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OfflineGod Stamet
wannabe Stamets


Registered: 07/01/12
Posts: 774
Last seen: 9 years, 6 months
master culture slant question
    #17587885 - 01/21/13 10:00 PM (11 years, 3 months ago)

how long do you guys let your slants grow for before they are stored in the fridge for later use?


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I have caught the Mycology bug! Not just here for the ride, i'm here for life.

How I do my bulk substrate

just another mushroom capsule TEK

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OfflineTmethylM
Smear in the shale
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Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 7 months
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Re: master culture slant question [Re: God Stamet]
    #17587943 - 01/21/13 10:11 PM (11 years, 3 months ago)

Doesn't really matter imo. I have stored them with .5" of growth and fully colonized.
Roll with whatever you got I say. Swap them out once a year if you're storing long-term.


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OfflineGod Stamet
wannabe Stamets


Registered: 07/01/12
Posts: 774
Last seen: 9 years, 6 months
Re: master culture slant question [Re: Tmethyl]
    #17589460 - 01/22/13 09:54 AM (11 years, 3 months ago)

so it's ok to put them in the fridge immeadiately? anyone else have an opinion on this?


--------------------
I have caught the Mycology bug! Not just here for the ride, i'm here for life.

How I do my bulk substrate

just another mushroom capsule TEK

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OfflineJeff
Addict
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Registered: 10/06/12
Posts: 1,488
Last seen: 6 years, 5 months
Re: master culture slant question [Re: Tmethyl]
    #17589496 - 01/22/13 10:05 AM (11 years, 3 months ago)

Immediately.....no.  You need to, at the very least, let the wedge recover.

Quote:

Tmethyl said:
Doesn't really matter imo. I have stored them with .5" of growth and fully colonized.
Roll with whatever you got I say. Swap them out once a year if you're storing long-term.




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Myco-tek

Edited by Jeff (01/22/13 10:43 AM)

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InvisibleTheShroomJew23
The Chemist
Male

Registered: 11/29/07
Posts: 1,611
Loc: Temple
Re: master culture slant question [Re: Jeff]
    #17619430 - 01/27/13 02:53 PM (11 years, 3 months ago)

I drop them to the bottom of the slant, let them recover and grow about 3mm up the slant and then throw them in the fridge. I have an incubator turned down to 8.3 celsius and change them about every eight months. Some samples however need colder or warmer temps if you start to see cellular damage usually apparent as bluing mycelium. I just have found that my friends like where they are right now.


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