same way that RR does it.
Some people will try to clone into LC or using a needle, etc. This will almost always fail. Make sure to use agar. Since fruiting is done in open air, you can always assume the fruit is covered in contaminants. This is doubly true for cloning wild mushrooms.
Even though you'll be taking a cut of virgin mycelium that has never been exposed to open air, it is very common to need to transfer your mycelium away from contaminants on the first dish.
Since a flowhood can potentially blow contaminants around from the fruit you are cloning, I recommend working in a still air box.
Start by wearing tyvek sleeves and disposable gloves. Make sure to work quickly and carefully inside the still air box. Use good sterile technique.
Flame sterilize your scalpel and set it down in the still air box where it won't be disturbed. I place it on my receiving petri dish. Do not set it on the floor of your SAB.
Take the mushroom (this is a golden oyster) and pinch it at the base. Rip it down the middle. Do not cut into the stem with your scalpel or you will force contaminants down into it.
   
Holding part of the fruit with one hand, use your scalpel to gently scrape at the center of the stem, near the base. Try to get a small piece on to the blade. As RR says, a scraping motion will be easier than trying to slice a specific piece.
Once you have a small piece of tissue on the end of your scalpel, transfer it to the agar. It may take a bit of effort to get it off the blade 
 
Take as many pieces of tissue as you want to clone. You can flame sterilize between each cut, or just flame sterilize between each petri dish or fruit. I use 3 section dishes so I flame sterilize every three cuts.
That's all there is to it. Wrap the petri dish and let it colonize. Transfer healthy mycelium away from any contaminants as soon as they appear!
Some fruits will give you an isolate right off the bat, but a lot of mushrooms are actually composites of multiple genetics. However, I have seen TCs say that the original clone sectors perform better together than as an isolate of each. So it's up to you if you decide to isolate the clone further 
Edited by FrankHorrigan (03/03/13 10:17 PM)
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 cherry_darling
i smell horseshit!
Registered: 12/08/12
Posts: 589
Loc: Planet Terror (ohio)
Last seen: 10 years, 9 months
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>bookmarked<
thanks for writing this up, you have been of infinite help to me personally, as I'm sure many others! 
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JoeMama1992
Asleep at the Wheel
Registered: 12/27/11
Posts: 901
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*Bookmarked*
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Deadkndys420
The Psychedelic Messiah
Registered: 05/08/12
Posts: 4,405
Loc: In the land of ooo
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 Re: Frank's Proper Cloning Tek [Re: JoeMama1992]
#17785213 - 02/11/13 07:06 PM (11 years, 2 months ago) |
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Quote:
cherry_darling said:
>bookmarked<
Quote:
JoeMama1992 said:
*Bookmarked*
--------------------
Almond Flour said:
Thats right. I take it up the ass on a daily. It feels great to finally let this out 
 
How to use PGP encryption
The Stoner's Cookbook
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Re: Frank's Proper Cloning Tek [Re: Deadkndys420] 1
#17788603 - 02/12/13 11:01 AM (11 years, 2 months ago) |
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I thought the forums could use a good cloning write up.
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wire5
Hippie Born too Late
Registered: 04/05/11
Posts: 1,566
Loc: Rollin' on the River
Last seen: 8 years, 1 month
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Pirax
Self Employed Pharmacist
Registered: 11/26/12
Posts: 388
Loc: Boregon
Last seen: 7 years, 8 months
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Re: Frank's Proper Cloning Tek [Re: wire5]
#17788648 - 02/12/13 11:09 AM (11 years, 2 months ago) |
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Nice tek, you posted this the day my caps opened up and my MEA arrived, nice timing
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TheCyndicate
Conglomerate
Registered: 10/16/11
Posts: 1,195
Loc: Outer Haven
Last seen: 5 years, 11 months
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Re: Frank's Proper Cloning Tek [Re: Pirax]
#17788821 - 02/12/13 11:44 AM (11 years, 2 months ago) |
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You know what I'm gonna say frank!
