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FuckUp Eddy
Mad Scientist



Registered: 10/29/12
Posts: 84
Last seen: 10 years, 11 months
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Proofing Strains
#17764291 - 02/07/13 08:55 PM (11 years, 3 months ago) |
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I just finished a series of isolations and used agar wedge to BRF. Lysol nutra-air works wonders...I haven't had one contam even with agar wedge to BRF (polyfill) no flow hood. Out of eight jars, only four have colonized. The other isolates kind of puttered out 1/5th of the way through the substrate. And those particular isolates are very cottony, almost like white mold. But I know it is not mold because the other four showed the same kind of slow extremely cottony growth, and then took off rizomorphic like crazy. Before this I fruited a monster from a cake that exhibited the same type of weird growth before it took off rizomorphic. My question is, what is that weird growth initially? Keep it mind that some whole jars come out with nothing but that growth. Im tempted to assume that maybe I haven't isolated "enough" and that those are just poor performers that will hardly colonize or fruit? Has anyone else experienced this?
Picture blurry...but you can see cottony growth at the top of the jar with much different texture. Then it really starts tearing through the sub. Some of my jars only get the cotton, and no rizos, and I assume won't fruit to well either.

Before this I had never done agar to BRF...so I tested my skills by throwing a little wedge on BRF, during perhaps my second transfer. By my third transfer it had already colonized the cake. I went on Christmas holiday and left the damn thing to fruit in a cold room, uncared for. When I got back I saw the biggest mushroom I have grown to date! And let me tell you what...it kicked ass. Im tempted to believe that the cold fruiting made a bit of difference. I went back and researched the forum and it seems Im not the only one who agrees with that theory. So now I know those genetics are somewhere in one of the four jars Im about to fruit. Can't wait to get a master going!
-------------------- If you havent played with this then you are missin out! http://www.albinoblacksheep.com/flash/zoomquilt Also good video!!! http://www.youtube.com/watch?v=6O6x_m4zvFs
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FuckUp Eddy
Mad Scientist



Registered: 10/29/12
Posts: 84
Last seen: 10 years, 11 months
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Bump...two hours short but...bump.
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krypto2000
Unknown


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I forget the names, but there's two types of cottony growth. I'm not sure which one yours is, but it's either good or bad. The bad is that thet the two strains can't combine to form.. I don't know the name of that either, but it's what's essential to fruiting, like a sperm and an egg kinda. Or it means it's thriving on your spawn material and is very well suiting for whatever you're using. Sorry I couldn't completely answer this, but someone can hopefully come fill in the gaps and maybe identify which one of those is going on.
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FuckUp Eddy
Mad Scientist



Registered: 10/29/12
Posts: 84
Last seen: 10 years, 11 months
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Like I said though, the cakes with just a small amount of this growth are fruiting very well. Its only when there's nothing but this growth that the cake doesn't fruit. Maybe its just a shitty non performer in there?
-------------------- If you havent played with this then you are missin out! http://www.albinoblacksheep.com/flash/zoomquilt Also good video!!! http://www.youtube.com/watch?v=6O6x_m4zvFs
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ArmFromTheAbyss
Old Hand

Registered: 10/09/02
Posts: 1,368
Loc: Down here in Babylon
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Quote:
FuckUp Eddy said: But I know it is not mold because the other four showed the same kind of slow extremely cottony growth, and then took off rizomorphic like crazy. Before this I fruited a monster from a cake that exhibited the same type of weird growth before it took off rizomorphic.
Your answer is within the question. Morphology of mycelium can change based on differences in conditions of the substrate. Moisture and nutrient content as well as c02 levels are the factors involved. Therefore isolating a rhizomorph on agar does not guarantee you will have rhizos when you transfer to a different substrate. Conversely, it is possible to isolate cottony mycelium on agar that will turn rhizomorphic once transferred to something else. I disagree with the conventional method of agar isolation, because I don't believe morphology is correlated to fruiting performance.
I can't prove this but my experience and reasoning leads me to believe that mycelium tends to be cottony on nutrient rich/moist substrates. Dryer and less nutrient dense substrates tend to produce rhizomorphs. When the organism has no incentive to explore because the substrate is rich it will hesitate to produce rhizomorphs.
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FuckUp Eddy
Mad Scientist



