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Offlinerichismalls
Richi-smalls


Registered: 10/19/02
Posts: 769
Loc: the ill state
Last seen: 1 year, 2 months
Use of LC lids
    #17333872 - 12/04/12 04:51 PM (12 years, 1 month ago)

how many times can you use a liquid culture lid for a mason jar before the air filter needs to be replaced?  thanks everyone

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InvisiblezMan
zMan

Registered: 09/08/12
Posts: 2,191
Re: Use of LC lids [Re: richismalls]
    #17334022 - 12/04/12 05:13 PM (12 years, 1 month ago)

Long long time.


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Invisibleserotonin storm
Don't be scared, homie!
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Registered: 07/15/12
Posts: 1,178
Loc: Holland
Re: Use of LC lids [Re: zMan]
    #17334213 - 12/04/12 05:42 PM (12 years, 1 month ago)

Depends on the filter material, if you are using post office tyvek then probably every other time if not every time. SFD's and syringe filters will almost last indefinitely


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InvisiblezMan
zMan

Registered: 09/08/12
Posts: 2,191
Re: Use of LC lids [Re: serotonin storm]
    #17334250 - 12/04/12 05:48 PM (12 years, 1 month ago)

Wheps I assumed SFD (synthetic filter discs) were used.


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OfflineNineInchNails
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Registered: 03/01/09
Posts: 1,190
Last seen: 2 years, 12 days
Re: Use of LC lids [Re: zMan]
    #17334407 - 12/04/12 06:21 PM (12 years, 1 month ago)

I've reused tyvek lids many times with no contam. Same with whatman filters.

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OfflineAdrenalien
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Registered: 11/12/12
Posts: 257
Last seen: 8 years, 17 days
Re: Use of LC lids [Re: NineInchNails]
    #17334644 - 12/04/12 07:04 PM (12 years, 1 month ago)

yeah, this totally depends on the type of filter. From the filter materials ive used, Ive had none show signs of breakdown or anything like that even after about PCing them 3 or 4 times.

I will note though, that EZ felt SUCKS as a LC filter material. .... Tyvek and polyfill are better filter materials, IMO. If your willing to spend some big money, go for whatmans. I doubt they are as superior as people bark them up to be, but for the sake of sterility and LCs, they are a small price to pay ($2-3 a piece.. O.o)

And NineInchNails, Ive been following your Simple Tyvek Lids tek (found it through ShroomTalk)... By far my favorite of the simple lid teks, love it! Ive got maybe 40 jars going with it and theyve been going solid! Thanks!

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Offlineps118
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Registered: 10/05/12
Posts: 210
Last seen: 8 years, 10 months
Re: Use of LC lids [Re: Adrenalien]
    #17334775 - 12/04/12 07:23 PM (12 years, 1 month ago)

Quote:

Adrenalien said:
yeah, this totally depends on the type of filter. From the filter materials ive used, Ive had none show signs of breakdown or anything like that even after about PCing them 3 or 4 times.

I will note though, that EZ felt SUCKS as a LC filter material. .... Tyvek and polyfill are better filter materials, IMO. If your willing to spend some big money, go for whatmans. I doubt they are as superior as people bark them up to be, but for the sake of sterility and LCs, they are a small price to pay ($2-3 a piece.. O.o)

And NineInchNails, Ive been following your Simple Tyvek Lids tek (found it through ShroomTalk)... By far my favorite of the simple lid teks, love it! Ive got maybe 40 jars going with it and theyve been going solid! Thanks!




Why do you say easy-felt sucks as a filter material? I haven't had bad luck with it...