Cyn
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HypnotoadCroaked
Retired, but will check MSGs
Registered: 01/05/13
Posts: 1,168
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THANK YOU FRANK. I have to say that the awesomest thing about this forum, is that whenever I have a question, or are about to try something risky, someone posts the correct method a few days prior.
This is a great writeup, and I will CERTAINLY be using it in the very near future. I can assume it would work with Doc34's cheap-skate agar tek?
http://www.shroomery.org/8514/Agar-substitute
Thanks Frank!
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gizmo1
Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM 
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I have been taking my tissue samples straight to grain risky I know but hasn't failed me yet(knock on wood).
--------------------
Trade List
🖕🖕🖕
6 hole Mini Monos
Edited by gizmo1 (03/01/13 08:51 PM)
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b plus
Registered: 10/13/12
Posts: 928
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Re: Frank's Proper Cloning Tek [Re: gizmo1]
#17888667 - 03/01/13 08:34 PM (11 years, 1 month ago) |
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gizmo- try this:
Take the tissue sample, and toss into a newly opened ziploc bag. Leave for 2-3 weeks. Don't worry about holes for GE or anything. Acutally compress almost all of the air out of the bag so only a very small amount is left. Also make sure the sample is a little smaller than the size or a pea or pencil eraser or I find that it tends to dry out. After a week or two, the tissue sample is covered in fluffy white myc. Almost like a petri, without the agar, or the dish... just a bag.. I've had pieces that clearly show signs of bacterial contam, but I very rarely have contamed peices. Most of the time they just dry out too quickly before myceium begins to grow.
Anyways while in a SAB, transfer it from the bag into the grain jar... you know the drill
Edited by b plus (03/01/13 08:35 PM)
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Mycospore
Registered: 09/24/11
Posts: 76
Last seen: 14 days, 3 hours
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Re: Frank's Proper Cloning Tek [Re: b plus]
#17889022 - 03/01/13 09:17 PM (11 years, 1 month ago) |
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Re: Frank's Proper Cloning Tek [Re: b plus]
#17896744 - 03/03/13 11:04 AM (11 years, 1 month ago) |
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Quote:
TheCyndicate said:
You know what I'm gonna say frank!
Cyn
Oh I do! 
Where ya been lately, Cyn?
Quote:
Whippy said:
THANK YOU FRANK. I have to say that the awesomest thing about this forum, is that whenever I have a question, or are about to try something risky, someone posts the correct method a few days prior.
This is a great writeup, and I will CERTAINLY be using it in the very near future. I can assume it would work with Doc34's cheap-skate agar tek?
http://www.shroomery.org/8514/Agar-substitute
Thanks Frank!
I'm glad I could help!
I am sure it could be used with agar subs, but I would just grab a sleeve of petris and some malt extract agar and try it for real
Quote:
b plus said:
try this
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tripdawg420
low life with no life
Registered: 02/02/09
Posts: 7,076
Loc: illinois
Last seen: 10 hours, 59 minutes
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i see a TC tag in your future  you pretty much summed it up
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TheCyndicate
Conglomerate
Registered: 10/16/11
Posts: 1,195
Loc: Outer Haven
Last seen: 5 years, 11 months
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Re: Frank's Proper Cloning Tek [Re: tripdawg420]
#17898326 - 03/03/13 04:31 PM (11 years, 1 month ago) |
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Frank.
Taking a break man . I love our hobby with a passion! !. But it was consuming me and all of my free time. Desided to spend a little more time with my lovely wife and little ones . And consntrate on whats important. . Dont worry I'm still here . Not going anywhere . Just chillin out for a while!!
Cyn
Edited by TheCyndicate (03/06/13 06:58 PM)
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Quote:
tripdawg420 said:
i see a TC tag in your future you pretty much summed it up
I'm just glad I can help out the community that taught me almost everything I know!
Quote:
TheCyndicate said:
Frank.
Taking a break man . I love your hobby with a passion! !. But it was consuming me and all of my free time. Desided to spend a little more time with my lovely wife and little ones . And consntrate on whats important. . Dont worry I'm still here . Not going anywhere . Just chillin out for a while!!