Registered: 10/29/12
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Last seen: 10 years, 11 months
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I kind of understand what you are saying. But the substrate is the same...BRF verm. Cottony on top, rizomorphic the rest of the substrate. I dont see where the substrate comes into play regarding the issue. Im just wondering why its doing what its doing.
-------------------- If you havent played with this then you are missin out! http://www.albinoblacksheep.com/flash/zoomquilt Also good video!!! http://www.youtube.com/watch?v=6O6x_m4zvFs
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
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-------------------- LAGM v 2.024 - endo cabendo
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FuckUp Eddy
Mad Scientist



Registered: 10/29/12
Posts: 84
Last seen: 10 years, 11 months
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Well no need for the forum then since we all have google!
-------------------- If you havent played with this then you are missin out! http://www.albinoblacksheep.com/flash/zoomquilt Also good video!!! http://www.youtube.com/watch?v=6O6x_m4zvFs
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
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Quote:
FuckUp Eddy said: Well no need for the forum then since we all have google!
And since google indexes shroomery, it redirects you back to this forum.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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FuckUp Eddy
Mad Scientist



Registered: 10/29/12
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Last seen: 10 years, 11 months
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Re: Proofing Strains [Re: PussyFart]
#17776889 - 02/10/13 11:10 AM (11 years, 3 months ago) |
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Wheres RR...thats about the only thing that doesn't pop up on google. I bet if we had to pay 25 cents to have a question answered, then everyone would be happier. Hey! Business opportunity!
-------------------- If you havent played with this then you are missin out! http://www.albinoblacksheep.com/flash/zoomquilt Also good video!!! http://www.youtube.com/watch?v=6O6x_m4zvFs
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
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are you dropping agar onto the top of a brf cake? If it were WBS I'd say shake it up, honestly I didn't think agar to brf was..."a thing" 
sorry if the link wasn't any help, thought it may have had some valid info
-------------------- LAGM v 2.024 - endo cabendo
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FuckUp Eddy
Mad Scientist



Registered: 10/29/12
Posts: 84
Last seen: 10 years, 11 months
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Yes, I am dropping an small agar wedge on top of brf, and that is where the cottony growth starts. Sometimes it continues to have nothing but cottony growth, and that cake fruits poorly or not at all. Others start out cottony, and then take off rizomorphic. These fruit very well in the rizomorphic areas of the cake.
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 5 days, 23 hours
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you said you just finished a series of isolations right? did you start from spore on agar or from tissue sample on agar?
if it was from tissue culture, was it from a fruit that came from a later flush? could it just be age and rhizo breaking down?
and you have a poly-fil type filter lid on that jar right?
-------------------- LAGM v 2.024 - endo cabendo
Edited by MonkeyJesusFresco (02/10/13 08:51 PM)
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FuckUp Eddy
Mad Scientist



Registered: 10/29/12
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polyfill...and I started with spores.
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 5 days, 23 hours
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I bet if you just spawn it to bulk it'll flush out alright
-------------------- LAGM v 2.024 - endo cabendo
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
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If you did a series of isolations, then the rest are probably better than this crappy one. Normally, a single swipe of spores on agar will deliver dozens of isolated single-sector strains.
You did inoculate other brf jars with the rest of the isolates didn't you? Trying to find a good strain one at a time could take a lifetime. If you fruit a few dozen isolates simultaneously you'll find the stellar performer much faster. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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FuckUp Eddy
Mad Scientist



Registered: 10/29/12
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Last seen: 10 years, 11 months
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Yes Im fruiting the other ones as well. Some are barely colonizing with cottony mycellium. Others have that same cottony growth but then 1/5th the way down the jar, they take off.
Im assuming also that after my isolations I could have more than one stellar fruiting performer?
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krypto2000
Unknown


Registered: 12/05/06
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I believe I remember RR saying out of 20 isos 2 will be good 2 will be bad and the rest will fall in the middle on average.
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