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InvisibleFiend_13
Lord

Registered: 07/13/03
Posts: 424
I got green mold. and other strain dint even start colonizin
    #1762280 - 07/30/03 11:22 AM (14 years, 9 months ago)

I got green mold. and other strain dint even start colonizin. I got my 2 spore syinges from TLG. all of the creeper jars got green mold spots and the eq that i got did not even start colonizing none of them.

Anyone have any suggestions. Please add them


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Offlineshakta
Infidel
Registered: 06/03/03
Posts: 2,633
Last seen: 14 years, 9 days
Re: I got green mold. and other strain dint even start colonizin [Re: Fiend_13]
    #1762301 - 07/30/03 11:34 AM (14 years, 9 months ago)

Sounds like your sterility techniques could use some work to me. Maybe I have just been lucky to date, but I have had no problems with contams at all.


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InvisibleFiend_13
Lord

Registered: 07/13/03
Posts: 424
Re: I got green mold. and other strain dint even start colonizin [Re: shakta]
    #1762303 - 07/30/03 11:36 AM (14 years, 9 months ago)

Can you tell me what your steriliation techniques are?


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Offlineshakta
Infidel
Registered: 06/03/03
Posts: 2,633
Last seen: 14 years, 9 days
Re: I got green mold. and other strain dint even start colonizin [Re: Fiend_13]
    #1762323 - 07/30/03 11:50 AM (14 years, 9 months ago)

Sure. Here is what I do for jars in general.

1. Fill them with substrate and put a foil cover on the top of them.
PC them at 15 PSI for an hour and let them cool down
2. Prepare the work area and myself. This involves taking a shower and putting on clean clothes. I then clean my work table off with a bleach solution, and put everything I need on the table. Shut off any fans or AC. Then I wash my hands good with anti-bacterial, put on a mask and gloves, and get to it.
3. I flame the needle of the syringe until it turns red. I then use a paper towel soaked in alcohol to cool the needle off. I usually resterilize the needle every half dozen jars or so.
4. Remove the foil cover, and place it clean side up on the table.
5. Inject the innoculate, and place the foil back on the jar.

That is pretty much it. My grain jars are easier. I use polyfill lids, with a single seperate innoc hole with a piece of electrical tape on it. When I am ready to knock up the jar, I peel the tape back, squirt it, and put the tape back on. I keep all of my jars in a incubator until they are fully colonized. I feel that the increased colonization time you get when the jars are at the correct temps, helps fight the chance of later blooming contams maybe. That part could be BS though. I am still a newbie, and have had good luck with doing things this way. I have not done a whole lot, so like I said I may just be lucky so far. Good Luck!


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Offlinehyper_dermic
stranger withcandy

Registered: 06/26/02
Posts: 736
Loc: the land of excess
Last seen: 13 years, 8 months
Re: I got green mold. and other strain dint even start colonizin [Re: Fiend_13]
    #1767512 - 07/31/03 11:35 PM (14 years, 9 months ago)

buy a pressurecooker,
build a glovebox.

thats all u need to do...
0% contams :smile:

[hyp] 


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: I got green mold. and other strain dint even start colonizin [Re: Fiend_13]
    #1767775 - 08/01/03 01:03 AM (14 years, 9 months ago)

read this twice all of it .
and then read it again .


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