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OfflineeLeSDenes
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How many petris to get a pure isolate
    #17411117 - 12/18/12 08:51 AM (11 years, 4 months ago)

Hello,
I was just thinking and tried to do the math that how many petri plates i have to use to get to uniformly growing pure strains if i start from MC. Let's say i make 10 plates  to start with. Each of them will have good looking sectors so let's assume i have 4-5 good looking sectors from each plate. Until i get down to single sectors is probably more than 7 transfers . That must be a lot of plates. But from the original spore swipe there are more good looking sectors and each sectors have a few strains. If i isolate all of them how many plates i have to use. I assume 100 plates is not enough:D
Sorry if i am hard to follow. please help me with the math if you have done it before. Thank you for any answer in advance.

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OfflineWorkmanV
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Re: How many petris to get a pure isolate [Re: eLeSDenes] * 1
    #17411885 - 12/18/12 12:33 PM (11 years, 4 months ago)

How about cloning the best (depending on your criteria) mushroom from a multispore cultivation.  I prefer to select based on the end result instead of guessing the best mycelium to choose from a petri dish.  You may have to do a bit more sector selection after cloning since an individual mushroom can be composed of more than one strain of mycelium.  This method uses far fewer petri dishes and time.


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OfflineeLeSDenes
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Re: How many petris to get a pure isolate [Re: Workman]
    #17412393 - 12/18/12 02:36 PM (11 years, 4 months ago)

You may be right. I was thinking about this but when i was searching on the forum RR advised  to start with spores because that way you can isolate more strains. I also heard that an isolation from a clone can be weaker than the composition of the strains. But if it works out for you then i will give it a try as well! Thank you!

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OfflineTheUnknownPoet
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Re: How many petris to get a pure isolate [Re: eLeSDenes]
    #17414140 - 12/18/12 08:06 PM (11 years, 4 months ago)

Isn;t this called a monoculture?

Why would you want to do this? Are there any benefits?

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OfflineeLeSDenes
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Re: How many petris to get a pure isolate [Re: TheUnknownPoet]
    #17418001 - 12/19/12 04:57 PM (11 years, 4 months ago)

no there is no benefit, I just like to waste my time lol:D

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Invisiblemycoelf
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Re: How many petris to get a pure isolate [Re: eLeSDenes]
    #17418010 - 12/19/12 04:59 PM (11 years, 4 months ago)

usually less then 6 generations and a total of less then 20 plates. Isolates are not so hard if you start you MSG with the proper dilution of spores.


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OfflineeLeSDenes
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Re: How many petris to get a pure isolate [Re: eLeSDenes]
    #17418037 - 12/19/12 05:06 PM (11 years, 4 months ago)

let's see:D i'll post the pictures and the number of plates i used up for that:D

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OfflineBjJiggles
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Re: How many petris to get a pure isolate [Re: eLeSDenes]
    #17420019 - 12/19/12 11:55 PM (11 years, 4 months ago)

I agree with workman, cloning from a nice fruit is the way to go IMO... You have far less strains to sort through for your Isolate and you know the strains are capable of fruiting and have the genetics you seek.:2cents:


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Invisible36fuckin5
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Re: How many petris to get a pure isolate [Re: Workman]
    #17425468 - 12/21/12 12:04 AM (11 years, 4 months ago)

Quote:

Workman said:
How about cloning the best (depending on your criteria) mushroom from a multispore cultivation.  I prefer to select based on the end result instead of guessing the best mycelium to choose from a petri dish.  You may have to do a bit more sector selection after cloning since an individual mushroom can be composed of more than one strain of mycelium.  This method uses far fewer petri dishes and time.




This is exactly how I do it. It saves a lot of time and materials compared to having to fruit out several dozen monocultures that may or may not produce.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Re: How many petris to get a pure isolate [Re: 36fuckin5]
    #17436438 - 12/23/12 10:42 AM (11 years, 4 months ago)

Quote:

36fuckin5 said:
Quote:

Workman said:
How about cloning the best (depending on your criteria) mushroom from a multispore cultivation.  I prefer to select based on the end result instead of guessing the best mycelium to choose from a petri dish.  You may have to do a bit more sector selection after cloning since an individual mushroom can be composed of more than one strain of mycelium.  This method uses far fewer petri dishes and time.




This is exactly how I do it. It saves a lot of time and materials compared to having to fruit out several dozen monocultures that may or may not produce.




:thumbup:

I'm going to go ahead and assume you're growing P. cubensis...
Do MS monotubs/trays (bulk) for the best variety of choices and clone what you're looking for- clusters, largest fruit, fattest fruit, etc.

I've been following a friend of mine who has cloned PE fruits over and over again a few times, and he recently monocultured an isolate that he originally isolated 8+ months ago... he claims that he was trying to come up with short, fat, and hella thick penises... he nicknamed his creation P. Cubensis "fat chode" and they were just that.

He also claimed that shorter, fatter, and thicker PEs would be more "potent"... now idk much about cube cultivation and idk if that is scientifically accurate, but nonetheless he stands behind his work with a crooked "ive been tripping balls for months" smile on his face. None were taller than 5 or 6 inches; however, the vast majority weighed in at or around 100 grams wet. Many were wider than they were tall- no joke. It was a strange but wicked sight to see.

So, as you can tell from my little rant, isolating is fun and the results are well worth the time and effort- you learn a lot too! good luck becoming a mad scientist.. just don't literally become mad like my friend and rant on all day about your "fat chodes".

cheers from :sunny: FL

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Invisible36fuckin5
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Re: How many petris to get a pure isolate [Re: Mykes logos]
    #17448658 - 12/25/12 10:32 PM (11 years, 4 months ago)

Quote:

Mykes logos said:
Quote:

36fuckin5 said:
Quote:

Workman said:
How about cloning the best (depending on your criteria) mushroom from a multispore cultivation.  I prefer to select based on the end result instead of guessing the best mycelium to choose from a petri dish.  You may have to do a bit more sector selection after cloning since an individual mushroom can be composed of more than one strain of mycelium.  This method uses far fewer petri dishes and time.




This is exactly how I do it. It saves a lot of time and materials compared to having to fruit out several dozen monocultures that may or may not produce.




:thumbup:

I'm going to go ahead and assume you're growing P. cubensis...




Species doesn't matter. It pretty much works with any species. Some species won't fruit from MS, however, and in that case you'd do best to collect and clone a wild specimen.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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