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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 4 months
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Adding yeast Nutrient and 5-HTP to LC
#17480351 - 01/01/13 10:33 AM (11 years, 4 months ago) |
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(cya all information contained in this post or any other is purely fictional)
I have been using 5-htp as an additive during watering on my fully colonized sub. Results have shown a possible leap in psychoactives, but I believe the method I have been using is too crude to actually prove one way or another. I am curious as to what effect 5-htp would have on the final product if the mycelium is introduced and continuously exposed beginning at germination. Also I have been curious about adding yeast nutrient(wine making) to the LC prior to germination to see if growth can be sped up, or increase the health of the mycelium.
For the experiment I am using 12 1-pint jars with self healing injection ports.
6 jars will be Albino A+ of these 2 will be controls (no additives) 2 will have yeast NUTE at .5tsp/gal and 1tsp/gal 2 will have 5-HTP at 100mg/400ml and 50mg/400ml
6 jars will be PES Hawaiian of these 2 will be controls (no additives) 2 will have yeast NUTE at .5tsp/gal and 1tsp/gal 2 will have 5-HTP at 100mg/400ml and 50mg/400ml
Each of the jars contains appx 3tsp or less of light karo syrup.
Each of the values for the 5-HTP and Yeast NUTE are a combination of "best Guess" and common use.
I am looking forward to any questions comments or bright ideas.
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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cloudpersona
I don't even...
Registered: 12/17/11
Posts: 1,285
Last seen: 6 years, 6 months
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: Zenlykos]
#17480370 - 01/01/13 10:39 AM (11 years, 4 months ago) |
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-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” – Terence McKenna “If the words ‘life, liberty, and the pursuit of happiness’ don’t include the right to experiment with your own consciousness, then the Declaration of Independence isn’t worth the hemp it was written on.” -Terence McKenna
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 4 months
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: cloudpersona]
#17480420 - 01/01/13 10:56 AM (11 years, 4 months ago) |
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I've spent the better part of the year straining my eyes on the forums, but so far there are too many different opinions on the matter. to many variables and not enough controls. My results are going to be less than exact since i'm using multi-spore. But isn't it worth trying?
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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cloudpersona
I don't even...
Registered: 12/17/11
Posts: 1,285
Last seen: 6 years, 6 months
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: Zenlykos]
#17480475 - 01/01/13 11:10 AM (11 years, 4 months ago) |
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No. Especially not if you're using multi-spore. And it's a waste of time unless you have a GCMS, to measure content.
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” – Terence McKenna “If the words ‘life, liberty, and the pursuit of happiness’ don’t include the right to experiment with your own consciousness, then the Declaration of Independence isn’t worth the hemp it was written on.” -Terence McKenna
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 4 months
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: cloudpersona]
#17480526 - 01/01/13 11:23 AM (11 years, 4 months ago) |
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Well regardless I am going to press on. I am not looking for an all defining answer as to a precise xx.xx% increase in growth speed/mass and +/-xx.xx% increase in psychoactives. But a GCMS would be helpful. Hopefully this experiment will yield a completely unexpected result which would open the door for even more questions. My kind of party.
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,866
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: Zenlykos]
#17482143 - 01/01/13 05:35 PM (11 years, 4 months ago) |
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Mycelium doesn't simply absorb everything it's exposed to. There are specific chemicals it can absorb, mainly chemicals produced after it externally digests a substrate. It's unlikely any 5-HTP would be absorbed in the first place.
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 4 months
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: Kizzle]
#17482220 - 01/01/13 05:54 PM (11 years, 4 months ago) |
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I understand that mycelium does not absorb everything you expose it too, but I am hoping that if it cannot in fact absorb the full 5-htp molecule, it might be able to absorb whatever it breaks down into during the PC cycle. I'd love to get an idea of what 5-HTP breaks down into. but alas I do not have the equipment for it.
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,866
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: Zenlykos]
#17482274 - 01/01/13 06:11 PM (11 years, 4 months ago) |
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I doubt it would be able to digest much of anything in an LC. Mycelium generally has to be to colonize a substrate first to digest it so it can stay in contact with the material.
It's also possible even in a situation where it could digest it, it may not have to enzymes so do so and the product if it can would probably be something simple that would be used for purposes other than producing actives.
Not that there's anything wrong with experimenting with it. It's the only way to know anything for certain, but it has been tried many times.
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 4 months
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: Kizzle]
#17482336 - 01/01/13 06:27 PM (11 years, 4 months ago) |
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I assumed as much, that the mycelium would need to be in constant contact, but I believe that since the 5-htp would break down into smaller molecules, it could be absorbed as easily as the sugars in the LC suspension. Also, since fluid will be taken from the LC jar to inoculate the substrate, the 5-htp in suspension will also be transferred over and will be available to the colonizing mycelium immediately. and even if the chemicals are used for any other purpose than to produce actives, maybe I will stumble upon something new that was completely unexpected. Maybe create a mutation. who knows...
and I know so many people have done this before, But I'm hoping that having first hand knowledge will shed light on areas that I might not even be concerned about yet or have no knowledge of.
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 4 months
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: Zenlykos]
#17488001 - 01/02/13 06:57 PM (11 years, 4 months ago) |
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So the LC jars have been Inoculated. Pics to follow. both the 5-htp and yeast nutrient cause varying levels of color change and effects. will report soon.
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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funguy666
Professor
Registered: 12/10/12
Posts: 91
Loc:
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: Zenlykos]
#17496753 - 01/04/13 12:41 PM (11 years, 4 months ago) |
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Can't wait!!
-------------------- Come with me, and you'll be, in a world of pure imagination
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 4 months
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: funguy666]
#17504631 - 01/05/13 10:53 PM (11 years, 4 months ago) |
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Sorry about the wait, computer was down for a bit, and I got my hands on some moon rocks so I was a little too high to care. anyways from first to last: full photo, regular LC, 50mg 5-HTP, .5tsp/gal Yeast nute, 100mg 5-htp, 1tsp/gal yeast nute, full photo.

