|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
King Reishi
Ninja



Registered: 01/11/13
Posts: 396
Loc: Nibiru
Last seen: 11 years, 1 month
|
save agar plate
#17729817 - 02/01/13 11:19 PM (11 years, 3 months ago) |
|
|
say i wanna save an agar isolate (para filmed/in ziplock bag)
do i put it in the fridge or freezer?
|
PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 days, 20 hours
|
|
I leave mine at room temperature until I use them.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
|
King Reishi
Ninja



Registered: 01/11/13
Posts: 396
Loc: Nibiru
Last seen: 11 years, 1 month
|
Re: save agar plate [Re: PussyFart]
#17729892 - 02/01/13 11:34 PM (11 years, 3 months ago) |
|
|
no i mean i have an isolate and i wanna save it.
do i really have to buy the test tube things.
|
Beefy1
GONE


Registered: 05/01/09
Posts: 3,573
Loc: around
|
|
Slant?
what species?
|
King Reishi
Ninja



Registered: 01/11/13
Posts: 396
Loc: Nibiru
Last seen: 11 years, 1 month
|
Re: save agar plate [Re: Beefy1]
#17730173 - 02/02/13 12:27 AM (11 years, 3 months ago) |
|
|
ya my bad do i really need to get a slant?
Reishi.
|
Terry M
Stranger in a Strange Land



Registered: 06/18/10
Posts: 1,502
Loc: Rhode Island
Last seen: 9 years, 3 months
|
|
You can save it on your petri dish in the refrigerator. Freezing will likely break the cells and kill it. You will have to re-transfer it to a fresh agar dish periodically, however. The same is true for slants, but with slants you have to do it less often.
My own rule of thumb (other people have different ones) is to keep a petri culture no more than 6 months before transferring it or tossing it. For slants, I transfer after 18 months by regrowing it onto an agar dish and reinoculating fresh slant tubes.
-------------------- Liberté, égalité, humidité.
|
b plus

Registered: 10/13/12
Posts: 928
|
Re: save agar plate [Re: Terry M]
#17731772 - 02/02/13 10:28 AM (11 years, 3 months ago) |
|
|
slant
play with that bad boy for years
|
King Reishi
Ninja



Registered: 01/11/13
Posts: 396
Loc: Nibiru
Last seen: 11 years, 1 month
|
Re: save agar plate [Re: b plus]
#17732507 - 02/02/13 01:01 PM (11 years, 3 months ago) |
|
|
why does a slant last longer isnt it pretty much the same thing?
|
PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 days, 20 hours
|
|
Quote:
King Reishi said: why does a slant last longer isnt it pretty much the same thing?
A slant will have a higher moisture content, so it will keep the culture longer.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
|
King Reishi
Ninja



Registered: 01/11/13
Posts: 396
Loc: Nibiru
Last seen: 11 years, 1 month
|
Re: save agar plate [Re: PussyFart]
#17732541 - 02/02/13 01:06 PM (11 years, 3 months ago) |
|
|
aww....
u gotta put those motherfuckers on an angle when they turn into a jello though huh.
do u know a good place i could buy just 1 for cheap?
|
PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 days, 20 hours
|
|
Our Sponsors.... or Ebay
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
|
King Reishi
Ninja



Registered: 01/11/13
Posts: 396
Loc: Nibiru
Last seen: 11 years, 1 month
|
Re: save agar plate [Re: PussyFart]
#17732582 - 02/02/13 01:12 PM (11 years, 3 months ago) |
|
|
fo sho thxs man.
hows ur gows going?
Edited by King Reishi (02/02/13 01:13 PM)
|
mycofever
Part-Time



Registered: 10/13/12
Posts: 605
Loc: USA
Last seen: 5 years, 1 month
|
Re: save agar plate [Re: Terry M]
#17751363 - 02/05/13 06:26 PM (11 years, 3 months ago) |
|
|
Quote:
Terry M said: You can save it on your petri dish in the refrigerator. Freezing will likely break the cells and kill it. You will have to re-transfer it to a fresh agar dish periodically, however. The same is true for slants, but with slants you have to do it less often.
My own rule of thumb (other people have different ones) is to keep a petri culture no more than 6 months before transferring it or tossing it. For slants, I transfer after 18 months by regrowing it onto an agar dish and reinoculating fresh slant tubes.
Thant you terry I have been wondering this very thing and you have answered my question without me asking
-------------------- Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.
|
|