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Anonymous

Contam eating mycelium?
    #1773439 - 08/02/03 06:50 PM (13 years, 4 months ago)

I listened to a Radio show with Paul Stamets over the internet about a week ago. In this interview he speaks of oyster mycelium being used to clean up oil spills. I'll make this short and sweet. Basically I have kept some jars PF (various substrates) that are contamed. Within the jars there are some very nice bright patches of mycelium. If one were to now isolate this via agar, would one have a more contam resistant strain? If so could it be possible to develope a strain that consumes nothing but contams? Don't know if this has been brought up, but I do appreciate insight as to this experiment is worth carrying out. Thank you for your time :wink: 


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Anonymous

Re: Contam eating mycelium? [Re: ]
    #1774115 - 08/02/03 11:32 PM (13 years, 4 months ago)

Anno, I am counting on you :smile: 


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OfflineMrSleep
P. Cubensis
Registered: 04/05/03
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Re: Contam eating mycelium? [Re: ]
    #1774295 - 08/03/03 12:28 AM (13 years, 4 months ago)

Interesting idea.
-Mr.Sleep


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Invisible@cro
new name
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Registered: 12/07/02
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Re: Contam eating mycelium? [Re: ]
    #1774921 - 08/03/03 06:23 AM (13 years, 4 months ago)

My guess would be this patch really has no special advantage over other genetic variations, it just happened to colonize early and grew till it reached the border with the contam and now it is stalling.  There are certain strains which tend to be more resistant, so your idea is definately a good one but unless the strain is actually overtaking the contam and flourishing in there I think it is probably just coincidence that they germinated close to the same time and grew till they ran into each other.
But then again I'm drunk.  :grin:


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Offlinecerealrat
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Re: Contam eating mycelium? *DELETED* [Re: ]
    #1775827 - 08/03/03 05:14 PM (13 years, 4 months ago)

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Invisiblephobey
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Registered: 03/21/03
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Re: Contam eating mycelium? [Re: cerealrat]
    #1777516 - 08/04/03 07:44 AM (13 years, 4 months ago)

I was thinking about the same thing. Got me a jar where a contam is living from about day 5. But mycelium was growing and growing so i thought i would wait it out. This pic was taken 2 months after innoculation and as u can see the white is very hungry and overlays the green parts with a very thick layer.



Now 3 months further (thas a long time) the jar is fully taken over by the myc. Only some green that show through. Sure as hell im not gone case this but maybe i?ll take it outside and spawn it there. Any ideas on what to with this are welcome.


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OfflineMrSleep
P. Cubensis
Registered: 04/05/03
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Re: Contam eating mycelium? [Re: phobey]
    #1777819 - 08/04/03 11:42 AM (13 years, 4 months ago)

Spawn an outdoor bucket of shit!
-Mr.Sleep


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Invisible2Experimental
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Re: Contam eating mycelium? [Re: MrSleep]
    #1778455 - 08/04/03 04:20 PM (13 years, 4 months ago)

And then send spores or fruits to the pros to clone and see if its contam resistant... or if your a pro, do it yourself


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OfflineRaadt
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Re: Contam eating mycelium? [Re: 2Experimental]
    #1778473 - 08/04/03 04:28 PM (13 years, 4 months ago)

Sending fruits in the mail doesn't seem wise to me. Not to mention it would be difficult to clone.


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Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Invisiblemicro
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Re: mycelium eating Contam? [Re: Raadt]
    #1778903 - 08/04/03 06:39 PM (13 years, 4 months ago)

That's a good question. There are a lot of different species of Trichoderma, alone, and a lot of them could grow off of a nutritious substrtate. T. harzianum is the most common species of mushroom crop contaminant in North America. If you had many jars and only one beat the contaminant I'd say you might have something, however.

--
Micro


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InvisibleLiL_KuSsH
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Deleted [Re: micro]
    #1779138 - 08/04/03 08:05 PM (13 years, 4 months ago)

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Anonymous

Re: mycelium eating Contam? [Re: LiL_KuSsH]
    #1779389 - 08/04/03 09:38 PM (13 years, 4 months ago)

You would isolate the Myc. on agar. As vendors do when they peddle a new strain.


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Anonymous

Re: mycelium eating Contam? [Re: ]
    #1779409 - 08/04/03 09:45 PM (13 years, 4 months ago)

This jar is going on 3 months and is still waging war. Pretty excellent Myc. my dawg thinks:



Hell if they eat oil a lil trich couldn't hurt. May take some work. So the Dawg shall continue with this experiment.......


