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OfflineKing Reishi
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Registered: 01/11/13
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petri dish
    #17750817 - 02/05/13 04:51 PM (11 years, 3 months ago)

i just put a couple of drops of liquid culture (reishi)) on a plate.

then para filmed (wraped to hold together but not wraped perfect 1/2 in. parafilm) then placed in clen zip lock bag.

should i take it out of the ziplock bag?


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

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Offlinemycofever
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Re: petri dish [Re: King Reishi]
    #17751040 - 02/05/13 05:36 PM (11 years, 3 months ago)

As long as you have a good seal all the way around then you will be fine. I never put mine in zip-lock bags and they never contam I also use 2 inch parafilm. You stated that it was not wrapped perfect I do not know what that means.


--------------------
Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

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OfflineKing Reishi
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Re: petri dish [Re: mycofever]
    #17751070 - 02/05/13 05:42 PM (11 years, 3 months ago)

i can see the crack with no parafilm on it. but it is sealed shut.

if i shook it it wont open.

of coures i wouldnt shake it though.


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

Edited by King Reishi (02/05/13 05:43 PM)

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Offlinemycofever
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Re: petri dish [Re: King Reishi]
    #17751175 - 02/05/13 05:56 PM (11 years, 3 months ago)

I can imagine that 1/2 inch would be hard to use. That is very thin man I would suggest you investing in some 2 inch or at least 1 inch.Since you can see a crack in it just leave it in the zip-lock bag It should be fine in there.


--------------------
Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

Edited by mycofever (02/05/13 05:58 PM)

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OfflineKing Reishi
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Re: petri dish [Re: mycofever]
    #17751196 - 02/05/13 06:00 PM (11 years, 3 months ago)

for sure thxs man. its my first parafilm buy. and it was like $2 free shipping 1080 in. length.

isnt 2 in. too big. what about 1 in.?


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

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Offlinemycofever
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Re: petri dish [Re: mycofever]
    #17751206 - 02/05/13 06:02 PM (11 years, 3 months ago)

Also you have to cover the entire gap from the bottom plate to the top plate. The para-film needs to lay over both sides and be layed down smooth. any open gap between the plates will allow contams to travel through even if the plates are tight together.


--------------------
Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

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Offlinemycofever
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Re: petri dish [Re: mycofever]
    #17751224 - 02/05/13 06:05 PM (11 years, 3 months ago)

I use 2 inch I start at the top corner of my top plate and it wraps around about 1/2 inch onto the very bottom of the dish.It works very well for me 1 inch will be fine but you will just have to adjust where you make your line at.


--------------------
Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

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OfflineKing Reishi
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Re: petri dish [Re: mycofever]
    #17751316 - 02/05/13 06:18 PM (11 years, 3 months ago)

do ziplocks allow for GE?


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

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Offlinemycofever
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Re: petri dish [Re: King Reishi]
    #17751332 - 02/05/13 06:20 PM (11 years, 3 months ago)

no. but if you did not push all of the air out it will colonize.


--------------------
Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

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OfflinePussyFart
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Re: petri dish [Re: King Reishi]
    #17752235 - 02/05/13 08:55 PM (11 years, 3 months ago)

Quote:

King Reishi said:
do ziplocks allow for GE?



Cultures on petri dishes do not need GE.

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OfflineRogerRabbitM
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Re: petri dish [Re: PussyFart]
    #17752643 - 02/05/13 09:56 PM (11 years, 3 months ago)

Yes they do need gas exchange.  This is why we use parafilm.  Petri dishes don't seal just because the lid is on.  They have tabs to keep the lid slightly above the rim.  They must be wrapped all the way around with parafilm.

I put the dishes in ziplock bags after wrapping for an extra layer of protection.  Most ziplock bags also provide gas exchange.  You guys should know that.  Haven't you ever had your pot dry out in a ziplock?
RR


--------------------
Download Let's Grow Mushrooms



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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflinePussyFart
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Re: petri dish [Re: RogerRabbit]
    #17752683 - 02/05/13 10:02 PM (11 years, 3 months ago)

Quote:

RogerRabbit said:
Yes they do need gas exchange.  This is why we use parafilm.  Petri dishes don't seal just because the lid is on.  They have tabs to keep the lid slightly above the rim.  They must be wrapped all the way around with parafilm.
RR



Yes, I know about the tabs, or vents that they put on the dishes, and I know you were about to say this is why we have TC tags, but some of the TCs here tell noobs that dishes do not require gas exchange...

I seriously think you guys need to have a meeting on what is good advice and what is not, because there are just too many different opinions on certain subsjects from TCs that get discussed everyday, and most of the TCs here are not on the same page.

For example, I have seen TL tell noobs countless times that agar dishes do not need GE, but he has a TC tag just like you, so who should I believe?

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OfflineKing Reishi
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Registered: 01/11/13
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Re: petri dish [Re: PussyFart]
    #17752922 - 02/05/13 10:39 PM (11 years, 3 months ago)

Belive RR hes a G.

if he offered me a job id move up there North West in a heart beat lol.


