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Invisible2Experimental
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Registered: 01/15/03
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Bleach/lime bath
    #1750794 - 07/26/03 03:36 PM (13 years, 4 months ago)

Can anyone give me correct times for soaking straw? Heres what Ive heard:

Pre soak straw for 1 hour, then move onto the bleach/lime bath, with boiling water... but my question is how long does it need to be in there to pasturize, and how long to drain it before spawning. If anyone can find the post where hippie3 gives the exact way to do it from stamets book, I will be very glad and grateful, or if anyone can tell me how its done i would be the same. Oh, and also the water to lime/bleach ratios if you know it off the top of your head... if not, with a search i could find those, but the first thing I asked I dident find with a search.. Oh well, off to take a long needed nap. Thanks in advance : )


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OfflineMrUnorthadox
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Re: Bleach/lime bath [Re: 2Experimental]
    #1750940 - 07/26/03 05:06 PM (13 years, 4 months ago)

a little bird told me to let it soak over night!?!----one


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InvisibleI_Stain_Blue
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Re: Bleach/lime bath [Re: MrUnorthadox]
    #1752159 - 07/27/03 02:46 AM (13 years, 4 months ago)

also interested.... anyone?


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InvisibleATWAR
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Re: Bleach/lime bath [Re: 2Experimental]
    #1752397 - 07/27/03 05:49 AM (13 years, 4 months ago)

Quote:

the exact way to do it from stamets book




How about go to the bookstore? Buy one of the books! There is lots of good info in there, and nice pictures of commercial mushroom cultivation methods.  :grin: :thumbup:

I will look it up when I get home for you, but I don't know if it is in GGMM or TMC. I only have GGMM anymore, it has some mention of different pasteurization techniques. But, I can shed some light on it for now:

I would soak the straw for a long time. Perhaps 12-24 hours prior to allow full saturation. I am not sure if boiling water would be good, as it is much hotter than actual pasteurization temperatures. Keep it around 160, not to exceed 170F. Duration should be an hour  , then its ready to spawn when cool. Ratios of bleach and lime are questionable. I assume you would have to test the PH, rather than add a pre-determined amount of lime. Bleach can be accurately measured but I do not know the ratio.

I will check GGMM later today if no one can help shed further light on this subject.. 


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InvisibleATWAR
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Re: Bleach/lime bath [Re: ATWAR]
    #1752817 - 07/27/03 01:25 PM (13 years, 4 months ago)

Ok, here it is. There was no method of bleach and lime together, but separate pasteurization methods.

Hydrated Lime Bath: two to four pounds of hydrated lime are added to 50 gallons of water. The PH will soar to around 9.5+. The straw is then submerged overnight, then drained and inoculated.

Bleach Bath Method: Stamets recommends 3-4 cups bleach for 50 gallons of water (5.25% hypochlorite household bleach). Straw is soaked for a minimum of 4, and no more than 12 hours.

He also states that if full colonization does not occur within a week of inoculation, contamination usually sets in with these methods. I see no reason why you couldnt combine the two methods.


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Invisible2Experimental
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Re: Bleach/lime bath [Re: ATWAR]
    #1753241 - 07/27/03 04:41 PM (13 years, 4 months ago)

thanks man, im sure others will find this usefull also.


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