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Zarotti
Stranger

Registered: 05/12/12
Posts: 314
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Can you always achieve Rhyzomorphic Myzelium when using Agar ?
#17412882 - 12/18/12 04:43 PM (11 years, 1 month ago) |
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Couple weeks ago dripped a few drops of a Spore Syringe on agar
->The No pour agar jar (No pour Agar Tek) showed multiple colonies with (only) cotton myzelium -no rhyzomorphic growth (as expected)
->Did the first transfer -6days old so far only thin/fuzzy/cotton myzelium visible
Q:---> If you keep on transfering the thickest,leading edge of your cotton agar myzelium will there be a time where rhyzomorpic growth appears ?
------------------------------------------------------------------- Just read this Thread about moisture in relevance to rhyzomorphic growth
http://www.shroomery.org/forums/showflat.php/Number/10561634#10561634
^ iS this representable ?
If your agar is wet -->fuzzy myzelium If its dry -->Rhyzomorphic thick myzelium ?
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: Zarotti]
#17412896 - 12/18/12 04:45 PM (11 years, 1 month ago) |
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use the search engine, RR states somewhere the less nutrients you use in agar the more rhiz you get
i dont know the recipe or nothing though
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ArmFromTheAbyss
Old Hand

Registered: 10/09/02
Posts: 1,368
Loc: Down here in Babylon
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: Zarotti]
#17413156 - 12/18/12 05:30 PM (11 years, 1 month ago) |
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It may take several transfers to see rhizomorphs.
Mycelium can be cottony on an agar dish only to become rhizomorphic when transferred to grain. Colonized grain can be cottony only to become rhizomorphic when spawned to a bulk sub. IMO, selecting random pieces of rhizomorphic mycelium is just as much of a crap-shoot as MS. The presence of a rhizomorph on a petri dish is a not a direct indication of a good fruiting or potent strain. I open my dishes in front of a flow hood to air them out and induce pinning. Then I select a good pin and clone it. Typically only 1 or 2 more transfers will give a monoculture of a fruiting strain.
The way RR does it is like taking an apple and planting all of its seeds, then waiting for them to grow into trees and seeing if they happened to produce good fruit. If you've got the time and it makes you happy then do it that way.
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SpitballJedi
Ancient Astronaut


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: ArmFromTheAbyss]
#17413218 - 12/18/12 05:41 PM (11 years, 1 month ago) |
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Quote:
ArmFromTheAbyss said: It may take several transfers to see rhizomorphs.
Mycelium can be cottony on an agar dish only to become rhizomorphic when transferred to grain. Colonized grain can be cottony only to become rhizomorphic when spawned to a bulk sub. IMO, selecting random pieces of rhizomorphic mycelium is just as much of a crap-shoot as MS. The presence of a rhizomorph on a petri dish is a not a direct indication of a good fruiting or potent strain. I open my dishes in front of a flow hood to air them out and induce pinning. Then I select a good pin and clone it. Typically only 1 or 2 more transfers will give a monoculture of a fruiting strain.
The way RR does it is like taking an apple and planting all of its seeds, then waiting for them to grow into trees and seeing if they happened to produce good fruit. If you've got the time and it makes you happy then do it that way.
You just blew my mind
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: ArmFromTheAbyss]
#17413356 - 12/18/12 06:02 PM (11 years, 1 month ago) |
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Quote:
ArmFromTheAbyss said: It may take several transfers to see rhizomorphs.
Mycelium can be cottony on an agar dish only to become rhizomorphic when transferred to grain. Colonized grain can be cottony only to become rhizomorphic when spawned to a bulk sub. IMO, selecting random pieces of rhizomorphic mycelium is just as much of a crap-shoot as MS. The presence of a rhizomorph on a petri dish is a not a direct indication of a good fruiting or potent strain.
Couldn't have said it better myself. I prefer planting all the seeds though
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HeadFood
Shroomer



