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TheUnknownPoet
Stranger



Registered: 11/14/12
Posts: 349
Last seen: 8 years, 11 months
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Ergot cultivation
#17414130 - 12/18/12 08:04 PM (11 years, 4 months ago) |
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I plan on trying to cultivate ergot, and yes I know what it can do to you. I am well aware of the risks involved.
In fact, i'm so paranoid, I am building completely sealed positive pressure glovebox out of 1/2 inch plexiglass, and doing all the work in there, never once renmoving the ergot culture.
Of I use silocn to seal the inside corners of the box, nothing will escape, right? I should be fine...
(i'm asking because i don't know if standard silicon would work in such an application...)`
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BlackPeace
Self proclaimed CEO



Registered: 11/18/07
Posts: 1,069
Loc: Canada
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Positive pressure?
I guess you would eant the complete opposite... But then it would render the glovebox almost useless.
Why grow ergot?
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teal
Art of Troll

Registered: 04/14/11
Posts: 137
Last seen: 11 years, 28 days
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That's overkill. Ergot won't do anything to you unless you consume it. Even then, it needs a certain environment to produce the alkaloids of concern. Even if you let the spores out in your house, there's no suitable medium for it to grow on to pose a health hazard.
Having grown C. paspali, I can tell you there is almost no risk involved at all (and C. paspali is a more interesting fungi, you should buy it instead). Once you isolate the right strain and produce the right environment for alkaloid production, then you can worry about the risks (which involves avoiding ingesting or skin contact with the alkaloid enriched solution).
It's not actively growing ergot that's poisonous, it's an overdose of the actives. And getting it to produce actives in the lab is no small feat!
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TheUnknownPoet
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Re: Ergot cultivation [Re: teal]
#17414314 - 12/18/12 08:35 PM (11 years, 4 months ago) |
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Quote:
teal said: That's overkill. Ergot won't do anything to you unless you consume it. Even then, it needs a certain environment to produce the alkaloids of concern. Even if you let the spores out in your house, there's no suitable medium for it to grow on to pose a health hazard.
Having grown C. paspali, I can tell you there is almost no risk involved at all (and C. paspali is a more interesting fungi, you should buy it instead). Once you isolate the right strain and produce the right environment for alkaloid production, then you can worry about the risks (which involves avoiding ingesting or skin contact with the alkaloid enriched solution).
It's not actively growing ergot that's poisonous, it's an overdose of the actives. And getting it to produce actives in the lab is no small feat!
No, i need ergot for this... I know it's a bit overkill, but i need that glovebocks anyhow, i thought this would be a perfect excuse to build one...
Quote:
BlackPeace said:
Positive pressure?
I guess you would eant the complete opposite... But then it would render the glovebox almost useless.
Why grow ergot?
i'd like to grow ergot because I want to give myself gangrene. No, that was sarcasm. Why do you think i'd want ergot? it's painfully obvious, i'd say. -.-
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teal
Art of Troll

Registered: 04/14/11
Posts: 137
Last seen: 11 years, 28 days
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C. paspali can produce alkaloids in a liquid culture (it needs to be the right strain though). Ergot I believe cannot[citation needed].
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ps118
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Registered: 10/05/12
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Re: Ergot cultivation [Re: teal]
#17414426 - 12/18/12 08:59 PM (11 years, 4 months ago) |
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Quote:
completely sealed positive pressure glovebox out of 1/2 inch plexiglass
Cannot be completely sealed AND positive pressure. If you plan on pressurizing it for YOUR protection, you'd need a filter on the outlet. If you want to pressurize it for your CULTURES protection, you need a filter on the inlet (like a laminar flow hood.)
Note: I don't really recommend pressurizing a glovebox. Nothing wrong with lysol.
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TheUnknownPoet
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Re: Ergot cultivation [Re: ps118]
#17415425 - 12/19/12 12:58 AM (11 years, 4 months ago) |
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Quote:
ps118 said:
Quote:
completely sealed positive pressure glovebox out of 1/2 inch plexiglass
Cannot be completely sealed AND positive pressure. If you plan on pressurizing it for YOUR protection, you'd need a filter on the outlet. If you want to pressurize it for your CULTURES protection, you need a filter on the inlet (like a laminar flow hood.)
Note: I don't really recommend pressurizing a glovebox. Nothing wrong with lysol.
Alright, just making usre before i start my LSD tek.
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nooneman


Registered: 04/24/09
Posts: 14,700
Loc: Utah
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Quote:
teal said: getting it to produce actives in the lab is no small feat!
This. Sandoz worked a long time to get a strain that would produce a good amount. C. paspali would be a better starting point.
Also, as was already said, ergot is harmless unless you eat it. That's why it's so easy to infect bread with, because you won't notice that anything is wrong until you eat the bread.
Edited by nooneman (12/19/12 01:13 AM)
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eLeSDenes
Mycelium Expander