Cyn
Good to hear things are well in your world, man.
It is a time consuming hobby. People ask how much I actually work when I'm sitting at home and I tell them "6-8 hour days."
They think I'm lying, but I'm not 
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PussyFart
Retired Cultivation Extrodinaire
Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 3 months, 2 days
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Yes, but I try think of it as productive entertainment, as opposed to work, but after a while, it becomes work, then a pain, then an obsessive passion where it's only driving force is you, lol.
--------------------
  
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
 *** *** AFGHAN KUSH GROW LOG *** ***
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Re: Frank's Proper Cloning Tek [Re: PussyFart]
#17900682 - 03/04/13 12:11 AM (11 years, 1 month ago) |
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Quote:
Notahacker420 said:
Yes, but I try think of it as productive entertainment, as opposed to work, but after a while, it becomes work, then a pain, then an obsessive passion where it's only driving force is you, lol.
Agreed. I love every second of it.
They call it "work," I just let them think of it that way
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annnon
KillerClown
Registered: 05/12/13
Posts: 86
Loc: Between Heaven/Hell
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Hay Frank, can you please explain to me why you would grab your scraping of tissue from the base and not the middle or head? Also
I have tried a tissue to grain and it was painfully slow so instead of using one scraping I used ten per jar. The myc popped up in many areas quickly, not in every jar though and that is why I am asking what I am.
Thanks mucho in advance my good man!
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itsaconspiracy
governments worst nightmare
Registered: 03/26/13
Posts: 2,807
Loc: florida
Last seen: 2 years, 7 months
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Re: Frank's Proper Cloning Tek [Re: annnon]
#18261403 - 05/14/13 12:10 AM (10 years, 10 months ago) |
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 i like it and why didn't you use a Psilocybe cubensis
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("i'm not a criminal i just like stuff thats not mine")
"bender"
  
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lovedruid
viva forever!
Registered: 05/05/13
Posts: 156
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Re: Frank's Proper Cloning Tek [Re: annnon]
#18261410 - 05/14/13 12:11 AM (10 years, 10 months ago) |
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frank awesome guide this should be a sticky. i learned a lot from this i wanted to learn more about cloning. 5 star review.
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 "Power corrupts and absolute power corrupts absolutely" -Winston Churchill"
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PussyFart
Retired Cultivation Extrodinaire
Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 3 months, 2 days
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Re: Frank's Proper Cloning Tek [Re: annnon]
#18261419 - 05/14/13 12:13 AM (10 years, 10 months ago) |
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Quote:
annnon said:
Hay Frank, can you please explain to me why you would grab your scraping of tissue from the base and not the middle or head?
Because it is thought that this is where the most vigorous mycellium is.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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fungitarian
oliver hart
Registered: 04/08/13
Posts: 890
Loc: the land of ooo
Last seen: 10 years, 6 months
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Re: Frank's Proper Cloning Tek [Re: JoeMama1992]
#18261420 - 05/14/13 12:13 AM (10 years, 10 months ago) |
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awesome, never saw this tek. thanks for the billionth time frank 
Quote:
JoeMama1992 said:
*Bookmarked*
--------------------
have a look at my grow.
"genius by day, junkie by night"
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Re: Frank's Proper Cloning Tek [Re: PussyFart]
#18261514 - 05/14/13 12:33 AM (10 years, 10 months ago) |
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Quote:
Notahacker420 said:
Quote:
annnon said:
Hay Frank, can you please explain to me why you would grab your scraping of tissue from the base and not the middle or head?
Because it is thought that this is where the most vigorous mycellium is.
I've tried each site with cubensis and I get the best performance from close to the base of the stem
The reason you want to use agar for this annon is so you can have a vigorous and clean culture on agar for inoculating the grains with. Going direct from tissue to grain is risky, high failure rate.
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Steve Ignorant
Registered: 03/03/13
Posts: 63
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bookmarked
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fastfred
Old Hand
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Quote:
You can flame sterilize between each cut, or just flame sterilize between each petri dish or fruit. I use 3 section dishes so I flame sterilize every three cuts.