Registered: 12/30/07
Posts: 390
Last seen: 11 years, 1 month
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: SpitballJedi]
#17413462 - 12/18/12 06:13 PM (11 years, 1 month ago) |
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Quote:
SpitballJedi said:
Quote:
ArmFromTheAbyss said: It may take several transfers to see rhizomorphs.
Mycelium can be cottony on an agar dish only to become rhizomorphic when transferred to grain. Colonized grain can be cottony only to become rhizomorphic when spawned to a bulk sub. IMO, selecting random pieces of rhizomorphic mycelium is just as much of a crap-shoot as MS. The presence of a rhizomorph on a petri dish is a not a direct indication of a good fruiting or potent strain. I open my dishes in front of a flow hood to air them out and induce pinning. Then I select a good pin and clone it. Typically only 1 or 2 more transfers will give a monoculture of a fruiting strain.
The way RR does it is like taking an apple and planting all of its seeds, then waiting for them to grow into trees and seeing if they happened to produce good fruit. If you've got the time and it makes you happy then do it that way.
You just blew my mind
Couldn't have said it better considering I'm trying to figure out which of my 18 isolates is the best....
Arm, do you just take the top of the petri dish off and that's it? Won't there be too much FAE and it'll dry out too fast?
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HeadFood
Shroomer



Registered: 12/30/07
Posts: 390
Last seen: 11 years, 1 month
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: HeadFood]
#17416236 - 12/19/12 09:06 AM (11 years, 1 month ago) |
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Can anyone explain in detail this method that ArmFromTheAbyss is talking about?
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Jcoonan
Stranger


Registered: 06/14/12
Posts: 170
Last seen: 10 years, 6 months
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: HeadFood]
#17416311 - 12/19/12 09:30 AM (11 years, 1 month ago) |
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What he is saying is he fruits his agar then selects a clone from what he presumes is a monoculture and clones it to ensure the phenotype he selected actually fruits.
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  Disclaimer: All posts by this user are fictious and outright forgeries of posts and pictures found elsewhere on the Internet. Do not heed any advice or suggestions as they are baseless and without merit. All posts are for entertainment and entertainment alone and should not be confused for fact or truth.
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skippydude
Myco-curious



Registered: 09/08/12
Posts: 1,827
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: Jcoonan]
#17416334 - 12/19/12 09:39 AM (11 years, 1 month ago) |
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ArmFromTheAbyss
Old Hand

Registered: 10/09/02
Posts: 1,368
Loc: Down here in Babylon
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: skippydude]
#17416624 - 12/19/12 11:03 AM (11 years, 1 month ago) |
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Quote:
HeadFood said: Can anyone explain in detail this method that ArmFromTheAbyss is talking about?
This first happened to me by accident. I made a "no pour" agar jar using only potato broth and agar. The lid had gas exchange. I forgot about it and after about 2 weeks checked on it to see a little pin cluster.
When I opened the lid in front of my flow hood to clone the biggest pin it induced more pinning. A few days later the entire thing was pinning. After transferring the clone it only took 1 more transfer to get a monoculture that was vigorous and rhizomorphic.
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HeadFood
Shroomer



Registered: 12/30/07
Posts: 390
Last seen: 11 years, 1 month
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: ArmFromTheAbyss]
#17417593 - 12/19/12 03:17 PM (11 years, 1 month ago) |
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Quote:
Jcoonan said: What he is saying is he fruits his agar then selects a clone from what he presumes is a monoculture and clones it to ensure the phenotype he selected actually fruits.
Thanks for the reply but not what I was asking. Pretty sure that part was self explanatory.
Quote:
ArmFromTheAbyss said:
Quote:
HeadFood said: Can anyone explain in detail this method that ArmFromTheAbyss is talking about?
This first happened to me by accident. I made a "no pour" agar jar using only potato broth and agar. The lid had gas exchange. I forgot about it and after about 2 weeks checked on it to see a little pin cluster.
When I opened the lid in front of my flow hood to clone the biggest pin it induced more pinning. A few days later the entire thing was pinning. After transferring the clone it only took 1 more transfer to get a monoculture that was vigorous and rhizomorphic.
Oh ok so you only had the petri open for a quick second and that induced the pinning. I think I will try this with my plates. Do you think this will also work to find a p.tampanesis or p.galindoi that will fruit?
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