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Offlinesagesuber
Expérimentateur
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Registered: 12/20/12
Posts: 32
Loc: Dominica, Grand Fond Flag
Last seen: 10 years, 4 months
Jerusalem Artichoke Agar
    #17583180 - 01/21/13 02:10 AM (11 years, 3 months ago)

I havent posted much under this username but I have had a bit of experience experimenting for a couple of years. My new username is due to me wanting to keep my identity quiet.

Anyway made some agar today and didnt have potatoes to make PDYA.

I looked out at the garden and noticed I had ample Jerusalem Artichokes. They are great for diabetics and also for people with IBS. It also promotes good bacteria in the gut.
I wondered if would help. Help mycelium select good bacteria in the long run.
Anyway with the unretrievable information on what artichokes contain e.g. carbohydrate and other shit. I thought I go out on a limb and make Jerusalem Artichoke Dextrose Yeast Agar.(JADYA)

Also I will be expanding on sorghum grain which is the most readily availble grain without a husk that can hide bacteria etc.
I live in a very remote area the shops are quite a long drive.

My recipe Ingredients: (similar to PDYA)
250ml of rainwater
250ml of water that was used to boil sorghum in (red in color will help identify mycelium growth)
150g Jerusalem artichoke sliced very thinly
9g agar agar
7g dextrose powder
1g brewers yeast

Water was mixed together and artichokes were boiled for 30mins (or long enough to drink two beers)
PC'd for 30mins and poured at 60oC

Planning to grow oyster and shiitake in jar petri dish. (Because I live remotely I just use shallow jars as petri. It takes too long to order real petris.)

Let me know if you want pic's. Otherwise I like to be slack.


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OfflineLennybernadino
Amazon grower
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Registered: 09/16/09
Posts: 772
Loc: Iquitos, Peru
Last seen: 27 days, 3 hours
Re: Jerusalem Artichoke Agar [Re: sagesuber]
    #17584642 - 01/21/13 12:04 PM (11 years, 3 months ago)

Um popcorn eating smiley face

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Offlinepsylosymonreturns
aka Gym Sporrison
Male


Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
Trusted Cultivator
Re: Jerusalem Artichoke Agar [Re: Lennybernadino]
    #17586081 - 01/21/13 04:32 PM (11 years, 3 months ago)

Quote:

Lennybernadino said:
Um popcorn eating smiley face




:popcorn:


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OfflineFruitbuddy
Mycophilic Cultivator
Male

Registered: 09/01/10
Posts: 214
Last seen: 7 years, 6 months
Re: Jerusalem Artichoke Agar [Re: psylosymonreturns]
    #17586871 - 01/21/13 07:06 PM (11 years, 3 months ago)

i don't see a reason why this wouldn't work. jerusalem's artichoke surely has nutrients that support mycelial growth, and the abundance of soluble fiber sure helps it. keep us updated.

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Offlinesagesuber
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Registered: 12/20/12
Posts: 32
Loc: Dominica, Grand Fond Flag
Last seen: 10 years, 4 months
Re: Jerusalem Artichoke Agar [Re: Fruitbuddy]
    #17587235 - 01/21/13 08:17 PM (11 years, 3 months ago)

Just put oyster and shiitake cultures on the agar. Will keep you updated.


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Offlinesagesuber
Expérimentateur
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Registered: 12/20/12
Posts: 32
Loc: Dominica, Grand Fond Flag
Last seen: 10 years, 4 months
Re: Jerusalem Artichoke Agar [Re: sagesuber]
    #17594063 - 01/22/13 10:59 PM (11 years, 3 months ago)

Something weird happened to the agar. The surface of the agar turned into a gel and had a white thread on the surface. It also smelt kind of weird.
Never had this problem before. It could have been:
A) A contamination (bacteria I suspect)
B) A chemical reaction to an aerosol called glen 20
C) 2 Year out of date Agar powder; or,
D) Some chemical reaction to the Jerusalem artichoke.
Anyway I have PC the petri's and disposed the agar. So no photos.
My dextrose powder is also 2yrs old but I doubt that dextrose can go off.
Anyway I will try again and play with the recipe. For the moment I'm going to use Milo because I couldn't be bothered going to the shops and need to get cultures going before the mushrooms get too old.
I have changed the location of my glove box to the newly renovated bathroom. (bleached everything up to 2m high)


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InvisibleSatanschild
goodbye
Registered: 01/16/13
Posts: 281
Re: Jerusalem Artichoke Agar [Re: sagesuber]
    #17596970 - 01/23/13 02:36 PM (11 years, 3 months ago)

Quote:

sagesuber said:
Something weird happened to the agar. The surface of the agar turned into a gel and had a white thread on the surface. It also smelt kind of weird.
Never had this problem before. It could have been:
A) A contamination (bacteria I suspect)
B) A chemical reaction to an aerosol called glen 20
C) 2 Year out of date Agar powder; or,
D) Some chemical reaction to the Jerusalem artichoke.
Anyway I have PC the petri's and disposed the agar. So no photos.
My dextrose powder is also 2yrs old but I doubt that dextrose can go off.
Anyway I will try again and play with the recipe. For the moment I'm going to use Milo because I couldn't be bothered going to the shops and need to get cultures going before the mushrooms get too old.
I have changed the location of my glove box to the newly renovated bathroom. (bleached everything up to 2m high)




Jerusalem artichokes don't contain starch, but they contain inulin, which is more like a complex-sugar I guess.
Try boiling them, they will completely dissolve to a syrupy-liquid.

It also discolors when exposed to oxygen very quickly, to a first brownish then greyish color. And are very smelly...

The white thread might be a contam though.

Edited by Satanschild (01/23/13 03:12 PM)

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