You should really sterilize between every transfer. I'm guilty of doing once per jar with needles, since it's kind of an all or nothing, but it's a bad habit to get into.
Before working with wild specimens I cut a section of stem, stab with a dental pick, dip for about 5-10 sec in 1:10 bleach, then 5-10 sec in peroxide.
Dental picks and scalpels work the best for tools. Both are pretty cheap so I keep a lot on hand. Wrap them in tinfoil and PC before starting.
My myc. professor had a pretty good system that I use. Get a jar, beaker, or any suitable heavy container, fill with IPA, and keep your tools all tip down in it. Remove a tool and flame it to burn off the alcohol, use it, then return it to the tool jar. Each time you grab a new tool. Since they're sitting in IPA you don't have to really flame sterilize them much, just burn off the alcohol.
You can use methanol, ethanol, denatured alcohol, IPA, etc. (not bleach). IPA and alcohols are hard on the tools and will rust them out, so rinse them off and dry them afterwards.
Hope my tips help with your guide Frank. I learned a few tricks myself reading it. I had previously done too much cutting and foolery. The tear method sounds much quicker and easier. I was using way too many tedious cuts to get down to clean tissue. Thanks for writing this up and reminding me of better ways.
-FF
--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy
The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed
"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)
I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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fungitarian
oliver hart
Registered: 04/08/13
Posts: 890
Loc: the land of ooo
Last seen: 10 years, 6 months
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Re: Frank's Proper Cloning Tek [Re: fastfred]
#18262033 - 05/14/13 03:15 AM (10 years, 10 months ago) |
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Great addition! Frank, you should include this in the OP.
Anyone reading this will find fastfred's information extremely helpful I'm sure.
--------------------
have a look at my grow.
"genius by day, junkie by night"
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK 
Last seen: 4 years, 3 months
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Re: Frank's Proper Cloning Tek [Re: fungitarian]
#18262125 - 05/14/13 03:46 AM (10 years, 10 months ago) |
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Nice, simple and straight to the point. I will have to give this a try when i get some fruits.
Unfortunatly no petri dishes yet so i will risk it with some grain jars.
Just as a reference for other people about going straight to grain being a contam risk. I done this with a button mushroom, split it open and transfered a slice of tissue to a grain jar.
After about 4 days it appeared to start going towards the grain, but then a week later mold spores showed throughout the grain jar.
--------------------
PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek
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newera
Stranger
Registered: 03/05/13
Posts: 359
Last seen: 4 years, 3 months
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Re: Frank's Proper Cloning Tek [Re: veda_sticks]
#18262158 - 05/14/13 03:58 AM (10 years, 10 months ago) |
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i was hoping to use this tek to clone some of my pans provided i get them to grow, but after reading a little more on them it appears people don't bother to with pans, i guess there so small it would be a little more difficult? or is it just unneccesary?
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hostyle
Stranger
Registered: 03/02/13
Posts: 60
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Hey Frank,
Thank you for posting this, very informative.
You said: " at the center of the stem, near the base."
What are the benefits of cutting clone tissue from the base?
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hostyle
Stranger
Registered: 03/02/13
Posts: 60
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Re: Frank's Proper Cloning Tek [Re: newera]
#18534929 - 07/09/13 04:10 PM (10 years, 9 months ago) |
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I find it difficult handle and cut cloning material from robust edibles. I can't imagine it would be any easier cloning piddly ass pan's
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Chuckfinely
another round for me an my buddy
Registered: 06/27/13
Posts: 628
Last seen: 4 years, 5 months
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Re: Frank's Proper Cloning Tek [Re: veda_sticks]
#18534945 - 07/09/13 04:13 PM (10 years, 9 months ago) |
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Quote:
veda_sticks said:
Nice, simple and straight to the point. I will have to give this a try when i get some fruits.
Unfortunatly no petri dishes yet so i will risk it with some grain jars.
