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Offlineflybynite
amatuer

Registered: 08/03/03
Posts: 57
Loc: Hyperspace
Last seen: 12 years, 5 months
2 Questions on syringe inoculation
    #1777861 - 08/04/03 12:13 PM (13 years, 4 months ago)

OK - the newbie's back with a few more questions. After reviewing the ups and downs of my first inoculation, I have 2 questions:

1). Should I flame the needle between jars? I flamed it before I started and was very careful to only go in and out of the lid holes and not touch anything else with it. I tried to complete the process quickly and accuratly, although one jar I know got almost 1.5 cc when the plunger stuck and the last two got shortchanged a bit each receiving aboout .5cc

2). Is it normal for the syringe to drip a bit in between jars? I would think that the syringe would form a vacuum, but mine would drip
a little between jars.

Thanks to everyomne for their advice. I await my first signs of myocelial growth!


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Deep into that darkness peering long I stood there wondering fearing, doubting, dreaming dreams no mortal ever dreamed before....


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Offlinedankhead420
I have the keyto escapereality

Registered: 02/02/03
Posts: 114
Loc: going to hell in bucket
Last seen: 13 years, 1 month
Re: 2 Questions on syringe inoculation [Re: flybynite]
    #1777870 - 08/04/03 12:16 PM (13 years, 4 months ago)

I usually just swap with alcohol inbetween jars and yes it is normal to drip


--------------------
trippin with little baby jesus


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Offlinejba6511
addict
Registered: 11/04/02
Posts: 495
Last seen: 8 years, 11 months
Re: 2 Questions on syringe inoculation [Re: flybynite]
    #1777873 - 08/04/03 12:18 PM (13 years, 4 months ago)

my friend does all innoc. in a glove box. he lysols everything and flames the needle BEFORE putting into the box. From here he innoc. in the lysoled box and doesn't reflame in between jars. However, his needle does not drip as you described. No problems yet using this method


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: jba6511]
    #1778002 - 08/04/03 01:25 PM (13 years, 4 months ago)

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Offlinekhemfusion
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Registered: 06/24/03
Posts: 216
Loc: West Coast Canada
Last seen: 11 years, 5 months
Re: 2 Questions on syringe inoculation [Re: LiL_KuSsH]
    #1778018 - 08/04/03 01:31 PM (13 years, 4 months ago)

after you spray lysol inside the glovebox, don't try to flame sterilize the needle again, inside the box, trust me on this one, you get a cool blue fireball inside your glovebox, DON'T ASK ME HOW I KNOW

in other news my friends jars are colonizing quite nicely


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: khemfusion]
    #1778032 - 08/04/03 01:37 PM (13 years, 4 months ago)

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OfflineDemiurge
creator ofworlds

Registered: 04/11/03
Posts: 693
Loc: Florida
Last seen: 5 years, 8 months
Re: 2 Questions on syringe inoculation [Re: LiL_KuSsH]
    #1778305 - 08/04/03 03:19 PM (13 years, 4 months ago)

It would be a good idea to sterilize in between jars, especially when doing 4-hole pf jars. But my foaf usually doesn't flame it to red, like he does on the initial sterilization. Just a little alcohol and a quick swipe of the flame should be enough to kill any new bacteria that has fallen onto the syringe. Drips are normal when using large gauged needles, but it shouldn't drip a lot.


--------------------
Just another animal embeded in a technological coral reef extruded psychic objects...


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OfflineKoOs
BlingBling!
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Registered: 02/13/02
Posts: 6,226
Loc: Glendive MT
Last seen: 3 years, 1 month
Re: 2 Questions on syringe inoculation [Re: flybynite]
    #1778315 - 08/04/03 03:22 PM (13 years, 4 months ago)

just swab with alcohol inbetween jars is all you need to do.


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Offlinenone
newbie
Registered: 08/02/03
Posts: 34
Last seen: 13 years, 3 months
Re: 2 Questions on syringe inoculation [Re: KoOs]
    #1778354 - 08/04/03 03:40 PM (13 years, 4 months ago)

i've read the best way to do it is to flame steralize your needle red hot with an alcohol flame and dunk in alocohol to cool off needle and give a little shake and inoculate.

"give a little shake and inoculate"


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Offlineflybynite
amatuer

Registered: 08/03/03
Posts: 57
Loc: Hyperspace
Last seen: 12 years, 5 months
Re: 2 Questions on syringe inoculation [Re: none]
    #1778458 - 08/04/03 04:22 PM (13 years, 4 months ago)

Many thanks to everyone for the insight. I did not sterilize between jars, but was working in very clean conditions. Washed all surfaces with anti bacterial soap, showered, wore clean clothes, scrubbed hands and arms with anti bac soap, used gloves and wiped them down with alcohol before starting. I'll let you know if I get any significant infection in any jars.


--------------------
Deep into that darkness peering long I stood there wondering fearing, doubting, dreaming dreams no mortal ever dreamed before....


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