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2bafungi2
Rhizomorph



Registered: 11/25/12
Posts: 41
Loc: Canada
Last seen: 11 years, 1 month
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Brf cake -inoculate 10cc liquid mycelium?
#17378316 - 12/12/12 03:50 AM (11 years, 5 months ago) |
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Greetings, This is my first post. Has anyone had success with hitting a cake using extra LC? Buddy has an abundance of malt/dextrose LC. Looks very healthy.. strands,not clumping. He wants to speed colonization by adding more volume of the lc. Is it possible to make a PF cake come out dependably sterile using a "dry" mixture. He thought that by using 8cc less water in the preparation will allow for the extra moisture from the 10cc of LC. Can this strategy be effectively applied to rye spawn? Sorry if this is a tedious question. I found no clear answer in the forums yet.
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indahighcountry
Stranger

Registered: 08/03/12
Posts: 49
Last seen: 10 years, 9 months
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Re: Brf cake -inoculate 10cc liquid mycelium? [Re: 2bafungi2]
#17385013 - 12/13/12 10:40 AM (11 years, 5 months ago) |
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2 things
1) this is pretty basic stuff -- expect a mod to move it to the mushroom cultivation section
2) more LC will help to a point -- don't want too much liquid in the cake less it get anaerobic at points and stall out or get moldy, however 3-5cc per 1/2 pint shouldn't be too much trouble, just remember to mix them up after injecting -- don't want to disrupt your verm layer on top but do want the liquid to spread
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2bafungi2
Rhizomorph



Registered: 11/25/12
Posts: 41
Loc: Canada
Last seen: 11 years, 1 month
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Re: Brf cake -inoculate 10cc liquid mycelium? [Re: indahighcountry]
#17388502 - 12/13/12 09:31 PM (11 years, 5 months ago) |
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Thanks Dude, I am going to suggest he try a few experiments with reducing H2O in 5ml increments over 6 jars of rye. Then knock with equal volumes of thick suspended myc. Shake well and incubate. It is probably going to send him back to standard recipe. It is worth a try and I will likely start a grow log to present the results.
-------------------- Red Spot
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2bafungi2
Rhizomorph



Registered: 11/25/12
Posts: 41
Loc: Canada
Last seen: 11 years, 1 month
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Re: Brf cake -inoculate 10cc liquid mycelium? [Re: 2bafungi2]
#17388892 - 12/13/12 10:29 PM (11 years, 5 months ago) |
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Perhaps he can produce a PF "style" series of jars, but use pasteurized (then baked to dry) coir to replace 30% to 90% of the brf. This should reduce spawn density, and "soup-iness" that cause inherent anaerobic zones when applying more LC. I realize that the pasteurize/bake may be overkill. Any thoughts or direction is highly appreciated...
I am a newb and probably started this topic in the wrong place. My apologies
-------------------- Red Spot
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2bafungi2
Rhizomorph



Registered: 11/25/12
Posts: 41
Loc: Canada
Last seen: 11 years, 1 month
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Re: Brf cake -inoculate 10cc liquid mycelium? [Re: 2bafungi2]
#17388998 - 12/13/12 10:49 PM (11 years, 5 months ago) |
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We do understand.... Higher spawn density is important in order to achieve a more robust and productive bulk substrate. He just wants to expend a surplus volume of liquid myc before it ages, and try to find a faster road to fruit. Storage capacity is maxed. Waste not want not.
-------------------- Red Spot
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2bafungi2
Rhizomorph



Registered: 11/25/12
Posts: 41
Loc: Canada
Last seen: 11 years, 1 month
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Re: Brf cake -inoculate 10cc liquid mycelium? [Re: 2bafungi2]
#17389240 - 12/13/12 11:55 PM (11 years, 5 months ago) |
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Light Bulb! What if my bud placed sterilized spawn jars in an oven, (loose lids) and baked off some moisture? A scale would come in handy in order to accurately conduct and document the experiment. Weigh the wet jars and rotate a series of 6 jars through a pre heated oven at incremental intervals. (5 min - 25 min) Record resulting weight and knock each with same volume of liquid myc. This may provide a benchmark for the max vol of LC to be effectively added to a specific spawn after "dehydration" @ "x" minutes.
-------------------- Red Spot
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2bafungi2
Rhizomorph



Registered: 11/25/12
Posts: 41
Loc: Canada
Last seen: 11 years, 1 month
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Re: Brf cake -inoculate 10cc liquid mycelium? [Re: 2bafungi2]
#17389370 - 12/14/12 12:37 AM (11 years, 5 months ago) |
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A side topic: Bud was thinking of trying to produce "freezer Bags" of pasteurized coir to knock with extra myc solution. Prepare coir on the dry side and carefully spoon into freezer storage bags. Use a polyfill plug secured by elastics at bag opening. Apply micro pore tape on several alcohol swabbed spots for multi inoculation points. Hit It Hard with extra myc solution. Squeeze to Distribute and Compress, then incubate. He is going to try this, as he expects the contaminate resistance of the coir to allow healthy colonization.
-------------------- Red Spot
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2bafungi2
Rhizomorph



Registered: 11/25/12
Posts: 41
Loc: Canada
Last seen: 11 years, 1 month
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Re: Brf cake -inoculate 10cc liquid mycelium? [Re: 2bafungi2]
#17389716 - 12/14/12 02:47 AM (11 years, 5 months ago) |
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How about a bag of pasteurized PF tek substrate?
Maybe mix a "dry" batch and load into pillow case and pasteurize. One would bake the brf and verm first, to reduce contams. This might provide a viable substrate for heavy myc solution inoculation.
-------------------- Red Spot
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
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Re: Brf cake -inoculate 10cc liquid mycelium? [Re: 2bafungi2]
#17408773 - 12/14/12 02:47 AM (11 years, 5 months ago) |
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This thread has been closed.
Reason: Not an advanced mycology topic.
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