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OfflineFuckUp Eddy
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Registered: 10/29/12
Posts: 84
Last seen: 10 years, 11 months
"sub-strain"
    #17557914 - 01/16/13 04:36 PM (11 years, 4 months ago)

I need some clarification on a couple things. I looked the information up and was able to obtain bits and pieces (through reading people argue) that I need to put together.

Is the word sub-strain even a word? Wouldn't it be more like a "child" of that particular strain...which exhibits certain genetic traits due to monokaryotic(single nucleus)pairing?

When I isolate mycellium, am I isolating hyphae that have already combined? And is the mycellium itself nothing more than thousands of "ropes" of combined hyphae? If this is so...then what matter is the actual fruit body made up of, and where does it get its traits from? One "dikaryon" in that tangled mess of paired hyphae?


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If you havent played with this then you are missin out! http://www.albinoblacksheep.com/flash/zoomquilt

Also good video!!! http://www.youtube.com/watch?v=6O6x_m4zvFs

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InvisibleArmFromTheAbyss
Old Hand

Registered: 10/09/02
Posts: 1,368
Loc: Down here in Babylon
Re: "sub-strain" [Re: FuckUp Eddy]
    #17561371 - 01/17/13 08:47 AM (11 years, 4 months ago)

Quote:

FuckUp Eddy said:
When I isolate mycellium, am I isolating hyphae that have already combined?




Yes. Unless you're using a microscope to isolate hyphae. When a spore germinates it forms a strand of hyphae. When two strands of hyphae join together it's called mycelium. When you do your first isolation and extract a tiny bit of mycelium there will be many "strains" all jumbled together. By transferring a small piece of mycelium you're reducing the overall amount of strains compared to the original MS culture. As you do this a few times you'll narrow it down to just one strain.

A strain is formed whenever two spores germinate and join into mycelium. A drop of MS solution could produce countless different genetic strains.


Quote:

FuckUp Eddy said:
And is the mycellium itself nothing more than thousands of "ropes" of combined hyphae? If this is so...then what matter is the actual fruit body made up of, and where does it get its traits from? One "dikaryon" in that tangled mess of paired hyphae?




A fruiting body is essentially mycelium that has formed into a mass. Fungi are totipotent, meaning you can form a fruiting body from mycelium or you can form mycelium from a fruiting body.

http://bugs.bio.usyd.edu.au/learning/resources/Mycology/Growth_Dev/hyphalGrowth.shtml


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OfflineFuckUp Eddy
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Re: "sub-strain" [Re: ArmFromTheAbyss]
    #17565309 - 01/17/13 11:06 PM (11 years, 3 months ago)

SO by fruiting an isolate, the resulting fruit body will (should) be the phenotype of one strand/strain (infinitely branched of course) of mycellium (combined hyphae).

Also the fruit body itself doesn't really resemble ropey web like mycellium. How can it go from being one texture to another? What is happening?

And with a multispore grow...is one strain (strand of mycellium) going to determine the phenotype of a certain fruit, or do other strains have a factor in the phenotype of that fruit. See what Im sayin?


--------------------
If you havent played with this then you are missin out! http://www.albinoblacksheep.com/flash/zoomquilt

Also good video!!! http://www.youtube.com/watch?v=6O6x_m4zvFs

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Offlinethiotimoline
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Re: "sub-strain" [Re: FuckUp Eddy]
    #17566905 - 01/18/13 09:17 AM (11 years, 3 months ago)

Clones from a single fruit often result in sectoring so many genetic strains must be present.

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OfflineRogerRabbitM
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Re: "sub-strain" [Re: thiotimoline]
    #17567145 - 01/18/13 10:25 AM (11 years, 3 months ago)

Quote:

Is the word sub-strain even a word? Wouldn't it be more like a "child" of that particular strain...which exhibits certain genetic traits due to monokaryotic(single nucleus)pairing?




Sort of.

There often will be many millions of germinated spores and therefore many thousands of dikaryotic pairings when a sporeprint is used.  Each of these pairings is by definition a strain.  Many of these strains will be compatible and will experience dikaryotic/dikaryotic pairings, while others will remain separate.  The mycologists' job is to fruit out each one to find the stellar performer(s).
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineFuckUp Eddy
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Re: "sub-strain" [Re: RogerRabbit]
    #17570907 - 01/18/13 11:30 PM (11 years, 3 months ago)

And the phenotype/genetics of the fruit come from? And the composition of the fruit body is made up of what exactly? It doesn't seem to resemble mycellium...more like some soft rubber.


--------------------
If you havent played with this then you are missin out! http://www.albinoblacksheep.com/flash/zoomquilt

Also good video!!! http://www.youtube.com/watch?v=6O6x_m4zvFs

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Offlinepseudotsuga
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Registered: 06/29/11
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Re: "sub-strain" [Re: FuckUp Eddy]
    #17571957 - 01/19/13 08:08 AM (11 years, 3 months ago)

Quote:

FuckUp Eddy said:
And the composition of the fruit body is made up of what exactly? It doesn't seem to resemble mycellium...more like some soft rubber.



It is tightly bound mycelium, nothing more.

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OfflineRogerRabbitM
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Re: "sub-strain" [Re: FuckUp Eddy]
    #17571988 - 01/19/13 08:21 AM (11 years, 3 months ago)

Quote:

FuckUp Eddy said:
And the phenotype/genetics of the fruit come from? And the composition of the fruit body is made up of what exactly? It doesn't seem to resemble mycellium...more like some soft rubber.




This indicates bacteria contamination.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineFuckUp Eddy
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Registered: 10/29/12
Posts: 84
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Re: "sub-strain" [Re: RogerRabbit]
    #17575034 - 01/19/13 06:57 PM (11 years, 3 months ago)

Im referring to the over all texture of the fruit body. Is it straight mycellium, and why does it appear different in general... different then colonizing mycellium. Is it swollen or something?


--------------------
If you havent played with this then you are missin out! http://www.albinoblacksheep.com/flash/zoomquilt

Also good video!!! http://www.youtube.com/watch?v=6O6x_m4zvFs

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InvisibleTimmiTM
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Registered: 03/23/10
Posts: 5,303
Loc: Victoria Flag
Re: "sub-strain" [Re: FuckUp Eddy]
    #17575965 - 01/19/13 10:00 PM (11 years, 3 months ago)

The filamentous (thread-like) cells of a fungus are referred to as hyphae. When hyphae bundle together and grow to form a vegetative structure (the fluffy white threads that grow over a substrate) they are referred to as mycelium. The mycelium will aggregate and differentiate to form a fruit body (the mushroom). It's the same hyphae that make up both mycelium and mushroom, but exhibiting different forms of growth. Just as the cells of your body differentiated to grow into different organs.


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"Reality leaves a lot to the imagination" ~ John Lennon

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