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InvisibleDiploidM
Cuban

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Registered: 01/09/03
Posts: 19,274
Loc: Rabbit Hole
Trusted Cultivator
I'm all shook up!
    #1747301 - 07/25/03 09:17 AM (13 years, 4 months ago)

I've used rye, birdseed, millet, popcorn, and assorted other stuff successfully for some time. Sometimes I shake the grain immediately after inoculation and other times I let it sit for a few days until I see mycelium before shaking. I've been unable to produce consistent results. Sometimes the early shaking seems to delay the initial growth, other times it seems to speed up.

Maybe the varying factor is the spore concentration in the syringe. I'm thinking that a dilute solution would put a long distance between any two haploid hyphae looking for each other if immediately shaken. On the other hand, a very dark solution could put so many spores in the jar that two compatible haploid hyphae never have very far to go to find each other even when immediate shaking spreads them out.

Ideas, comments, suggestions?

TIA

-Diploid


--------------------
Republican Values:

1) You can't get married to your spouse who is the same sex as you.
2) You can't have an abortion no matter how much you don't want a child.
3) You can't have a certain plant in your possession or you'll get locked up with a rapist and a murderer.

4) We need a smaller, less-intrusive government.


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OfflineQuadrant6
Blood Red Eyes

Registered: 07/24/03
Posts: 89
Loc: Holland
Last seen: 7 years, 10 months
Re: I'm all shook up! [Re: Diploid]
    #1747442 - 07/25/03 10:15 AM (13 years, 4 months ago)

I agree. Sometimes with dilluted syringes, it will take a little longer at first, but when i use a real dark syringe i will see growth immediately. I innoculated 1 quart of popcorn with 5 cc's of a dark f+ syringe and it was fully colonized in 2 days. Crazy shit.


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"Time is the substance from which I am made. Time is a river which carries me along, but I am the river; it is a tiger that devours me, but I am the tiger; it is a fire that consumes me, but I am the fire."
Jorge Luis Borges


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OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
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Re: I'm all shook up! [Re: Diploid]
    #1747447 - 07/25/03 10:17 AM (13 years, 4 months ago)

I think germination takes the time that it takes regardless of if you shake or not after injection.... the first shake after initial germination is more important

ie: how long you wait before your first shake once mycelium has started to grow!!


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-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


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InvisibleDiploidM
Cuban

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Registered: 01/09/03
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Re: I'm all shook up! [Re: PooPs]
    #1747518 - 07/25/03 10:47 AM (13 years, 4 months ago)

Quote:

I think germination takes the time that it takes regardless of if you shake or not




Germination, yes, I agree. But even when all the spores germinate, at least two haploid hyphae have to meet before the mycelium becomes diploid and begins to up the vegitative growth rate. If the I shake the jar immediately after inoculating, then the haploid hyphae have a longer distance to cover before they meet. If I don't shake, the spores will be concentrated in an smaller area so the distances are shorter.

This makes sense to me. The problem is that my experiments with shaking and not shaking are inconclusive. I can't seem to get any consistent results. Some shaken jars go fast, others don't; same with non-shaken jars.

-Diploid


--------------------
Republican Values:

1) You can't get married to your spouse who is the same sex as you.
2) You can't have an abortion no matter how much you don't want a child.
3) You can't have a certain plant in your possession or you'll get locked up with a rapist and a murderer.

4) We need a smaller, less-intrusive government.


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OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
Loc: Stirrin up the pile!
Last seen: 7 years, 7 months
Re: I'm all shook up! [Re: Diploid]
    #1747560 - 07/25/03 11:16 AM (13 years, 4 months ago)

could be as simple as water content of each jar not being the same...

or with multispore you simply can't get 100 % consistent results!


--------------------


-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


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Offlinedickmcplentie
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Registered: 06/27/03
Posts: 75
Loc: Orions Belt at the moment
Last seen: 13 years, 4 months
Re: I'm all shook up! [Re: PooPs]
    #1747707 - 07/25/03 12:38 PM (13 years, 4 months ago)

A different way of looking at it:
What if the vegitative growth has begun to alter your frontal lobe?

I don't want to get all technical on you but, I think when the tall things with the flat tops pop up your supposed to eat em'  :wink: 


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The above statement has been a figment of my imagination. Niether the statement nor the writer is real, we are both fiction & should not be taken seriously in any way


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InvisibleDiploidM
Cuban

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Registered: 01/09/03
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Re: I'm all shook up! [Re: dickmcplentie]
    #1747757 - 07/25/03 12:59 PM (13 years, 4 months ago)

Quote:

A different way of looking at it:
What if the vegitative growth has begun to alter your frontal lobe?

I don't want to get all technical on you but, I think when the tall things with the flat tops pop up your supposed to eat em' 




OMG... Fugu, is that you?  :tongue:

-Diploid 


--------------------
Republican Values:

1) You can't get married to your spouse who is the same sex as you.
2) You can't have an abortion no matter how much you don't want a child.
3) You can't have a certain plant in your possession or you'll get locked up with a rapist and a murderer.

4) We need a smaller, less-intrusive government.


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: I'm all shook up! [Re: Diploid]
    #1747988 - 07/25/03 02:02 PM (13 years, 4 months ago)

IMHO You should wait until you see aproxamitly 15 to 25% colonization before shaking for the "first" time , then shake again around 70 to 80 % , then just let those suckers sit till birthing time .
Shaking is slightly harmful to the colony , it is best to let the colony really get a strong hold in the grain before disrupting its growth , letting it get strong will give it better chances of recovering between the shakes . Shaking too often can weaken the colony to the point where it is more likely to get contaminated , or not recover from the abuse .
Surgeons will not perform surgery on sick people , same concept applies to myc development .
Hope I made some sense with my analogy .?
Good Luck


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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Offlinedickmcplentie
Traveler
Registered: 06/27/03
Posts: 75
Loc: Orions Belt at the moment
Last seen: 13 years, 4 months
Re: I'm all shook up! [Re: tripndicular]
    #1748224 - 07/25/03 02:55 PM (13 years, 4 months ago)

No FUGU here... but i like him already


--------------------
The above statement has been a figment of my imagination. Niether the statement nor the writer is real, we are both fiction & should not be taken seriously in any way


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OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
Loc: Stirrin up the pile!
Last seen: 7 years, 7 months
Re: I'm all shook up! [Re: dickmcplentie]
    #1748254 - 07/25/03 03:07 PM (13 years, 4 months ago)

I usually try to shake only once.. but shake HARD.


--------------------


-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop for: ½ Pint Jars, Rye Grain, Wild Bird Seed

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