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OfflineBasicMyco
Stranger
Registered: 11/30/12
Posts: 10
Last seen: 10 years, 28 days
No fruits after 1 month : Need advice before starting over
    #17307701 - 11/30/12 08:07 AM (12 years, 1 month ago)

To start off, thanks for all the great information. I've been reading this website every day the past few months, and the past month I've been reading exponentially more to try and troubleshoot my SGFCs.

I had 5 cambodian jars make it to drunk/roll/birth, and they were birthed a month ago after a 1 week consolidation. My 6 treasure coast jars took longer and were birthed 1 week ago, with consolidation/dunk/roll/birth. I'll describe the failed process of the cambodian jars so I don't make the same mistake for the TCs. After a month, I have a few aborts but nothing to show. The cambodians are now turning yellow all over and smell like urine. The mycelium didn't even overgrow the vermiculite roll, it seems to just pin where there wasn't any verm.

The SGFCs have 4" of perlite, and have 2" separated holes on all 6 sides. After 2 weeks I bought a room humidifier. That didn't help so I stopped using it after a week. After 3 weeks I bought both SGFCs a 6500k lamp. It didn't show any change in 3 days, so I taped closed half of the holes to raise RH. That made a bunch of 1-5mm fruits overnight, but they all seemed to abort. 4 days later they're the same size.

I mist 3-4 times a day, and fan about 6. They used to get indirect sunlight (less light than in the first picture). Once I started using lamps I blocked off a lot more of the natural sunlight, as you'll see in the lamp picture. I've tried days of misting where I spray them indirectly (letting it fall) but it never gets damp enough, so for a few days after I usually spray the cakes directly from 1-1.5 feet away. I've never noticed bruising.

I tried not misting for 2 days to see if I was over-misting (no signs of bluing though) and I got a 6mm abort on the top of a cake. I wonder if maybe my spray bottle doesn't provide a fine enough mist, or if I'm regularly drowing the cakes. I try to get them to feel damp, but not soggy.

On one cake I even tried the voodoo tek, with no response. Temp is always 69-72*F.


More than "indirect" sunlight, for the photos.

Week old TCs.

TC Close up.

Cambos. Bottom middle you can see the dark abort on top. That was from the 2 day hiatus from misting. They got 12/12 light from lamps though.

That abort was from the 2 day hiatus from misting. They got 12/12 light from lamps though.

New primadora from the RH increase. Hasn't grown since it popped up. You can see yellowing on the top of the cake.

More babys from the RH increase of taping some holes shut.

Lighting shot.

Sprayer. Too industrial? I use about 1/2 of it a day between the two SGFCs.

As you can see I'm not too far off overall, I've done a lot of reading, but I'm obviously making one or two CRITICAL mistakes. They should eventually fruit no matter what, and they aren't. Too much water? No idea. Any advice helps, I'm throwing the cambos out now. They smell.

Edited by BasicMyco (11/30/12 08:30 AM)

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InvisibleThadeous
On the path

Registered: 08/02/11
Posts: 1,101
Re: No fruits after 1 month : Need advice before starting over [Re: BasicMyco]
    #17307837 - 11/30/12 08:53 AM (12 years, 1 month ago)

I'll take a stab.

Your stuff looks pretty solid, friend.  I would get a cheap analog hydrometer to ensure your RH is where it needs to be.  If you live in an excessively dry area it can be a bit more difficult to keep it up; conversely if it is very humid, you might be able to fan more or something; either way, it is a good number to know.

If I had to put money down on why these pins didn't fruit it would be genetics.  It doesn't look like your methods are off, but the particular genetics you had didn't vibe with them.  I would't change much, save being more consistent with fanning/misting, and getting that hydrometer. 

With MS (multispore) it is pretty much a crapshoot.  From the same syringe I have had some jars do awesome, and some do awful, even at fruiting stages some tubs have done well, while others didn't do anything. 

:goodluck:

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Offlinedivvy123
STRANGER DANGER

Registered: 06/24/12
Posts: 322
Last seen: 6 years, 13 days
Re: No fruits after 1 month : Need advice before starting over [Re: BasicMyco]
    #17307896 - 11/30/12 09:18 AM (12 years, 1 month ago)

i had a heavy misting bottle and my mycelium yellowed too, then i got a real fine mister from walmart in the hair aisle. that seemed to fix it, i think the spray was hitting the cake too hard.
just retry and hope for the best man! :cheers:

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OfflineBasicMyco
Stranger
Registered: 11/30/12
Posts: 10
Last seen: 10 years, 28 days
Re: No fruits after 1 month : Need advice before starting over [Re: divvy123]
    #17308060 - 11/30/12 10:07 AM (12 years, 1 month ago)

Quote:

Thadeous said:
I'll take a stab.