Registered: 05/25/11
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Re: Ergot cultivation [Re: nooneman]
#17418071 - 12/19/12 05:12 PM (11 years, 4 months ago) |
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i suggest you to read a little bit more before you make lsd or you get yourself killed. you know it is not like pouring this to that.
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TheUnknownPoet
Stranger



Registered: 11/14/12
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Re: Ergot cultivation [Re: eLeSDenes]
#17418121 - 12/19/12 05:23 PM (11 years, 4 months ago) |
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Quote:
eLeSDenes said: i suggest you to read a little bit more before you make lsd or you get yourself killed. you know it is not like pouring this to that.
thank you for your input. I suppose you have a phd in organic chem as well?
I just needed to know if silicone from home depot is good enough to contain something like this. -.-
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teal
Art of Troll

Registered: 04/14/11
Posts: 137
Last seen: 11 years, 28 days
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Quote:
TheUnknownPoet said: Alright, just making usre before i start my LSD tek.
Quote:
TheUnknownPoet said: thank you for your input. I suppose you have a phd in organic chem as well?
May I recommend you start with something easier like an MDMA tek?
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TheUnknownPoet said: I just needed to know if silicone from home depot is good enough to contain something like this. -.-
on second thought... begin with shake n' bake meth 
It may not be obvious to you, but you're not fooling anyone with your chemistry/microbiology experience. You would not be asking these questions if you were not a noob like the rest of us. If you go this route, you will be wasting money on a lot of unnecessary equipment.
If you want to get started in producing ergot for alkaloid production, that's cool. All you need is some agar plates and an ergot culture. There's a lot of literature (ie. very well written teks) on how to produce alkaloids using C. paspali in a liquid culture. You will be shit out of luck using wild C. purpurea.
You can buy C. paspali from various fungal exchange sites around the world for ~$150. It grows easily on PDA and is not toxic and does not produce spores. Here's some I grew 1 year ago on PDA slants; there's nothing to it. Once you get to that point, you can start thinking about submerged liquid cultures.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
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Loc: Seattle
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Re: Ergot cultivation [Re: teal]
#17419121 - 12/19/12 08:39 PM (11 years, 4 months ago) |
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Why not just get some organic rye berries and toss them into a patch in your yard? Ergot will appear naturally if it's in the grains and if you get organic, there's a good chance it will be there. Here's what happens when you sow rye berries. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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goldenroad08
OwsleyWannabee



Registered: 11/11/12
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Re: Ergot cultivation [Re: eLeSDenes]
#17419526 - 12/19/12 10:06 PM (11 years, 4 months ago) |
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Quote:
eLeSDenes said: i suggest you to read a little bit more before you make lsd or you get yourself killed. you know it is not like pouring this to that.
Hes probably more likley to get arrested and 20-40 years before he gets close to completeing the synthesis.
-------------------- Trades
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TheUnknownPoet
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Registered: 11/14/12
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Last seen: 8 years, 11 months
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Quote:
RogerRabbit said: Why not just get some organic rye berries and toss them into a patch in your yard? Ergot will appear naturally if it's in the grains and if you get organic, there's a good chance it will be there. Here's what happens when you sow rye berries. RR

... do you live inside of a hill?
I am so jelous right now....
I actually have spores, i've just never cultivated this before and I'm scared as tits to try, that's all.
While i may not be able to Synth LSD, (I'm aware it's not the easiest thing in the world) That's not to say I won't learn anything from doing things.
Yea, I could read the forums on how hard it is, and personally, i've put up a few LSD teks, (which were not my own) that I know works.
Looking at the procedure, it isn't really THAT difficult. The reagents are difficult to acquire, but the reaction itself is not OMGTHISISCOMPLEXYOWILLKILLYOURSELF, so long as you're not an idiot. (Ohh, i wonder what this chemical tastes like)
Meth is too easy btw, it's a simple psuedoephedrine reduction. (theoretically, not that i've ever tried and if i have, i dont condone the use of meth, so i probably threw it all away. theoretically.)
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knarkkorven
Entheoholic


Registered: 06/22/05
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Loc: Sweden
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If you don't live in a desert, you should be able to find ergot on several grass species during the end of summer. If you cultivate these, they will produce stroma which can be used in a solution to colonize more grass, creating a big enough harvest for further extractions... Probably better than growing on agar.
http://www.shroomery.org/forums/showflat.php/Number/16709127 http://www.shroomery.org/forums/showflat.php/Number/13180405 http://www.shroomery.org/forums/showflat.php/Number/14762126
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