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Offlinerichismalls
Richi-smalls


Registered: 10/19/02
Posts: 769
Loc: the ill state
Last seen: 1 year, 2 months
Re: Use of LC lids [Re: richismalls]
    #17334949 - 12/04/12 07:52 PM (12 years, 1 month ago)

this is what im referring to


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Offlinerichismalls
Richi-smalls


Registered: 10/19/02
Posts: 769
Loc: the ill state
Last seen: 1 year, 2 months
Re: Use of LC lids [Re: richismalls]
    #17334950 - 12/04/12 07:52 PM (12 years, 1 month ago)

sorry i wasnt more specific :rolleyes:

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Offlinerichismalls
Richi-smalls


Registered: 10/19/02
Posts: 769
Loc: the ill state
Last seen: 1 year, 2 months
Re: Use of LC lids [Re: richismalls]
    #17334973 - 12/04/12 07:55 PM (12 years, 1 month ago)

ive been doing this for a while and never used them...but i will say they are the sheit!

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OfflineAdrenalien
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Registered: 11/12/12
Posts: 257
Last seen: 8 years, 17 days
Re: Use of LC lids [Re: ps118]
    #17335022 - 12/04/12 08:08 PM (12 years, 1 month ago)

Quote:

ps118 said:
Quote:

Adrenalien said:
yeah, this totally depends on the type of filter. From the filter materials ive used, Ive had none show signs of breakdown or anything like that even after about PCing them 3 or 4 times.

I will note though, that EZ felt SUCKS as a LC filter material. .... Tyvek and polyfill are better filter materials, IMO. If your willing to spend some big money, go for whatmans. I doubt they are as superior as people bark them up to be, but for the sake of sterility and LCs, they are a small price to pay ($2-3 a piece.. O.o)

And NineInchNails, Ive been following your Simple Tyvek Lids tek (found it through ShroomTalk)... By far my favorite of the simple lid teks, love it! Ive got maybe 40 jars going with it and theyve been going solid! Thanks!




Why do you say easy-felt sucks as a filter material? I haven't had bad luck with it...




Its not necessarily that the material is "bad", its just that it is so absorbant that you need to be super careful when swirling the jars. Ive lost far too many LCs from accidentally getting soggy filters :/

If you use a stir plate, it should be fine though I think.

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Invisiblebudmanman
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Registered: 02/07/07
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Re: Use of LC lids [Re: Adrenalien]
    #17335068 - 12/04/12 08:20 PM (12 years, 1 month ago)

I use fish tank tubing and poly fill. Infinite uses.


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Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.

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OfflineAdrenalien
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Re: Use of LC lids [Re: budmanman]
    #17335113 - 12/04/12 08:26 PM (12 years, 1 month ago)

Quote:

budmanman said:
I use fish tank tubing and poly fill. Infinite uses.




Thats a style I really wanted to try, but I had no tubing at the time. Now Im commited to the tyvek :crazy:

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InvisibleWillSolvem
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Re: Use of LC lids [Re: Adrenalien]
    #17335126 - 12/04/12 08:28 PM (12 years, 1 month ago)

I rtv a autoclavable tube to a lid and fill with poly-fil, just bend the tube over before you shake and you can shake jars as hell, never had one get wet or fail, credit the idea to either roadkill or agar, not sure which one gave me the idea.


I fucking love EZ felt for everything else, note jar, lids in background!


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Invisiblebudmanman
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Registered: 02/07/07
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Re: Use of LC lids [Re: WillSolvem]
    #17335157 - 12/04/12 08:35 PM (12 years, 1 month ago)

Well I do not know what autoclave able tubing is but fish tank tubing works fine. I do not bend mine over I just cover them with foil when I pc them.


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.

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InvisibleWillSolvem
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Folding@home Statistics
Registered: 04/24/12
Posts: 1,519
Loc: Chapter 26 Flag
Re: Use of LC lids [Re: budmanman]
    #17335229 - 12/04/12 08:49 PM (12 years, 1 month ago)

Bend them to shake not PC, autoclavable tubing is surgical suction tubes, if your tubing looks "cloudy" and soft after sterilizing its the wrong grade and might leach chemicals into your LC, I grow/sell edibles, if I only did magics I wouldn't care much about it


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AMU Q&A Thread because questions deserve answers.

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