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Offlinecarnage
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Registered: 02/25/03
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Last seen: 7 years, 5 months
test tubes for agar
    #1741453 - 07/23/03 04:01 PM (13 years, 8 months ago)

In all the agar teks I have read, it says to use test tubes with screw caps. How important is this? I have a ton of them with stoppers, and I would hate to think I cannot use them, but I don't want to risk messing up my cultures. Would the rubber stoppers be just as well to use?


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OfflineSuntzu
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Re: test tubes for agar [Re: carnage]
    #1742042 - 07/23/03 07:44 PM (13 years, 8 months ago)

Quite likely they will work fine. In the places I've worked before the screw caps were much preferred because you could inoculate and leave the caps unscrewed just a little bit for gas exchange. There is a decent amount of airspace inside most agar slants, quite sufficient I'd imagine to get decent growth with a rubber stopper.
You may want to think about other simple methods for capping such tubes. A small square of aluminum foil folded over top will mold [no pun] into quite a nice little cover that does provide air exchange, easy to slip on and off. Many other possibilities.
Good luck


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Offlinecarnage
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Registered: 02/25/03
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Last seen: 7 years, 5 months
Re: test tubes for agar [Re: Suntzu]
    #1742115 - 07/23/03 08:05 PM (13 years, 8 months ago)

Thanks for your input!


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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