Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Myyco.com Shop: Isolated Cubensis Liquid Culture For Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineKing Reishi
Ninja
Male


Registered: 01/11/13
Posts: 396
Loc: Nibiru
Last seen: 11 years, 1 month
save agar plate
    #17729817 - 02/01/13 11:19 PM (11 years, 3 months ago)

say i wanna save an agar isolate (para filmed/in ziplock bag)

do i put it in the fridge or freezer?


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 days, 20 hours
Trusted Cultivator
Re: save agar plate [Re: King Reishi]
    #17729861 - 02/01/13 11:29 PM (11 years, 3 months ago)

I leave mine at room temperature until I use them.

Extras: Filter Print Post Top
OfflineKing Reishi
Ninja
Male


Registered: 01/11/13
Posts: 396
Loc: Nibiru
Last seen: 11 years, 1 month
Re: save agar plate [Re: PussyFart]
    #17729892 - 02/01/13 11:34 PM (11 years, 3 months ago)

no i mean i have an isolate and i wanna save it.

do i really have to buy the test tube things.


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

Extras: Filter Print Post Top
InvisibleBeefy1
GONE


Registered: 05/01/09
Posts: 3,573
Loc: around
Re: save agar plate [Re: King Reishi]
    #17729904 - 02/01/13 11:36 PM (11 years, 3 months ago)

Slant?



what species?

Extras: Filter Print Post Top
OfflineKing Reishi
Ninja
Male


Registered: 01/11/13
Posts: 396
Loc: Nibiru
Last seen: 11 years, 1 month
Re: save agar plate [Re: Beefy1]
    #17730173 - 02/02/13 12:27 AM (11 years, 3 months ago)

ya my bad do i really need to get a slant?

Reishi.


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

Extras: Filter Print Post Top
OfflineTerry M
Stranger in a Strange Land
Male


Registered: 06/18/10
Posts: 1,502
Loc: Rhode Island
Last seen: 9 years, 3 months
Re: save agar plate [Re: King Reishi]
    #17731255 - 02/02/13 07:24 AM (11 years, 3 months ago)

You can save it on your petri dish in the refrigerator. Freezing will likely break the cells and kill it. You will have to re-transfer it to a fresh agar dish periodically, however. The same is true for slants, but with slants you have to do it less often.

My own rule of thumb (other people have different ones) is to keep a petri culture no more than 6 months before transferring it or tossing it. For slants, I transfer after 18 months by regrowing it onto an agar dish and reinoculating fresh slant tubes.


--------------------
Liberté, égalité, humidité.

Extras: Filter Print Post Top
Invisibleb plus

Registered: 10/13/12
Posts: 928
Re: save agar plate [Re: Terry M]
    #17731772 - 02/02/13 10:28 AM (11 years, 3 months ago)

slant:thumbup:

play with that bad boy for years


--------------------
:mushroom2:-Reishi Grow-:mushroom2:
:mushroom2:-Grey Oyster Grow-:mushroom2:

Extras: Filter Print Post Top
OfflineKing Reishi
Ninja
Male


Registered: 01/11/13
Posts: 396
Loc: Nibiru
Last seen: 11 years, 1 month
Re: save agar plate [Re: b plus]
    #17732507 - 02/02/13 01:01 PM (11 years, 3 months ago)

why does a slant last longer isnt it pretty much the same thing?


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 days, 20 hours
Trusted Cultivator
Re: save agar plate [Re: King Reishi]
    #17732527 - 02/02/13 01:04 PM (11 years, 3 months ago)

Quote:

King Reishi said:
why does a slant last longer isnt it pretty much the same thing?



A slant will have a higher moisture content, so it will keep the culture longer.

Extras: Filter Print Post Top
OfflineKing Reishi
Ninja
Male


Registered: 01/11/13
Posts: 396
Loc: Nibiru
Last seen: 11 years, 1 month
Re: save agar plate [Re: PussyFart]
    #17732541 - 02/02/13 01:06 PM (11 years, 3 months ago)

aww....

u gotta put those motherfuckers on an angle when they turn into a jello though huh.

do u know a good place i could buy just 1 for cheap?


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 days, 20 hours
Trusted Cultivator
Re: save agar plate [Re: King Reishi]
    #17732573 - 02/02/13 01:12 PM (11 years, 3 months ago)

Our Sponsors.... or Ebay :shrug:

Extras: Filter Print Post Top
OfflineKing Reishi
Ninja
Male


Registered: 01/11/13
Posts: 396
Loc: Nibiru
Last seen: 11 years, 1 month
Re: save agar plate [Re: PussyFart]
    #17732582 - 02/02/13 01:12 PM (11 years, 3 months ago)

fo sho thxs man.

hows ur gows going?


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

Edited by King Reishi (02/02/13 01:13 PM)

Extras: Filter Print Post Top
Offlinemycofever
Part-Time
 User Gallery


Registered: 10/13/12
Posts: 605
Loc: USA Flag
Last seen: 5 years, 1 month
Re: save agar plate [Re: Terry M]
    #17751363 - 02/05/13 06:26 PM (11 years, 3 months ago)

Quote:

Terry M said:
You can save it on your petri dish in the refrigerator. Freezing will likely break the cells and kill it. You will have to re-transfer it to a fresh agar dish periodically, however. The same is true for slants, but with slants you have to do it less often.

My own rule of thumb (other people have different ones) is to keep a petri culture no more than 6 months before transferring it or tossing it. For slants, I transfer after 18 months by regrowing it onto an agar dish and reinoculating fresh slant tubes.




Thant you terry I have been wondering this very thing and you have answered my question without me asking :thumbup:


--------------------
Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

Extras: Filter Print Post Top
Jump to top Pages: 1

Myyco.com Shop: Isolated Cubensis Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* Agar plate info? Joshua 3,273 6 11/02/01 04:36 PM
by dimitri211
* Agar Culture transfer to Liquid Culture Tek Compiler 25,564 11 06/27/03 05:11 PM
by Compiler
* Effect of gravity on agar plate? Mycolomaniac 1,474 3 04/07/22 07:32 PM
by primordialkraken
* agar plate to liquid culture, how do u do it?? Visigoth 7,154 12 07/31/02 03:33 AM
by Suntzu
* Best pre-made agar plates for Cultivation no-tone 3,725 9 01/30/03 10:31 AM
by no-tone
* Alternatives to Agar Plates?
( 1 2 all )
GGreatOne234 5,468 20 09/11/02 07:14 AM
by GGreatOne234
* Culture storage and agar plate alternative. SalviaEngland 1,843 2 01/18/03 05:48 PM
by ISH
* 1st and 2nd Flush from the Agar Plate! jelsert 962 1 12/07/02 01:28 PM
by Skikid16

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
760 topic views. 0 members, 2 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.006 seconds on 14 queries.