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InvisibleZarotti
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Registered: 05/12/12
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Can you always achieve Rhyzomorphic Myzelium when using Agar ?
    #17412882 - 12/18/12 06:43 PM (9 years, 1 month ago)

Couple weeks ago dripped a few drops of a Spore Syringe on agar

->The No pour agar jar (No pour Agar Tek) showed multiple colonies with (only) cotton myzelium -no rhyzomorphic growth (as expected)

->Did the first transfer -6days old so far only thin/fuzzy/cotton myzelium visible


Q:---> If you keep on transfering the thickest,leading edge of your cotton agar myzelium will there be a time where rhyzomorpic growth appears ?

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Just read this Thread about moisture in relevance to rhyzomorphic growth

http://www.shroomery.org/forums/showflat.php/Number/10561634#10561634

^ iS this representable ?

If your agar is wet -->fuzzy myzelium
If its dry          -->Rhyzomorphic thick myzelium ?


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Offlinetwistedty
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: Zarotti]
    #17412896 - 12/18/12 06:45 PM (9 years, 1 month ago)

use the search engine, RR states somewhere the less nutrients you use in agar the more rhiz you get

i dont know the recipe or nothing though


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InvisibleArmFromTheAbyss
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: Zarotti]
    #17413156 - 12/18/12 07:30 PM (9 years, 1 month ago)

It may take several transfers to see rhizomorphs.

Mycelium can be cottony on an agar dish only to become rhizomorphic when transferred to grain. Colonized grain can be cottony only to become rhizomorphic when spawned to a bulk sub. IMO, selecting random pieces of rhizomorphic mycelium is just as much of a crap-shoot as MS. The presence of a rhizomorph on a petri dish is a not a direct indication of a good fruiting or potent strain. I open my dishes in front of a flow hood to air them out and induce pinning. Then I select a good pin and clone it. Typically only 1 or 2 more transfers will give a monoculture of a fruiting strain.

The way RR does it is like taking an apple and planting all of its seeds, then waiting for them to grow into trees and seeing if they happened to produce good fruit. If you've got the time and it makes you happy then do it that way.


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InvisibleSpitballJedi
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: ArmFromTheAbyss]
    #17413218 - 12/18/12 07:41 PM (9 years, 1 month ago)

Quote:

ArmFromTheAbyss said:
It may take several transfers to see rhizomorphs.

Mycelium can be cottony on an agar dish only to become rhizomorphic when transferred to grain. Colonized grain can be cottony only to become rhizomorphic when spawned to a bulk sub. IMO, selecting random pieces of rhizomorphic mycelium is just as much of a crap-shoot as MS. The presence of a rhizomorph on a petri dish is a not a direct indication of a good fruiting or potent strain. I open my dishes in front of a flow hood to air them out and induce pinning. Then I select a good pin and clone it. Typically only 1 or 2 more transfers will give a monoculture of a fruiting strain.

The way RR does it is like taking an apple and planting all of its seeds, then waiting for them to grow into trees and seeing if they happened to produce good fruit. If you've got the time and it makes you happy then do it that way.




You just blew my mind


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InvisibleFrankHorrigan
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: ArmFromTheAbyss]
    #17413356 - 12/18/12 08:02 PM (9 years, 1 month ago)

Quote:

ArmFromTheAbyss said:
It may take several transfers to see rhizomorphs.

Mycelium can be cottony on an agar dish only to become rhizomorphic when transferred to grain. Colonized grain can be cottony only to become rhizomorphic when spawned to a bulk sub. IMO, selecting random pieces of rhizomorphic mycelium is just as much of a crap-shoot as MS. The presence of a rhizomorph on a petri dish is a not a direct indication of a good fruiting or potent strain.




:whathesaid:
Couldn't have said it better myself.
I prefer planting all the seeds though :lol:


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Here is how I get things done.
You should take a look. :hehehe:


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OfflineHeadFood
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: SpitballJedi]
    #17413462 - 12/18/12 08:13 PM (9 years, 1 month ago)

Quote:

SpitballJedi said:
Quote:

ArmFromTheAbyss said:
It may take several transfers to see rhizomorphs.

Mycelium can be cottony on an agar dish only to become rhizomorphic when transferred to grain. Colonized grain can be cottony only to become rhizomorphic when spawned to a bulk sub. IMO, selecting random pieces of rhizomorphic mycelium is just as much of a crap-shoot as MS. The presence of a rhizomorph on a petri dish is a not a direct indication of a good fruiting or potent strain. I open my dishes in front of a flow hood to air them out and induce pinning. Then I select a good pin and clone it. Typically only 1 or 2 more transfers will give a monoculture of a fruiting strain.

The way RR does it is like taking an apple and planting all of its seeds, then waiting for them to grow into trees and seeing if they happened to produce good fruit. If you've got the time and it makes you happy then do it that way.




You just blew my mind





Couldn't have said it better considering I'm trying to figure out which of my 18 isolates is the best....

Arm, do you just take the top of the petri dish off and that's it?  Won't there be too much FAE and it'll dry out too fast?


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OfflineHeadFood
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: HeadFood]
    #17416236 - 12/19/12 11:06 AM (9 years, 1 month ago)

Can anyone explain in detail this method that ArmFromTheAbyss is talking about?


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OfflineJcoonan
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Registered: 06/14/12
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: HeadFood]
    #17416311 - 12/19/12 11:30 AM (9 years, 1 month ago)

What he is saying is he fruits his agar then selects a clone from what he presumes is a monoculture and clones it to ensure the phenotype he selected actually fruits.


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Offlineskippydude
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: Jcoonan]
    #17416334 - 12/19/12 11:39 AM (9 years, 1 month ago)

:takingnotes:


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InvisibleArmFromTheAbyss
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Registered: 10/09/02
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: skippydude]
    #17416624 - 12/19/12 01:03 PM (9 years, 1 month ago)

Quote:

HeadFood said:
Can anyone explain in detail this method that ArmFromTheAbyss is talking about?




This first happened to me by accident. I made a "no pour" agar jar using only potato broth and agar. The lid had gas exchange. I forgot about it and after about 2 weeks checked on it to see a little pin cluster.

When I opened the lid in front of my flow hood  to clone the biggest pin it induced more pinning. A few days later the entire thing was pinning. After transferring the clone it only took 1 more transfer to get a monoculture that was vigorous and rhizomorphic.


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OfflineHeadFood
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Re: Can you always achieve Rhyzomorphic Myzelium when using Agar ? [Re: ArmFromTheAbyss]
    #17417593 - 12/19/12 05:17 PM (9 years, 1 month ago)

Quote:

Jcoonan said:
What he is saying is he fruits his agar then selects a clone from what he presumes is a monoculture and clones it to ensure the phenotype he selected actually fruits.





Thanks for the reply but not what I was asking. Pretty sure that part was self explanatory.


Quote:

ArmFromTheAbyss said:
Quote:

HeadFood said:
Can anyone explain in detail this method that ArmFromTheAbyss is talking about?




This first happened to me by accident. I made a "no pour" agar jar using only potato broth and agar. The lid had gas exchange. I forgot about it and after about 2 weeks checked on it to see a little pin cluster.

When I opened the lid in front of my flow hood  to clone the biggest pin it induced more pinning. A few days later the entire thing was pinning. After transferring the clone it only took 1 more transfer to get a monoculture that was vigorous and rhizomorphic.





Oh ok so you only had the petri open for a quick second and that induced the pinning.  I think I will try this with my plates.  Do you think this will also work to find a p.tampanesis or p.galindoi that will fruit?


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