Just as a reference for other people about going straight to grain being a contam risk. I done this with a button mushroom, split it open and transfered a slice of tissue to a grain jar.
After about 4 days it appeared to start going towards the grain, but then a week later mold spores showed throughout the grain jar.
I've taken tissue and dropped it into LC's infront of my flow hood. Made 3 LC's and each one survived. I think I knocked up around 50 jars with that LC and never had an issue.
I tried dropping tissue onto grains before but out of 6 jars only one finished. Was too nervous to use that jar to g2g others, but I spawned it to mini-bulk in a shoebox and it fruited fine
That said I still prefer petri's when/if I need new genetics and I'm not too lazy to make and pour the agar
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vanislandshroomer
Innocent Bystander
Registered: 09/24/11
Posts: 573
Loc: Vancouver Island 
Last seen: 6 months, 2 days
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Re: Frank's Proper Cloning Tek [Re: Chuckfinely]
#18534992 - 07/09/13 04:23 PM (10 years, 9 months ago) |
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Thank you for this! 
--------------------
http:// 
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Midnight Cyclone
StrangerDanger
Registered: 05/29/13
Posts: 399
Loc: oo ess aye oo ess aye
Last seen: 10 years, 2 months
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Re: Frank's Proper Cloning Tek [Re: newera]
#18535898 - 07/09/13 07:04 PM (10 years, 9 months ago) |
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Quote:
newera said:
i was hoping to use this tek to clone some of my pans provided i get them to grow, but after reading a little more on them it appears people don't bother to with pans, i guess there so small it would be a little more difficult? or is it just unneccesary?
I've been wondering the same thing myself. They're very small as is, but with cloning you're supposed to look for the largest, most robust fruit anyways. I'd imagine you could do it, it might just take a few grows to get a fruit you could actually work with.
IMO, cloning and isolation are much better routes than MS.
EDIT: Oh and of course, thanks Frank! Youdabest
Edited by Midnight Cyclone (07/09/13 07:06 PM)
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vaneazy
Stranger
Registered: 09/20/12
Posts: 332
Last seen: 10 years, 3 months
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just posting to keep in my threads nice write up frank. im starting agar as soon as i get enough money to order some MEA lol
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Re: Frank's Proper Cloning Tek [Re: hostyle]
#18696120 - 08/12/13 03:51 PM (10 years, 8 months ago) |
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Quote:
fastfred said:
Quote:
You can flame sterilize between each cut, or just flame sterilize between each petri dish or fruit. I use 3 section dishes so I flame sterilize every three cuts.
You should really sterilize between every transfer. I'm guilty of doing once per jar with needles, since it's kind of an all or nothing, but it's a bad habit to get into.
Before working with wild specimens I cut a section of stem, stab with a dental pick, dip for about 5-10 sec in 1:10 bleach, then 5-10 sec in peroxide.
Dental picks and scalpels work the best for tools. Both are pretty cheap so I keep a lot on hand. Wrap them in tinfoil and PC before starting.
My myc. professor had a pretty good system that I use. Get a jar, beaker, or any suitable heavy container, fill with IPA, and keep your tools all tip down in it. Remove a tool and flame it to burn off the alcohol, use it, then return it to the tool jar. Each time you grab a new tool. Since they're sitting in IPA you don't have to really flame sterilize them much, just burn off the alcohol.
You can use methanol, ethanol, denatured alcohol, IPA, etc. (not bleach). IPA and alcohols are hard on the tools and will rust them out, so rinse them off and dry them afterwards.
Hope my tips help with your guide Frank. I learned a few tricks myself reading it. I had previously done too much cutting and foolery. The tear method sounds much quicker and easier. I was using way too many tedious cuts to get down to clean tissue. Thanks for writing this up and reminding me of better ways.
-FF
And thank you for your contribution FF! Sorry it took me two months to realize I hadn't replied.
I think the IPA container sounds great. I keep my tools in a tall half-pint jar as it is so that will be easy to fill.
Quote:
hostyle said:
Hey Frank,
Thank you for posting this, very informative.
You said: " at the center of the stem, near the base."