Your stuff looks pretty solid, friend.  I would get a cheap analog hydrometer to ensure your RH is where it needs to be.  If you live in an excessively dry area it can be a bit more difficult to keep it up; conversely if it is very humid, you might be able to fan more or something; either way, it is a good number to know.

If I had to put money down on why these pins didn't fruit it would be genetics.  It doesn't look like your methods are off, but the particular genetics you had didn't vibe with them.  I would't change much, save being more consistent with fanning/misting, and getting that hydrometer. 

With MS (multispore) it is pretty much a crapshoot.  From the same syringe I have had some jars do awesome, and some do awful, even at fruiting stages some tubs have done well, while others didn't do anything. 

:goodluck:



While I see what you're saying about a single syringe just being bunk, do you think the Treasure Coast batch should have shown signs of growth? They're obviously from a different syringe, from a sponsor. Neither set of cakes really overgrew the vermiculite at all, and the TCs aren't even pinning yet... I appreciate the encouragement though, it's good to know I'm not overlooking something huge.

Quote:

divvy123 said:
i had a heavy misting bottle and my mycelium yellowed too, then i got a real fine mister from walmart in the hair aisle. that seemed to fix it, i think the spray was hitting the cake too hard.
just retry and hope for the best man! :cheers:



For what it's worth I'll try a fine mister. I've seen the one I have used by others on here, but who knows...

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InvisibleThadeous
On the path

Registered: 08/02/11
Posts: 1,101
Re: No fruits after 1 month : Need advice before starting over [Re: BasicMyco]
    #17321164 - 12/02/12 02:41 PM (12 years, 1 month ago)

Here is what the genetics of the particular myc are telling you:  We don't entirely like your conditions for fruiting (or for myc growth in the case of the treasure coast, though, that could be substrate moisture related)

Now, even though you are doing most things right (save not knowing the RH)  they just don't like your setup.  This is why isolates are so nice, because you do tons of grows, and eventually find a single strain of genetics that absolutely love the environment you provide.  It picks you more than you pick it.  MS is absolutely unpredictable despite stable growing conditions.

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OfflineBasicMyco
Stranger
Registered: 11/30/12
Posts: 10
Last seen: 10 years, 28 days
Re: No fruits after 1 month : Need advice before starting over [Re: Thadeous]
    #17339129 - 12/05/12 03:26 PM (12 years, 1 month ago)

After 5 days of fine misting, the new baby fruits haven't grown a single millimeter. They seemed to rot a bit. I plucked them, dunked everything, and now I'm going to rebirth them ALL into a single SGFC in hopes of a higher RH. I removed the tape covering the holes, I think it limited the FAE too much.

Thanks Thadeous, that makes sense. Now if only I can get a single fruit to isolate! :o

I'm going for a "retry" since they'll all be rehydrated, have a normal SGFC, and have a fine mist. If this fails should I try a different spore vendor, or the "lazy invitro poo bags"?

Edited by BasicMyco (12/05/12 03:27 PM)

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OfflineSpecops2594
Shroomer
I'm a teapot User Gallery


Registered: 10/02/12
Posts: 458
Loc: PNW, Oregon Flag
Last seen: 4 years, 7 months
Re: No fruits after 1 month : Need advice before starting over [Re: BasicMyco]
    #17339408 - 12/05/12 04:19 PM (12 years, 1 month ago)

Millions of spores are in a syringe. It only takes 2 germinated spore to form dichariote mycelium. 1 dicharyiotic structure had a completely different set on traits than another with respect to the species. In theory you can get thousands upon thousands of different "strains" in a syringe with different growth habits. Unless the syringe does not contain any viable spores be that age, solution or whatever, that syringe will be no different than any other cube syringe (PE is an exception with stablalized traits).


--------------------

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OfflineRoman08640
Learner


Registered: 03/06/12
Posts: 528
Loc: Earth
Last seen: 7 months, 22 days
Re: No fruits after 1 month : Need advice before starting over [Re: BasicMyco]
    #17339568 - 12/05/12 04:48 PM (12 years, 1 month ago)

Nice setup. I'm a nub but from what I have experienced that's a damn good set up. Sending good grow vibes

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