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Offlinekhemfusion
battleaxewarrior

Registered: 06/24/03
Posts: 216
Loc: West Coast Canada
Last seen: 11 years, 5 months
Cake viability?
    #1749152 - 07/25/03 08:17 PM (13 years, 4 months ago)

Hello all, I have some cakes that I've PC'd on July 8th
They are half pints, with holes punched and taped over, and then another layer of aluminum foil over that, my question is:

Would they still be sterile if I haven't opened them? They've been sitting at about room temperature in the dark since the 8th (17 days ago)

I just finally got some syringes containing WATER ONLY today and am wondering, should I bake some new cakes, or will these ones be good enough and contamination free?


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Invisiblemicro
bunbun has a gungun
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Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: Cake viability? [Re: khemfusion]
    #1749222 - 07/25/03 08:43 PM (13 years, 4 months ago)

If they are contaminated you should be able to tell. I'd be more concerned about them drying out if the lids were not on tight, but if they aren't dry they should be o.k.

I've inoculated stuff over a month after they were p.c.'ed and haven't had a problem, but if you're concerned and don't have enough inoculum better safe than sorry, IMO.

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OfflineSomeMycologist
some guy
Registered: 12/22/02
Posts: 203
Loc: Mycoland
Last seen: 12 years, 9 months
Re: Cake viability? [Re: micro]
    #1749246 - 07/25/03 08:55 PM (13 years, 4 months ago)

I wouldnt use the same jars again because you should aim in making the incubation process as short as possible since the factor of contamination sets in. If the jars are 17 days old and incubation takes About 20 days then thats about 40 days total. they might not be contaminated now but i wouldnt bet on them being good 10 days down the line. An option would be to sterilize the jars again for a short time to kill of any germinating bacteria but you run the risk of having the jars dry out. If i were you id start a new batch of jars. its cheaper then wasting the syringes youve been waiting so long for. Good luck man



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Offlinekhemfusion
battleaxewarrior

Registered: 06/24/03
Posts: 216
Loc: West Coast Canada
Last seen: 11 years, 5 months
Re: Cake viability? [Re: SomeMycologist]
    #1749263 - 07/25/03 09:02 PM (13 years, 4 months ago)

Well, it's only 5 jars, and I have a fair amount of innoculum, I think what will be best for me to do is to make another fresh batch, and then innoc these current jars and wait on the results, there's two options with them, fail or succeed, so we'll see what path they take :smile:

When sterilizing a glovebox and innoculating inside one, do I just have to wipe the needle down with alcohol between stabs into a cake? I don't know if it's possible to have flame sterilization inside one, I have a butane torch lighter and thats about it


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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