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Invisiblecozc
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Registered: 06/14/11
Posts: 380
LC in front of flow hood
    #17338066 - 12/05/12 11:49 AM (12 years, 1 month ago)

I don't want to hear about lc's being bad or anything cause ima just do what i do lol.


All of this will be done in front of a tested flowhood.


Is it safe to take the lid off of my lc and noc it with small agar piece?

once its colonized and tested, which of these two methods should I use?

1: open lc lid and suck myc out
2: Leave lid closed and tilt jar and suck myc through injector port?


Thanks in advance!


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OfflineTheCyndicate
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Registered: 10/16/11
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Re: LC in front of flow hood [Re: cozc]
    #17338080 - 12/05/12 11:51 AM (12 years, 1 month ago)

Just use your agar wedge to inoculate a couple grain masters ...... it's faster then a liquid culture .

Cyn

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Invisiblecozc
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Registered: 06/14/11
Posts: 380
Re: LC in front of flow hood [Re: TheCyndicate]
    #17338103 - 12/05/12 11:54 AM (12 years, 1 month ago)

Quote:

TheCyndicate said:
Just use your agar wedge to inoculate a couple grain masters ...... it's faster then a liquid culture .

Cyn





My rye supplier is out for a few weeks and I don't want to 100% waste my nice rhizho's on the leading edge of my plates lol


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OfflineTheCyndicate
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Re: LC in front of flow hood [Re: cozc]
    #17338151 - 12/05/12 12:02 PM (12 years, 1 month ago)

shit try it .
you can always use WBS .
I have done it before with success .
just not anymore . agar>WBS>G2G works alot faster ... IMO

Cyn

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InvisibleTranscendingLife
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Registered: 06/09/10
Posts: 21,627
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Re: LC in front of flow hood [Re: TheCyndicate]
    #17338217 - 12/05/12 12:18 PM (12 years, 1 month ago)

I'm w/ Cyn on this one.
Use an agar wedge to inoculate a master grain jar of WBS.
By the time that's fully colonized, you're guy will probably have rye back in stock.
Also, I prefer to G2G a WBS jar to Rye jars.
More inoculation points.

But, yes, you could do what you described.


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