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 days, 20 hours
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Re: petri dish [Re: King Reishi]
    #17752930 - 02/05/13 10:40 PM (11 years, 3 months ago)

Quote:

King Reishi said:
Belive RR hes a G.

if he offered me a job id move up there North West in a heart beat lol.



Haha, me 2 no doubt, but that is not the point here, lol.

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Offlinemycofever
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Re: petri dish [Re: PussyFart]
    #17754201 - 02/06/13 05:53 AM (11 years, 3 months ago)

Yeah I believe we were discussing a petri dish in a zip-lock bag and if it would be okay. Not worshiping rr.


--------------------
Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

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OfflineKing Reishi
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Re: petri dish [Re: mycofever]
    #17755311 - 02/06/13 12:06 PM (11 years, 3 months ago)

lol not worship apprication.

u know u think RR is a G


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

Extras: Filter Print Post Top
Offlinemycofever
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Registered: 10/13/12
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Re: petri dish [Re: King Reishi]
    #17755881 - 02/06/13 01:41 PM (11 years, 3 months ago)

I will admit I hope to one day come close to his knowledge in cultivation.But this will never be me. :bow2:    I was just messin though man it's ok, You can do what you want to do.


--------------------
Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

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OfflineGod Stamet
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Registered: 07/01/12
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Re: petri dish [Re: PussyFart]
    #17761407 - 02/07/13 11:41 AM (11 years, 3 months ago)

Quote:

Notahacker420 said:
Quote:

RogerRabbit said:
Yes they do need gas exchange.  This is why we use parafilm.  Petri dishes don't seal just because the lid is on.  They have tabs to keep the lid slightly above the rim.  They must be wrapped all the way around with parafilm.
RR



Yes, I know about the tabs, or vents that they put on the dishes, and I know you were about to say this is why we have TC tags, but some of the TCs here tell noobs that dishes do not require gas exchange...

I seriously think you guys need to have a meeting on what is good advice and what is not, because there are just too many different opinions on certain subsjects from TCs that get discussed everyday, and most of the TCs here are not on the same page.

For example, I have seen TL tell noobs countless times that agar dishes do not need GE, but he has a TC tag just like you, so who should I believe?




all TC's aren't created equal lol. just give advice based on your own experience. that's what i do so i don't just regurgitate stuff i've heard like most people on shroomery.


--------------------
I have caught the Mycology bug! Not just here for the ride, i'm here for life.

How I do my bulk substrate

just another mushroom capsule TEK

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OfflinePussyFart
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Re: petri dish [Re: God Stamet]
    #17761440 - 02/07/13 11:49 AM (11 years, 3 months ago)

Quote:

God Stamet said:
all TC's aren't created equal lol. just give advice based on your own experience. that's what i do so i don't just regurgitate stuff i've heard like most people on shroomery.



This is not the point, one of RR's favorite phrases are "That's why we have a TC tag", but that tag means nothing when we cannot trust the advice.

And when there is conflicting info such as petri's needing GE or not, you would think there could only be one answer.

Or how about tub consolidation, RR constantly tells people to let their bulk subs consolidate, while other TC constantly tell noobs not to let their tubs consolidate.

Noobs, myself included, are being pulled in 2 different directions and it is not right, pick a side, and get some facts straight....or don't have BS TC tags that mean nothing.

I am not going to suffocate some dishes to find out, the info should be here already.

Edited by PussyFart (02/07/13 11:49 AM)

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OfflinePussyFart
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Re: petri dish [Re: PussyFart]
    #17761456 - 02/07/13 11:52 AM (11 years, 3 months ago)

All I try to do everyday is help people grow mushrooms the right way, but with all this conflicting info amongst TCs, who should you trust?

Should you test every possible theory yourself to find every answer? If so, why do we have this site? Or "Trusted Cultivator" tags?

Just me venting, sorry, but it is the truth, in my head at least.

Edited by PussyFart (02/07/13 11:54 AM)

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OfflineGod Stamet
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Posts: 774
Last seen: 9 years, 6 months
Re: petri dish [Re: PussyFart]
    #17761483 - 02/07/13 11:59 AM (11 years, 3 months ago)

like i said, information that comes from experience will always be solid. if you don't know from experience you won't for sure be helping anyone.


--------------------
I have caught the Mycology bug! Not just here for the ride, i'm here for life.

How I do my bulk substrate

just another mushroom capsule TEK

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OfflinePussyFart
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Re: petri dish [Re: God Stamet]
    #17761528 - 02/07/13 12:09 PM (11 years, 3 months ago)

Quote:

God Stamet said:
like i said, information that comes from experience will always be solid. if you don't know from experience you won't for sure be helping anyone.



I'm sorry, I thought that was why we have TC tags, so we get advice from people with experience.

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OfflineKing Reishi
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Re: petri dish [Re: PussyFart]
    #17762415 - 02/07/13 02:57 PM (11 years, 3 months ago)

i feel u man. and u do always try to help people. but dont try and take it to the heart. maybe they where just mistaken.

TC's r human as well.


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

Extras: Filter Print Post Top
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