You can immediately notice a big color shift with the higher concentrations, and also funny enough the jars with the yeast nutrient continued to bubble after the others had stopped. also they were the last to cool. I'll check after the test is done, and see if the specific gravity could have caused this, or if the yeast nutrient reacted with the water. No clue but I will keep y'all informed.
Its been a few days since I inoculated these jars and already they have germinated. incubation temp is appx 84*f. The yeast nute jars are showing a curious formation of what I would think is mycelium, but it is waaay to soon for that to be possible. It is probly just the yeast nutrient floating around. I will update as soon as I know for sure.
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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budmanman
OTD Masterbater



Registered: 02/07/07
Posts: 18,259
Loc: PNW
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: Zenlykos]
#17504938 - 01/06/13 12:23 AM (11 years, 4 months ago) |
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You forgot your marble for stiring and you forgot to finish making your lids.
-------------------- Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal. And I am mentally unstable.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: budmanman]
#17505503 - 01/06/13 04:21 AM (11 years, 4 months ago) |
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Quote:
incubation temp is appx 84*f.
That's going to encourage competitor organisms and stunt mushroom mycelium. Drop the temp a few degrees. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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morbiddoctor
Frilly fungal fruiter



Registered: 07/05/09
Posts: 1,021
Loc: seattle, wa
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: RogerRabbit]
#17507326 - 01/06/13 02:14 PM (11 years, 4 months ago) |
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you just fermented yeast in a jar. The jars were warm because they were actively fermenting on the sugar that you added to the LC. if there is myc in there at all, it is cohabitating with yeast.
-------------------- Trade list Updated 12/20/15 Ranco temperature controller and Wagner steamer for pasteurization in trade list! I'm looking for eyecatching, colorful, unique and interesting fungi. Not limited to edible or medicinal. Print them when you're on foray and pm me please!
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 4 months
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: budmanman]
#17507411 - 01/06/13 02:33 PM (11 years, 4 months ago) |
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Quote:
budmanman said: You forgot your marble for stiring and you forgot to finish making your lids.
I do not use a marble or anything for stirring. there is plenty of room left to just shake up the mycelium. and my lids are done. its just the way I do it. dont ask, it works fine for me. thanks
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 4 months
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: Zenlykos]
#17507416 - 01/06/13 02:34 PM (11 years, 4 months ago) |
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Thanks RR will do. going to drop to 80*f
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 4 months
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: morbiddoctor]
#17507442 - 01/06/13 02:38 PM (11 years, 4 months ago) |
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Quote:
morbiddoctor said: you just fermented yeast in a jar. The jars were warm because they were actively fermenting on the sugar that you added to the LC. if there is myc in there at all, it is cohabitating with yeast.
Sorry bro, but no. I used nutrient that is intended to be used on yeast, not nutritional yeast like you are thinking of. also the intense heat of PC'ing would have killed the yeast, and unless I had a jar packed to the top with yeast and sugar, it could not and would not produce that much noticeable heat.
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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morbiddoctor
Frilly fungal fruiter



Registered: 07/05/09
Posts: 1,021
Loc: seattle, wa
Last seen: 2 years, 8 months
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: Zenlykos]
#17511407 - 01/07/13 10:37 AM (11 years, 4 months ago) |
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Quote:
Zenlykos said:
Quote:
morbiddoctor said: you just fermented yeast in a jar. The jars were warm because they were actively fermenting on the sugar that you added to the LC. if there is myc in there at all, it is cohabitating with yeast.
Sorry bro, but no. I used nutrient that is intended to be used on yeast, not nutritional yeast like you are thinking of. also the intense heat of PC'ing would have killed the yeast, and unless I had a jar packed to the top with yeast and sugar, it could not and would not produce that much noticeable heat.
ah sorry about that guess I misread.
-------------------- Trade list Updated 12/20/15 Ranco temperature controller and Wagner steamer for pasteurization in trade list! I'm looking for eyecatching, colorful, unique and interesting fungi. Not limited to edible or medicinal. Print them when you're on foray and pm me please!
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 4 months
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Re: Adding yeast Nutrient and 5-HTP to LC [Re: morbiddoctor]
#17513472 - 01/07/13 06:25 PM (11 years, 4 months ago) |
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s'all good.
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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