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InvisibleZarotti
Stranger

Registered: 05/12/12
Posts: 314
Agar doesnt colonize...Can you use a 6 month old pin ?
    #17336506 - 12/05/12 02:54 AM (12 years, 1 month ago)

-Inoculated 4 grain jars 2 weeks ago all show no growth
(The Grains maybe overdosed on gypsum - they are all pretty white)

-5 Days ago inoculated (spore syringe) 5 no pour agar jars ->followed the Tek
no growth not even mold/bacteria  Tempeature : 70 F

Would a 6 month old air dried pin colonize on agar ?

Edited by Zarotti (12/05/12 03:54 AM)

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InvisibleZeet
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Registered: 04/26/12
Posts: 932
Re: Not even Bacteria is growing on my Agar...no growth... [Re: Zarotti] * 1
    #17336515 - 12/05/12 02:58 AM (12 years, 1 month ago)

Yes it would, but you'd have to isolate the fresh mycelium pretty quick as there will be loads of bacteria on that sample(according to RR there would be no bacteria on it? I find it hard to understand how bacteria would not settle onto the sample.. but oh well!).

I have no idea about your first question though, sorry. I tried agar once. Mine colonized, but pretty fucking slowly.

My recipe: Potato flakes, Honey, Agar Agar.

edit: have a read:

Quote:

RogerRabbit said:
Many of the edible culture banks store mycelium in its dry form. The commercial mushroom farms routinely buy dry mycelium to restart on agar.  It helps if there are no imperfect mold spores on the tissue.  Bacteria usually isn't a problem as it is killed by drying.

Perhaps a segment in my upcoming video series can be to take a piece of mycelium from the inside of a fresh mushroom stipe as though I were going to clone it, but instead slip it inside a petri dish with desiccant rather than agar.  I could let the tissue dry for a few months, under sterile conditions, then retrieve and grow it out.  This is how the tissue banks do it.  Dry mycelial tissue will grow again, I assure you.  I've switched over to all legal edibles and medicinals, so I'd have to do it with oyster or something, but you'd get the point.  The procedure would be the same.
RR




Quote:

RogerRabbit said:
With all due respect to those who disagree or have never done it, you can take dried mycelium and get it growing again on agar. I've done this many times with dry mushrooms. The stipe works better than the cap. This is not due to spores germinating, but due to the dried mushroom tissue rehydrating and beginning to grow again.






https://mycotopia.net/forums/cloning/41022-cloning-dried-tissue.html


Edited by Zeet (12/05/12 03:11 AM)

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