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OfflineTheUnknownPoet
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hepa filtered cleanroom
    #17483660 - 01/02/13 12:41 AM (8 years, 10 months ago)

sorry 4 wrong section.
http://hackaday.com/2010/03/09/add-a-clean-room-to-your-workshop-2/
would this work for mycology? theres obviously turbulent air inside the bok but that shouldnt matter as long as its clean and positive pressure, right?


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OfflinePussyFart
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Re: hepa filtered cleanroom [Re: TheUnknownPoet]
    #17483710 - 01/02/13 12:48 AM (8 years, 10 months ago)

That air would not be clean enough given that filter.

If you cannot buy/build a TRUE Laminar Flow Hood, the absolute next best thing is a SAB(Still Air Box).


Edited by PussyFart (01/02/13 12:49 AM)


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OfflineTheUnknownPoet
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Re: hepa filtered cleanroom [Re: PussyFart]
    #17483882 - 01/02/13 01:26 AM (8 years, 10 months ago)

assuming i has a hepa filter to put in there. i cant get a laminar flow filter, but i have a 99.999% one, only its just not laminar


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OfflinePussyFart
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Re: hepa filtered cleanroom [Re: TheUnknownPoet]
    #17483941 - 01/02/13 01:41 AM (8 years, 10 months ago)

Someone brings this up every week it seems, and it always ends the same.

Just build a SAB. Its a clear tub with 2 arm hols, and better than any contraption you could possibly build that does not provide a true laminar flow with sterile air.

The next best thing to sterile air, is still air.


Edited by PussyFart (01/02/13 01:41 AM)


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OfflineTheUnknownPoet
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Re: hepa filtered cleanroom [Re: PussyFart]
    #17484103 - 01/02/13 02:28 AM (8 years, 10 months ago)

Quote:

Notahacker420 said:
Someone brings this up every week it seems, and it always ends the same.

Just build a SAB. Its a clear tub with 2 arm hols, and better than any contraption you could possibly build that does not provide a true laminar flow with sterile air.

The next best thing to sterile air, is still air.



building a sab is out of the question.


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OfflinePussyFart
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Re: hepa filtered cleanroom [Re: TheUnknownPoet]
    #17484164 - 01/02/13 02:58 AM (8 years, 10 months ago)

Quote:

TheUnknownPoet said:
Quote:

Notahacker420 said:
Someone brings this up every week it seems, and it always ends the same.

Just build a SAB. Its a clear tub with 2 arm hols, and better than any contraption you could possibly build that does not provide a true laminar flow with sterile air.

The next best thing to sterile air, is still air.



building a sab is out of the question.



Why if I may ask?


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OfflineKizzle
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Re: hepa filtered cleanroom [Re: PussyFart]
    #17484508 - 01/02/13 04:26 AM (8 years, 10 months ago)

What I would suggest if you wanted to go such a route would be to turn it off when it's actually in use to eliminate the turbulence and allow for any remaining contaminants to settle so they're not blowing into your cultures/jars, but then you're basically just using a SAB anyway.


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InvisibleFungal growth
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Re: hepa filtered cleanroom [Re: Kizzle]
    #17484883 - 01/02/13 08:30 AM (8 years, 10 months ago)

considering this hobby is largely practiced by mcguyver-type stoners and some very thrifty people, i'm thinking if your idea worked op, no one would use laminar flow hoods.


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OfflineTheCyndicate
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Re: hepa filtered cleanroom [Re: Fungal growth]
    #17484919 - 01/02/13 08:53 AM (8 years, 10 months ago)

Quote:

Fungal growth said:
considering this hobby is largely practiced by mcguyver-type stoners and some very thrifty people, i'm thinking if your idea worked op, no one would use laminar flow hoods.




I use the one I built ....SEE



Cyn


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OfflineRogerRabbitM
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Re: hepa filtered cleanroom [Re: TheUnknownPoet]
    #17485050 - 01/02/13 09:42 AM (8 years, 10 months ago)

Quote:

TheUnknownPoet said:

building a sab is out of the question.




