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SpitballJedi
Ancient Astronaut
Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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I've used a positive pressure box many times, mine worked fine.
I switched to a SAB because it's actually easier.
With a SAB, I can flame sterilize outside the box and easily get the needle back in the SAB.
Although that set up could possibly work, it's much easier to just use a SAB. That setup will still have the same inconveniences as a SAB without adding a new level of sterility.
I feel ya on the SAB sucking, but, it really is the best option until you get a flowhood.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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hoobatech
Registered: 08/16/04
Posts: 285
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Quote:
TheUnknownPoet said: Oh my goodness guys. There's a specific reason I am asking for this, i obviously KNOW that a flowhood is better, but i don't have NOR CAN I GET a flowhood. PLEASE, stay on topic and let me know if this would work as well. i understand there are better ways, but that does not necessarily mean I can use such better ways.
Last i checked, Cleanrooms were not laminar flow, and all i have access to is hepa filters which are NOT laminar flow, and i need to make this work somehow. there is no other option.
A am using a still air box currently, and i fucking hate it.
It sounds like your looking for a more economical step up between your SAB and a proper flow hood. The short answer is that there isnt one.
keep scouring ebay/CL for lab liquidation companies and try to find a deal that way, or DIY a flow hood. I think i picked mine up from ebay for under $300 and its been money well spent.
from wikip to clarify why a cleanroom doesnt need laminar flow- A cleanroom or clean room is an environment, typically used in manufacturing or scientific research, that has a low level of environmental pollutants such as dust, airborne microbes, aerosol particles and chemical vapors. More accurately, a cleanroom has a controlled level of contamination that is specified by the number of particles per cubic meter at a specified particle size. To give perspective, the ambient air outside in a typical urban environment contains 35,000,000 particles per cubic meter in the size range 0.5 μm and larger in diameter, corresponding to an ISO 9 cleanroom, while an ISO 1 cleanroom allows no particles in that size range and only 12 particles per cubic meter of 0.3 μm and smaller.
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Fungal growth
Lootinint
Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
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Re: hepa filtered cleanroom [Re: hoobatech]
#17504711 - 01/05/13 11:24 PM (11 years, 3 months ago) |
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allz i'm saying is if you can bankroll a cleanroom what's the big deal with a flowhood? but anyway, what sort of hepa filters do you have? are you planning on sourcing all supplies locally?
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Crazymofomonk
philosopher
Registered: 11/19/12
Posts: 102
Loc: west coast
Last seen: 9 years, 11 months
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Well you could make self healing ports on your lids and stuff poly fill in. Theres teks in it if you look
-------------------- don't lose yourself
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