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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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Offlinemaddcowdung3420
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Need Help Please
    #1756372 - 07/28/03 03:49 PM (13 years, 10 months ago)

Is There Any Way To Create More Spore Syringes With The Mycelium I have Growing Now? Say Put Into A Blender ? HOw Would I go About Doing So?


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Invisiblemicro
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Re: Need Help Please [Re: maddcowdung3420]
    #1756380 - 07/28/03 03:51 PM (13 years, 10 months ago)

bags or jars?

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Offlinemaddcowdung3420
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Re: Need Help Please [Re: micro]
    #1756392 - 07/28/03 03:53 PM (13 years, 10 months ago)

they are jars


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Invisiblemicro
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Re: Need Help Please [Re: maddcowdung3420]
    #1756406 - 07/28/03 03:57 PM (13 years, 10 months ago)

It's harder with jars, but you could always do a liquid culture in a glovebox with an inside piece of the cake (sterile.)  With bags you can just shoot some sterile water into them, mix it with the mycelium and draw it back up.  I've heard of people doing this with jars by tilting them and scratching the side but it would require some skill :wink:

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Micro


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(Avatar is Maxxy, a character by Mizzyam, RIP)


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Offlinemaddcowdung3420
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Registered: 07/01/03
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Re: Need Help Please [Re: micro]
    #1756411 - 07/28/03 04:00 PM (13 years, 10 months ago)

thanx for the reply . but how do I go about making a liquid culture?


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OfflineTaoinShrrom
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Registered: 07/03/03
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Re: Need Help Please [Re: maddcowdung3420]
    #1756422 - 07/28/03 04:03 PM (13 years, 10 months ago)

Read the teks under mycelium cultivation. You can try agar, but I wouldn't recommend that with out some expirence. Or try a honey water tek, but instead of innoculating spores, place a piece of colonized cake. Wait for the mycelium to spread through out the honey water, and then shake it and suck it up in a sterile syringe and innoculate with that.


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Invisiblemicro
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Re: Need Help Please [Re: maddcowdung3420]
    #1756423 - 07/28/03 04:03 PM (13 years, 10 months ago)



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(Avatar is Maxxy, a character by Mizzyam, RIP)


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OfflinePsilocybin_monkey
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Re: Need Help Please [Re: micro]
    #1756443 - 07/28/03 04:09 PM (13 years, 10 months ago)

Captain Max's Wino Tek for Syringe Making

The easy way to make sterile spore syringes.

When making syringes, sterility is the key. Everything must be CLEAN! I do it in the kitchen. First I wash and put away all dishes, wipe the counters, sweep the floor, throw out the garbage and then scrub up for the procedure.

Turn on the stove exhaust fan for a while to help suck up dust and particles from the air.

You will need a presure cooker for this. Used pressure cookers can easily be found at thrift stores and Sally Annes for about $5.

Equipment needed:

Regular wine glass
Cover plate
Scapel or sharp, non-serated knife
Sterile syringes
Spore print

Put wine glass in pressure cooker, lay knife on top. Pressure cook for about 10 - 15 minutes to sterilize.

Have clean tissue ready. Wrap tip of knife in clean tissue. Remove glass and place cover plate over the top.

Boil some water. Take sterile syringe and suck up and squirt out boiling wate a couple times. If making 5 syringes, squirt 5 syringe fulls of boiling water into wine glass. Immediately replace the cover plate. Cool in freezer or if you have time, just let it sit for a couple hours. It must be cold before next step. Not warm, COLD. Or at least, room temp.

Remove tissue from knife and cover plate from glass. Working surfaces are now exposed to the air, work quickly.

Scrape spores into glass. Don't let the knife tip touch anything other than the spore print. Replace cover plate.

Sometimes I like to let the spores sit for a while so they can rehydrate and break up a bit. Now comes the tricky part.

At this point, your syringes should be out of the packages and lined up ready to go. Remove tip of syringe and hold syringe in one hand. Don't let the tip touch anything. With other hand, remove the cover plate and slowly swirl wine glass. With a bit of practice you can soon have the water spinning in the glass like water down a flushed toilet! The spores will dissipate. Suck up first syringe full while water is still spinning and spores are distributed throughout. Keep spinning the water and sucking up your syringes until finished.

Final step: Fill wine glass with wine and toast your upcoming mushroom harvest!

Good luck!

Captain Max Mushroom


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