It sounds like you're screwed then.

That thing won't filter fine enough for mycology, while producing turbulence at the same time, so it's going to be pure fail.

By the way, how does one stick his hands into that box to use the soldering iron? :shrug:
RR


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OfflineTheCyndicate
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Re: hepa filtered cleanroom [Re: RogerRabbit]
    #17485056 - 01/02/13 09:43 AM (8 years, 10 months ago)

Quote:

RogerRabbit said:
Quote:

TheUnknownPoet said:

building a sab is out of the question.




It sounds like you're screwed then.

That thing won't filter fine enough for mycology, while producing turbulence at the same time, so it's going to be pure fail.

By the way, how does one stick his hands into that box to use the soldering iron? :shrug:
RR




I was wondering the same thing RR.


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OfflineTheUnknownPoet
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Re: hepa filtered cleanroom [Re: TheCyndicate]
    #17501338 - 01/05/13 12:56 PM (8 years, 10 months ago)

I thought this operated on the principle that it will put filtered air into the boc, and since it's positive pressure at all times, nothing can get in... sure the airflow may be turbulent, but if it's 99.99% HEPA filtered at 0.3 microns, it'll be clean enough + positive pressure means things are blwoing OUT, not IN.


Wont this work, in theory? COme on guys, there's got to be a different way to do this than a laminar flowhood. How do HEPA cleanrooms work? Can't I have a mini cleanroom?


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Re: hepa filtered cleanroom [Re: TheUnknownPoet]
    #17501426 - 01/05/13 01:25 PM (8 years, 10 months ago)

Built my SAB for 20$ supplies from local hardware store. I don't get how this could be out of the question for anyone.


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Re: hepa filtered cleanroom [Re: Trippy_Penguin]
    #17501436 - 01/05/13 01:27 PM (8 years, 10 months ago)

Quote:

petersodm said:
Built my SAB for 20$ supplies from local hardware store. I don't get how this could be out of the question for anyone.




:whathesaid:


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Re: hepa filtered cleanroom [Re: TheUnknownPoet]
    #17501437 - 01/05/13 01:28 PM (8 years, 10 months ago)

Well around here youll encounter hive mind. I have some input, but cannot guarantee any degree of success.

Get a propane powered mosquito fogger. Make sure its new and unused. You will not be putting insect repellent in it. Buy propylene glycol. You can get it on the net, hardware stores, and pharmacies.

Propylene glycol when vaporized is 99.999 percent effective in killing bacteria, mold, and virii. Im not sure about endospores.....

Anyway plastic decontamination chambers use these type of vaporizers when they blast blast you with white vapor before you enter, and after you exit a bio lab.


Pair this with your hepa filtered room. Blast it clean before doing work in it.

Its possible, that if you glove up, suit up, mask up, wear a hood, lab booties, Decontam before entering the room, that you might have a success rate.

After all this, wouldnt you just rather have a flow hood? Its a much more elegant solution.

I use a medium sized SAB. Its a bit of a PITA to work in, but it works well, and im getting better at using it.


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Re: hepa filtered cleanroom [Re: Sillyputty67]
    #17502118 - 01/05/13 04:10 PM (8 years, 10 months ago)

Quote:

After all this, wouldnt you just rather have a flow hood? Its a much more elegant solution.



right? how mucth time and cash would a cleanroom cost?
cuz a flowhood can be built for under $500, easy.


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OfflineTheUnknownPoet
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Re: hepa filtered cleanroom [Re: Fungal growth]
    #17502292 - 01/05/13 04:43 PM (8 years, 10 months ago)

Oh my goodness guys. There's a specific reason I am asking for this, i obviously KNOW that a flowhood is better, but i don't have NOR CAN I GET  a flowhood. PLEASE, stay on topic and let me know if this would work as well. i understand there are better ways, but that does not necessarily mean I can use such better ways.