What are the benefits of cutting clone tissue from the base?
That seems to be the location of the most healthy, vigorous mycelium.
For small fruits like pans, I clone these by placing the first few pins that pop up on to agar directly.
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Skinty
TOP SECRET
Registered: 07/04/13
Posts: 1,150
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Quote:
FrankHorrigan said:
Quote:
Notahacker420 said:
Yes, but I try think of it as productive entertainment, as opposed to work, but after a while, it becomes work, then a pain, then an obsessive passion where it's only driving force is you, lol.
Agreed. I love every second of it.
They call it "work," I just let them think of it that way
The only "work" is the stress at the potential of getting caught. Otherwise it's pure joy - and not just for the end result 
Thank you Frank another awesome addition to yr series - many have helped me on this forum so far but none as much as you :embarassed:
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Pestile
Registered: 05/02/13
Posts: 875
Loc: Northern Europe
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Re: Frank's Proper Cloning Tek [Re: Skinty]
#18715278 - 08/16/13 04:01 PM (10 years, 7 months ago) |
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Amazing write-up! I'll definitely try this tek!
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The Corbett Report
Open Source Intelligence News
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1nsane1
Stranger
Registered: 08/20/13
Posts: 7
Last seen: 10 years, 7 months
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Re: Frank's Proper Cloning Tek [Re: Pestile]
#18738852 - 08/21/13 05:44 PM (10 years, 7 months ago) |
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nice tek thanks for sharing. think ill be testing that out.
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gramsp
Magic Bean Buyer
Registered: 02/02/14
Posts: 64
Loc:
Last seen: 4 years, 7 days
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Quote:
FrankHorrigan said:
Some fruits will give you an isolate right off the bat, but a lot of mushrooms are actually composites of multiple genetics. However, I have seen TCs say that the original clone sectors perform better together than as an isolate of each. So it's up to you if you decide to isolate the clone further
This is really interesting. Over and over again, in the growing of all things, I find that polyculture often works better than monoculture.
--------------------
Start here. It is the best $9 you will ever spend, and an absolute must for the first-time grower.
How Frank gets things done
Learned the basics? Read this next (Frank's Tips & Tricks)
RogerRabbit said:
Before you tell us to go to science class(I have two engineering degrees-do you?), you should go back to second grade to learn the difference between a whole and a hole.
RR
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Re: Frank's Proper Cloning Tek [Re: gramsp]
#19698895 - 03/15/14 05:32 AM (10 years, 1 month ago) |
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But you can't save and use a polyculture the way you can a monoculture.
Isolates are used for consistency. And when you do find a good fruiter, watch out! 
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grind1
THAT GUY
Registered: 04/03/14
Posts: 46
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I am new to everything and have been reading a lot but one part I dont understand and it wasnt mentioned is once your done with this tek how do you go about inoculating your grain or moving it to your grain.
Do you just remove mycellium thats showing growing or what? I just want a clear understanding beginning to end. Everything is so concentrated on cleanliness and being through. If you mess up here then its a wrap.
I plan to start a mono tub project as my first grow. From everything I have been reading and researching I feel comfortable and confident with the task. In my opinion the amount of time and effort it takes to do cakes it not much more effort to do a bulk grow
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I HAVE SO MUCH GYPSUM, WBS AND COCO COIR ITS RIDICULOUS!!!
The world moves aside for one who knows where their going!
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qwobbler
The Hunter
Registered: 01/14/15
Posts: 177
Loc: Tropics 
Last seen: 6 years, 3 months
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sorry for being a noob, but just making sure....
when you use this method add put the piece of tissue in the agar, how long does it take to colonise from this point?
and then, do you just cut a piece of myc from the agar and add it to a sterile jar of grain?
Cheers
I failed 3 times with spore prints :-(
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PirateSwazey
Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Frank's Proper Cloning Tek [Re: qwobbler]
#21141008 - 01/18/15 07:35 PM (9 years, 2 months ago) |
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After a few days the tissue will start growing out on the plate. As soon as there is enough growth to grab a tiny piece, about the size of a grain of sushi rice, you want to transfer it another agar plate. Doing a couple transfers is pretty standard procedure as it ensures you will have a clean culture for when you go to inoculate grains.
