Home | Community | Message Board


Vaposhop
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars

Jump to first unread post. Pages: 1
Offlinejoeinc
journeyman
Male
Registered: 07/01/02
Posts: 113
Loc: Brooklyn, NYC
Last seen: 11 years, 8 months
sterilizing water for syringe making
    #1773939 - 08/02/03 10:30 PM (14 years, 9 months ago)

Wondering if this is an acceptable way to sterilize some water to make sysringes...I'm thinking of taking a small poland spring water bottle.. sealed ... put in in the microwave on a pulse cycle (15 seconds on 15 seconds off for 3-5 minutes and let it cool... i would draw water by piercing the bottle with the syringe instead of opening it... anyone think this would work?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineTaoinShrrom
The Action inInaction

Registered: 07/03/03
Posts: 98
Last seen: 13 years, 5 months
Re: sterilizing water for syringe making [Re: joeinc]
    #1773983 - 08/02/03 10:44 PM (14 years, 9 months ago)

Or um just boil some distilled water and pull it into the syringe and let cool.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 8 years, 5 months
Re: sterilizing water for syringe making [Re: TaoinShrrom]
    #1774387 - 08/03/03 12:58 AM (14 years, 9 months ago)

Yeah that sounds waaaay too complicated for what you need. Boiling tap water makes it pretty damn sterile enough for syringe making.


--------------------
All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineTaoinShrrom
The Action inInaction

Registered: 07/03/03
Posts: 98
Last seen: 13 years, 5 months
Re: sterilizing water for syringe making [Re: kukusz]
    #1774441 - 08/03/03 01:21 AM (14 years, 9 months ago)

Does tap water work allright? I have always been concerned that a high mineral content could cause mycelium to grow?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJohnnyR
mi cixelsyd

Registered: 07/07/02
Posts: 2,187
Loc: a hot place
Last seen: 12 years, 2 months
Re: sterilizing water for syringe making [Re: TaoinShrrom]
    #1774481 - 08/03/03 01:32 AM (14 years, 9 months ago)

u want mycelium...dont u???


--------------------
-----------------------------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineTaoinShrrom
The Action inInaction

Registered: 07/03/03
Posts: 98
Last seen: 13 years, 5 months
Re: sterilizing water for syringe making [Re: JohnnyR]
    #1774497 - 08/03/03 01:36 AM (14 years, 9 months ago)

Hmm, but if I was keeping the syringe stored wouldnt it just die off after a while?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 8 years, 5 months
Re: sterilizing water for syringe making [Re: TaoinShrrom]
    #1774504 - 08/03/03 01:39 AM (14 years, 9 months ago)

Wait what? You are trying to store mycelium in a syringe or spores? Normally syringes are used to store spores for innoculation, and they last a long time (more than a year pretty sure). If you want to store mycelium (such as honey-tek or something) you can keep those in the fridge too. My friend has used four month old liquid mycelium to start popcorn and such, works great.


--------------------
All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineTaoinShrrom
The Action inInaction

Registered: 07/03/03
Posts: 98
Last seen: 13 years, 5 months
Re: sterilizing water for syringe making [Re: kukusz]
    #1774523 - 08/03/03 01:50 AM (14 years, 9 months ago)

No I mean, if my tap water has high mineral content, very hard water. Can't that cause spores in a syringe to latch on and mycelium start to grow? And if I am storing the syringe for a long time wouldnt everything just sort of die? I guess I am asking if distilled water is really a good idea.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJohnnyR
mi cixelsyd

Registered: 07/07/02
Posts: 2,187
Loc: a hot place
Last seen: 12 years, 2 months
Re: sterilizing water for syringe making [Re: TaoinShrrom]
    #1774532 - 08/03/03 01:56 AM (14 years, 9 months ago)

ya dude... i dunno where u heard about the spores latching on or whatever thats crazy... any water works aslong as you steralize it... dude read a faq... u have a lot to learn...


--------------------
-----------------------------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineTaoinShrrom
The Action inInaction

Registered: 07/03/03
Posts: 98
Last seen: 13 years, 5 months
Re: sterilizing water for syringe making [Re: JohnnyR]
    #1774570 - 08/03/03 02:12 AM (14 years, 9 months ago)

Accutally, its far from unheard of it. A quick search through the contamination forum reveals more than one occurance of it. Here is one with a picture and Anno's reply:

http://www.shroomery.org/forums/showflat...rue#Post1713669


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinejoeinc
journeyman
Male
Registered: 07/01/02
Posts: 113
Loc: Brooklyn, NYC
Last seen: 11 years, 8 months
Re: sterilizing water for syringe making [Re: TaoinShrrom]
    #1774689 - 08/03/03 03:15 AM (14 years, 9 months ago)

Well.... heres my dilemna ... i have two new sterile syringes.... I was hoping to sterilize some water that i could use a few hours later to inject into a half pint sporeprint jar... then let the spore solution hydrate overnight... and use the remaining sterile syringe to inject all of the spore solution into pre sterilzed jars the folowing day...Was planning on flame sterilizing before each loading of spores into the syringe and between injection of each jar to minimize the possibilty of cross contamination...basicly my problem is i dont have enough syringes that i can boil a pot of water and pre-load individual syringes with boiling water... there are going to be 60 jars to innoculate simultaneously


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: joeinc]
    #1775752 - 08/03/03 04:44 PM (14 years, 9 months ago)

Post deleted by administrator


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinejoeinc
journeyman
Male
Registered: 07/01/02
Posts: 113
Loc: Brooklyn, NYC
Last seen: 11 years, 8 months
Re: sterilizing water for syringe making [Re: LiL_KuSsH]
    #1776303 - 08/03/03 08:43 PM (14 years, 9 months ago)

got it... thanks again


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Success rate at first time making a sterile syring tekramrepus 2,974 9 09/18/02 10:23 PM
by PSILLYSIMON
* Injecting cakes with water? Fd3000 2,517 15 04/01/09 09:27 AM
by someman
* a few questions sure to make you moist KeepAskingTime 441 2 08/07/01 04:24 PM
by Shiznitz
* Is it ok to inject the centre of jars? Sterile 539 4 05/09/01 08:49 AM
by Anonymous
* Need advice on sterility & trading spores. The_Gnome_King 1,647 14 06/05/01 12:16 AM
by Azure
* I am scared to do a Print/syring! fearforce7 4,977 18 02/24/17 09:11 PM
by mushpunx
* Attention Newbies-Use Foil to make a sporeprint!
( 1 2 3 all )
Catzeye 14,086 46 02/23/02 02:20 PM
by bluhoney
* Making honey syinges with H2O2???PLEASEREAD Datrutankdogg69 842 6 07/26/01 04:43 PM
by L3D

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
1,075 topic views. 22 members, 113 guests and 9 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Avalon Magic Plants
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.003 seconds on 16 queries.