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Invisiblenot2sure
journeyman
Registered: 02/02/02
Posts: 60
Loc: west coast
help ID this...
    #1749473 - 07/25/03 10:48 PM (13 years, 4 months ago)

swim keeps getting some contam that she cant positively ID. it starts bright white, but not ropey, almost like cobweb, but brigher. It gets fuzzy almost like peach fuzz, then turns green on the under side. it does smell funny, almost bacterial, and every spawn she has tried with rye or CC gets it. shes tried sterilizing, pasturizing poo and straw. she bought a hepa filter for that room on 24/7 and a cool mist with h202 in the chamber. she recently cleaned the entire room, fruiting chamber, incubator etc... it seems its the poo that is the problem, because the jars are clean, healthy, then she tries to spawn and a week later it smells funny, then it starts growin white fuzz etc...

is this just trich? how can she help this?

tia

not2sure


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The above statement is fictitious and for entertainment purposes only. I do not use or condone the use of illegal substances, nor do I use or condone the use of legal substances in an illegal manner.


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OfflineAnnoA
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Re: help ID this... [Re: not2sure]
    #1750157 - 07/26/03 05:09 AM (13 years, 4 months ago)

It?s some kind of green mold, either Trichoderma or Aspergillus or Penicillum. Impossible to say without a microscopical examination.


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Invisiblenot2sure
journeyman
Registered: 02/02/02
Posts: 60
Loc: west coast
Re: help ID this... [Re: Anno]
    #1750258 - 07/26/03 08:00 AM (13 years, 4 months ago)

how can she fix this? does oust work at all? so far she has had 0% success in the summer months with any tek, although she tries to be as clean as possible. gloves alcohol, lysol, wipes bleach water, hepa etc...shes spent more money trying to keep clean than anything else at this point!!!

thanks for the reply!


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The above statement is fictitious and for entertainment purposes only. I do not use or condone the use of illegal substances, nor do I use or condone the use of legal substances in an illegal manner.


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InvisibleMOTH
Wild Woman
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Registered: 06/06/03
Posts: 23,359
Loc: In the jungle
Re: help ID this... [Re: not2sure]
    #1765762 - 07/31/03 01:11 PM (13 years, 4 months ago)

Yeah, its pretty fucked up.  My kitten is as sanitary as possible and she just recently lost 3 jars to that green shit, all of the same strain.  Naturally, she isn't too happy about it and discouraged. :frown:  I mean seriously, things were locked down tight at innoculation.  I guess she'll just have to figure out what she's doing wrong. 


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OfflineSeussA
Error: divide byzero

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Registered: 04/27/01
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Re: help ID this... [Re: MOTH]
    #1765911 - 07/31/03 01:59 PM (13 years, 4 months ago)

When you have white fuzz that turns green with age, the green color is usually from the spores that the white fuzz produces. These spores are very small and become airborn easily.

Try to figure out where the contamination is coming from. Does it show up in the jars while they are colonizing. Does it only show up after the cakes have been removed from the jars? In the first case, the spores are being introduced before sterilization or during innoculation. In the second case, the problem is with the terrarium, humidifier, air exchange, or something related to the environment.

Do not inoculate one of your jars the next time, but treat it just like all the others in every other respect. If this jar contaminates, you know that the problem is coming before you inoculate.

A few things to remember in general about contamination from spores:

1) spores have weight and are pulled down by gravity. Almost 90% of all airborn spores will be within one foot of the ground. Be sure you are working on a counter or table top which is several feet off the floor.

2) spores are light and can be stirred up and ride air currents. The more you move around, or have fans blowing, the more spores you are going to stir up from the floor to fall onto you work.

3) turn off all fans, air conditioners, heaters, etc a few hours before you work. Close all windows and do everything you can to minimize drafts while you work.

4) your own body is a great place for spores to hide. Do not wear long sleeve shirts while working. Wear clean clothes and take a shower to clean yourself before hand. Use a lot of soap and hot water to clean your hands and the entire length of your arms. Wear latex or vinyl gloves while working. Use a 70% alcohol solution to wipe your arms and gloved hands with before touching anything.

5) a glove box is worth its weight in gold when you have problems with contaminations from spores. Get a roll of plastic wrap and some tape and a cardboard box. Line the inside of the box with plastic wrap. Turn the box on its side and make a 'shower curtain' out of strips of plastic wrap where you hands will go in. You can even get fancy and add a window to look in through the top of the box (covered in plastic wrap of course). Total cost < $5.00. Wipe down the inside of the box with 70% alcohol (or spray the hell out of it with lysol) each time before you use it.

6) skip the oven tek... it simply does not work. Every test I have done shows the hot air lifts up floor bound spores and deposits them onto your work surface. Others (Anno) have verified these observations. (Actually, I verified his observations... credit him for figuring this one out)

7) presoak your substrate for 8 to 12 hours before you pressure cook it. Don't trust the microwave sterilization or boiling water will get hot enough to kill off your contamination. If you are having problems, a good pressure cooker or autoclave is a must. If you are using a PC, make sure that the seal is good and that the proper pressure is being created. If your PC has a weight to regulate pressure, be sure that you let the hot air be pushed out by the generated steam for several minutes before you place the rocker/weight on the nipple.

8) ensure that your syringe isn't contaminated. It does not good to keep everything sterile and then inject contaminted water into the system.

9) wipe down _everything_ you can think of with 70% alcohol solution, or with a dilute bleach solution... don't forget the incubator, terrarium, tubing, humidifier, etc...

10) clean your air filters in the furnace/AC blower if you have central air. Clean couches and chairs, curtrains, walls, any horizontal surfaces that can hold spores, the inside of your fridge, etc.

11) mix up smaller jars (if you are doing quart jars, or larger) Smaller jars colonize faster and give contamination less time to grow.


Good luck, and I hope this helps a bit.


--------------------
Just another spore in the wind.


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InvisibleMOTH
Wild Woman
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Registered: 06/06/03
Posts: 23,359
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Re: help ID this... [Re: Seuss]
    #1774285 - 08/03/03 12:24 AM (13 years, 4 months ago)

Wow, that helps alot Seuss!!  I'm sure I've heard all this before but for some reason while reading it all itemized and clear like that it really sank in.  Thanks for taking the time to write it up for us!  :smile: 

truly,
ShadeGirl


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OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
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Re: help ID this... [Re: MOTH]
    #1777730 - 08/04/03 11:00 AM (13 years, 4 months ago)

You are most welcome. Good luck.


--------------------
Just another spore in the wind.


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OfflineMAIA
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Re: help ID this... [Re: Seuss]
    #1777823 - 08/04/03 11:46 AM (13 years, 4 months ago)

Excellent post Seuss.
Thanks !!!

MAIA


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Spiritual being, living a human experience ... The Shroomery Mandala



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