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cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 2 months
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   It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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newera
Stranger
Registered: 03/05/13
Posts: 359
Last seen: 4 years, 3 months
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Re: Frank's Proper Cloning Tek [Re: cronicr]
#22141805 - 08/25/15 04:48 PM (8 years, 7 months ago) |
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If i can get one fruit this year i'll try this
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bodhisatta  
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Frank's Proper Cloning Tek [Re: newera]
#22141839 - 08/25/15 04:54 PM (8 years, 7 months ago) |
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It's stupidly easy, but learning, practice, and experience are the hard part. Do as many as possible at first to increase your chances of spectacular success to go along with inevitable uh ohs
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newera
Stranger
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Re: Frank's Proper Cloning Tek [Re: bodhisatta]
#22186093 - 09/04/15 12:49 AM (8 years, 7 months ago) |
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i'm thinking i may have to just order some syringes, this whole agar thing just ain't working out for me need to get to a fruit before i can clone
Edited by newera (09/04/15 12:49 AM)
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disassembler
Stranger
Registered: 01/14/16
Posts: 32
Loc: Ukraine 
Last seen: 4 years, 4 months
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Very useful tek, short and clear, bookmarked it. 
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Jahnobi
Stranger
Registered: 12/09/15
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Last seen: 7 years, 4 months
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as always frank, your an enormous help.
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johnathanhayes1969
Stranger
Registered: 11/03/16
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Awsome
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Kenetic
Nam Sayin
Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
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Fanboi
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Todo Cambia
  
 
DMT said: Everyone know's me, they just don't know it yet
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nwcleaningllc
THCmastera
Registered: 06/05/13
Posts: 215
Last seen: 5 years, 2 months
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What do you mean by wrapping the petri dish?
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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You have to wrap it otherwise the two halves come apart and there's nothing keeping contaminants out
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nwcleaningllc
THCmastera
Registered: 06/05/13
Posts: 215
Last seen: 5 years, 2 months
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 Re: Frank's Proper Cloning Tek [Re: bodhisatta]
#24553302 - 08/14/17 02:00 PM (6 years, 7 months ago) |
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Got it. Thank you bod, just watched your videos 
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Haha bet that helped
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thewiseguywithaj
Head Fungus
Registered: 02/08/17
Posts: 60
Last seen: 1 month, 28 days
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I read somewhere that you should wipe down the outside of the mushroom with iso before opening it up to kill any surface contam. Is this recommended?
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Martinsapin
no-mad
Registered: 09/12/16
Posts: 310
Loc: Canada
Last seen: 1 year, 10 months
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No need, you gonna take an intern piece of tissue. Well I dont.
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looking for a sclerotia producer print
Edited by Martinsapin (10/04/17 09:01 PM)
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Quote:
thewiseguywithaj said:
I read somewhere that you should wipe down the outside of the mushroom with iso before opening it up to kill any surface contam. Is this recommended?
IF you have a hard time you can dunk the fruit in sterilised water or some dilute bleach water. But you likely won't need to bother. Cloning cubes is easy as hell
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thewiseguywithaj
Head Fungus
Registered: 02/08/17
Posts: 60
Last seen: 1 month, 28 days
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Re: Frank's Proper Cloning Tek [Re: Martinsapin]
#24688470 - 10/06/17 01:08 PM (6 years, 6 months ago) |
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Quote:
Martinsapin said:
No need, you gonna take an intern piece of tissue. Well I dont.
I don't expect the piece of tissue to work full time with no pay. Might as well hire him.
JK. thanks for the advice.