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InvisibleJared
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Re: mycelium eating Contam? [Re: ]
    #1779498 - 08/04/03 10:09 PM (13 years, 4 months ago)

I've found that even if the mycelium overtakes the trich, even to the point where the jar looks perfect and healthy, the contam seemingly gone.. its just because the mycelium can survive better in the low o2/high co2 envrionment of the jar.. as soon as the cakes are birthed, I've found the contam wakes up and rips through the mycelium.

This probably isn't right.. but its what I've observed.


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Anonymous

Re: mycelium eating Contam? [Re: Jared]
    #1779504 - 08/04/03 10:11 PM (13 years, 4 months ago)

Time will tell, but I do believe the "super strains" were formed in this manner :wink: 


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InvisibleLiL_KuSsH
Lost In The Trip

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Deleted [Re: ]
    #1779518 - 08/04/03 10:14 PM (13 years, 4 months ago)

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Invisiblemicro
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Re: mycelium eating Contam? [Re: LiL_KuSsH]
    #1779679 - 08/04/03 11:28 PM (13 years, 4 months ago)

Quote:

Hell if they eat oil a lil trich couldn't hurt. May take some work.




First of all, I have never heard of cubensis eating oil -- are you talking about oyster mushrooms? Second of all, the bad strain of Trichoderma is a mycoparasite -- it eats the fungal cells by secreting enzymes like chitinases, glucanases and proteases and coiling around the hyphae after accumulating a certain mass of mycelium. It's a lot different than just a competitive strain. You'd have to determine whether the strain is mycoparisitic and not competitive. Mycelium produces natural antibiotics so this really isn't something unusal. There are substances out there that would inhibit Trichoderma, but evolution takes place over a long period of time.

T. harzianum causes $30,000,000 in damages to Agraricus crops a year. People use it as a biocontrol agent for pfytopathogenic fungi; it's a lot different than an agreessive competitive strain of Trichoderms sp. or another green mold.

--
Micro

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Edited by micro (08/04/03 11:29 PM)


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Anonymous

Re: mycelium eating Contam? [Re: micro]
    #1779765 - 08/04/03 11:51 PM (13 years, 4 months ago)

"First of all, I have never heard of cubensis eating oil -- are you talking about oyster mushrooms?"

Didn't you read the first post? It blantently says "OYSTER" Check the similarities of Oyster and Cube Myc.

"Second of all, the bad strain of Trichoderma is a mycoparasite -- it eats the fungal cells by secreting enzymes like chitinases, glucanases and proteases and coiling around the hyphae after accumulating a certain mass of mycelium. It's a lot different than just a competitive strain. You'd have to determine whether the strain is mycoparisitic and not competitive. Mycelium produces natural antibiotics so this really isn't something unusal. There are substances out there that would inhibit Trichoderma, but evolution takes place over a long period of time. "

What makes you think I dont know what the (trich) strain is? Want me to advertise all my dawg's research? He would probably growl at me. I don't like upset dawgs. That's kinda like handing out money? Can I have your PIN number?



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Invisiblemicro
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Re: mycelium eating Contam? [Re: ]
    #1779785 - 08/04/03 11:57 PM (13 years, 4 months ago)

"Didn't you read the first post? It blantently says "OYSTER" Check the similarities of Oyster and Cube Myc."

From where do you come up with that?

"What makes you think I dont know what the (trich) strain is? Want me to advertise all my dawg's research? He would probably growl at me. I don't like upset dawgs. That's kinda like handing out money? Can I have your PIN number?"

Researchers have problems with this sometimes. I work in molecular biology -- what method do you use to determine the strain? I can cite my information. Can you?

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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OfflineMrSleep
P. Cubensis
Registered: 04/05/03
Posts: 804
Last seen: 12 years, 1 month
Re: mycelium eating Contam? [Re: micro]
    #1780060 - 08/05/03 01:25 AM (13 years, 4 months ago)

Ohhhh fuck, schooled by micro. 5 Shrooms for your whack ass micro. My uncle worked in molecular biology, he just died recently due to cancer though...all my relatives think it's funny, I guess it is, lol.
-Mr.Sleep


Edited by MrSleep (08/05/03 01:26 AM)


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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