Last i checked, Cleanrooms were not laminar flow, and all i have access to is hepa filters which are NOT laminar flow, and i need to make this work somehow. there is no other option.

A am using a still air box currently, and i fucking hate it.


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Re: hepa filtered cleanroom [Re: TheUnknownPoet]
    #17502302 - 01/05/13 04:45 PM (8 years, 10 months ago)

i doubt you will be able to build a lab grade "clean room" in your house unless you got a huge bankroll. so flow hood is winner here


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OfflinePussyFart
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Re: hepa filtered cleanroom [Re: TheUnknownPoet]
    #17502310 - 01/05/13 04:47 PM (8 years, 10 months ago)

Quote:

TheUnknownPoet said:
there is no other option.




Quote:

TheUnknownPoet said:
A am using a still air box currently, and i fucking hate it.




The absolute next best thing to a real flow hood is still air.

Get a bigger tub and call it a day, keep it simple bro.


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Offlinecloudpersona
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Re: hepa filtered cleanroom [Re: PussyFart]
    #17502322 - 01/05/13 04:49 PM (8 years, 10 months ago)

Quote:

Notahacker420 said:
keep it simple bro.



QFT


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Re: hepa filtered cleanroom [Re: TheUnknownPoet]
    #17502358 - 01/05/13 04:56 PM (8 years, 10 months ago)

I've used a positive pressure box many times, mine worked fine.

I switched to a SAB because it's actually easier.

With a SAB, I can flame sterilize outside the box and easily get the needle back in the SAB.

Although that set up could possibly work, it's much easier to just use a SAB. That setup will still have the same inconveniences as a SAB without adding a new level of sterility.

I feel ya on the SAB sucking, but, it really is the best option until you get a flowhood.


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Re: hepa filtered cleanroom [Re: TheUnknownPoet]
    #17502387 - 01/05/13 05:01 PM (8 years, 10 months ago)

Quote:

TheUnknownPoet said:
Oh my goodness guys. There's a specific reason I am asking for this, i obviously KNOW that a flowhood is better, but i don't have NOR CAN I GET  a flowhood. PLEASE, stay on topic and let me know if this would work as well. i understand there are better ways, but that does not necessarily mean I can use such better ways.

Last i checked, Cleanrooms were not laminar flow, and all i have access to is hepa filters which are NOT laminar flow, and i need to make this work somehow. there is no other option.

A am using a still air box currently, and i fucking hate it.




It sounds like your looking for a more economical step up between your SAB and a proper flow hood. The short answer is that there isnt one.

keep scouring ebay/CL for lab liquidation companies and try to find a deal that way, or DIY a flow hood.
I think i picked mine up from ebay for under $300 and its been money well spent.

from wikip to clarify why a cleanroom doesnt need laminar flow-
A cleanroom or clean room is an environment, typically used in manufacturing or scientific research, that has a low level of environmental pollutants such as dust, airborne microbes, aerosol particles and chemical vapors. More accurately, a cleanroom has a controlled level of contamination that is specified by the number of particles per cubic meter at a specified particle size. To give perspective, the ambient air outside in a typical urban environment contains 35,000,000 particles per cubic meter in the size range 0.5 μm and larger in diameter, corresponding to an ISO 9 cleanroom, while an ISO 1 cleanroom allows no particles in that size range and only 12 particles per cubic meter of 0.3 μm and smaller.


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InvisibleFungal growth
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Re: hepa filtered cleanroom [Re: hoobatech]
    #17504711 - 01/06/13 01:24 AM (8 years, 10 months ago)

allz i'm saying is if you can bankroll a cleanroom what's the big deal with a flowhood?
but anyway, what sort of hepa filters do you have?
are you planning on sourcing all supplies locally?


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Re: hepa filtered cleanroom [Re: Fungal growth]
    #17505515 - 01/06/13 06:29 AM (8 years, 10 months ago)

Well you could make self healing ports on your lids and stuff poly fill in. Theres teks  in it if you look


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