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 catnip40
xฬ็
Registered: 03/09/12
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Gingerballs
Stranger
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Re: Frank's Proper Cloning Tek [Re: wire5]
#24940980 - 01/25/18 12:14 PM (6 years, 2 months ago) |
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Great Post thanks for the big help
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nconn32986
Higher Power
Registered: 01/21/18
Posts: 119
Loc: Boston, Texas
Last seen: 2 years, 4 months
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Re: Frank's Proper Cloning Tek [Re: Gingerballs]
#24985607 - 02/11/18 12:11 PM (6 years, 2 months ago) |
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where is the best place for petri dish with agar?
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OriginalGinger
Lackadaisical
Registered: 07/01/17
Posts: 279
Loc: Cape Town, South Africa
Last seen: 1 day, 8 hours
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nice, thank you.
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"On a desperate mission to find a dung lover print!"
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woodpusher420
mad scientist
Registered: 02/13/14
Posts: 103
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Erock
ever learning
Registered: 02/16/18
Posts: 51
Last seen: 1 year, 3 months
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Awesome write up! Thanks.
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fungalwisdom
Stranger
Registered: 02/21/19
Posts: 1
Last seen: 5 years, 1 month
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Can you clone a dried specimen? If so what sort of preparation would you need to do? Re-hydrate, sterilize?
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woofwoof
such mushrooms!
Registered: 01/04/19
Posts: 1,127
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Quote:
fungalwisdom said:
Can you clone a dried specimen? If so what sort of preparation would you need to do? Re-hydrate, sterilize?
I wouldn't bother trying to rehydrate a dried specimen
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ironwaveink
Brokenbacksweatycrack
Registered: 02/13/19
Posts: 36
Loc: CA
Last seen: 4 years, 9 months
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Re: Frank's Proper Cloning Tek [Re: woofwoof]
#25897829 - 03/26/19 11:03 AM (5 years, 23 days ago) |
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Quote:
woofwoof said:
Quote:
fungalwisdom said:
Can you clone a dried specimen? If so what sort of preparation would you need to do? Re-hydrate, sterilize?
I wouldn't bother trying to rehydrate a dried specimen
Yeah I tried that and didn't go to well lol but a fun experiment.
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lilwoman
Stranger
Registered: 05/03/19
Posts: 56
Last seen: 2 years, 5 months
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Funky Monkey
Human Suppository
Registered: 05/14/19
Posts: 1,099
Loc: In your MOM's poop shoot
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Re: Frank's Proper Cloning Tek [Re: lilwoman] 1
#26120543 - 07/20/19 08:10 PM (4 years, 8 months ago) |
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Quote:
lilwoman said:
Funny that this thread got bumped today, I was JUST using it.
Edited by Funky Monkey (09/21/20 09:48 PM)
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Ababyphoenix
You
Registered: 03/21/19
Posts: 1,015
Loc: Human Body
Last seen: 1 month, 25 days
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Bookmarked
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     Pho's MushiAventures:
 My '21 GLOG  LAGM 2022
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Abby
Stranger
Registered: 09/21/20
Posts: 10
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Thanks Frank.
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Jason400
Stranger
Registered: 07/08/20
Posts: 6
Last seen: 2 years, 4 days
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So I am new at mushroom cultivation but my background is in the Bacterial, Cell, and Virus production in the vaccine industry.
I just started some plates using PC B+ a few days ago. My plan was to transfer the polyclonal mix to grain spawn then to tubs. From there I would choose the fruits with the best phenotypes and start cloning from there.
Are there any draw backs to this procedure. Any advise is welcome.
Thanks in advance.
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JSE
Stranger
Registered: 09/23/21
Posts: 116
Last seen: 3 hours, 19 minutes
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Beginner here... can I ask please... why/when would you clone, as opposed to taking a spore print and inoculating the agar from that?
Thank you
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JrsyHomegrown
NooB
Registered: 01/16/23
Posts: 33
Loc: Shhh
Last seen: 10 days, 8 hours
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 Re: Frank's Proper Cloning Tek [Re: gizmo1]
#28164445 - 01/30/23 02:03 PM (1 year, 2 months ago) |
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Quote:
gizmo1 said:
I have been taking my tissue samples straight to grain risky I know but hasn't failed me yet(knock on wood).
what's your success rate?
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