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OfflineProfessorPinHead
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Re: What type of commercial agar is most suitable? [Re: Terry M]
    #17488431 - 01/02/13 08:14 PM (11 years, 4 months ago)

Quote:

Terry M said:

Yes, most antibiotics break down under heat. That's why the one specific antibiotic used for agar is gentamicin sulfate. This IS stable during sterilization.






It still doesn't work all that well though. I've only dabbled with the stuff but it only slowed the bacteria that I was dealing with down.

This was helpful to get it to the next plate faster but didn't stop the growth of anything from my experience.

It's kind of like a cheat sheet on a test.

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OfflineJML
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Registered: 08/18/09
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Re: What type of commercial agar is most suitable? [Re: OICU812]
    #17488597 - 01/02/13 08:41 PM (11 years, 4 months ago)

Quote:

OICU812 said:
Quote:

JML said:
I use stamets antibiotic MEA. Fungi.com




Why antibiotic?



I kept getting bacterial contams. Thought I would used it just in case my syringes were bad. Has worked well so far


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OfflineTerry M
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Re: What type of commercial agar is most suitable? [Re: JML]
    #17488668 - 01/02/13 08:53 PM (11 years, 4 months ago)

Gentamicin only kills gram-negative bacteria. :frown:


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Liberté, égalité, humidité.

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InvisibleFischer
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I'm a teapot

Registered: 06/03/10
Posts: 565
Loc: Downunder
Re: What type of commercial agar is most suitable? [Re: Terry M]
    #17507716 - 01/06/13 03:40 PM (11 years, 4 months ago)

In case anyone's interested, here's TIC Gum's online store for small quantities (I presume one pound, from what I was told, but I'll let y'all know when I receive it)

https://qnet.e-quantum2k.com/~brite/Custom/1145/RETAIL/cgi-bin/nph-main.cgi

Comparatively exxy, but it's a hobby for me so it's not an issue.

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OfflineProfessorPinHead
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Re: What type of commercial agar is most suitable? [Re: Fischer]
    #17509183 - 01/06/13 08:37 PM (11 years, 4 months ago)

That's excellent. I'll keep my eye out to see how you like it.

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