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OfflineAnnoA
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Re: Multi Substrate Test [Re: micro]
    #2167235 - 12/07/03 08:13 PM (17 years, 3 months ago)

How exactly would one use the spectrophotometer without a HPLC?


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Invisiblemicro
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Re: Multi Substrate Test [Re: Anno]
    #2167242 - 12/07/03 08:17 PM (17 years, 3 months ago)

You don't need thaaaat pure of a sample. If the extract has >50% psilocybin you should be able to just dilute it and still get readings.

It's a problem, though, if you have a sample that's not really pure.

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OfflineAnnoA
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Re: Multi Substrate Test [Re: micro]
    #2167258 - 12/07/03 08:25 PM (17 years, 3 months ago)

An acetic acid extraction extracts pretty much all tryptamines (and more) from the mushroom...so that would leave me with an indistinguishable mixture of componenets....
I?m not sure if this would be of any use for the quantitative Psilocybin/Psilocin determination.

Just to see if I understood it right: You would go with the (diluted) extract directly into a spectrophotometer and then read the absorption at 260 nm?


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Invisiblemicro
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Re: Multi Substrate Test [Re: Anno]
    #2167315 - 12/07/03 09:01 PM (17 years, 3 months ago)

I'd have to look up the peaks, but yeah. You'd have a mix of stuff, but if you could get a good mean the results are o.k.

Mushrooms don't produce too many tryptamines, but the extract will have pretty much any amine compound extracted, too. The majority should be psilocybin, though, so if there is any somewhat substantial increase in one specific substrate it should show on the numbers.

I have some doubts that different substrates lead to any substantial increases, though.

Oh, to clarify -- no, I would probably do TLC, or something, but if you are looking for a quick and dirty way to do things, that would be enough, unless we're talking changes < 10% or whatever.

--
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Edited by micro (12/07/03 09:03 PM)


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OfflineExtravagantDream
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Re: Multi Substrate Test [Re: Anno]
    #2171171 - 12/11/03 03:41 AM (17 years, 2 months ago)

Quote:

Anno said:
Only a couple of casings fruited. So I won?t be able to use this batch for much.

I will repeat the experiment on a later time.




which ones did fruit?


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Post deleted by Administrator [Re: Anno]
    #2171177 - 12/11/03 03:49 AM (17 years, 2 months ago)



--------------------
-Alien :cool:

Alien Substrate Instructional:  http://www.youtube.com/watch?v=KG82xLzrNg8


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OfflineBaby_Hitler
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Re: Multi Substrate Test [Re: Alien]
    #2171303 - 12/11/03 05:02 PM (17 years, 2 months ago)

kthbye.


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InvisibleAlien
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Post deleted by Administrator [Re: Baby_Hitler]
    #2171474 - 12/11/03 08:57 PM (17 years, 2 months ago)



--------------------
-Alien :cool:

Alien Substrate Instructional:  http://www.youtube.com/watch?v=KG82xLzrNg8


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Re: Multi Substrate Test [Re: Alien]
    #2171554 - 12/11/03 09:51 PM (17 years, 2 months ago)

kthbye = Okay, thank you, goodbye


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InvisibleAlien
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Post deleted by Administrator [Re: Effed]
    #2171568 - 12/11/03 10:00 PM (17 years, 2 months ago)



--------------------
-Alien :cool:

Alien Substrate Instructional:  http://www.youtube.com/watch?v=KG82xLzrNg8


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OfflineAnnoA
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Re: Multi Substrate Test [Re: Alien]
    #2171603 - 12/11/03 10:48 PM (17 years, 2 months ago)

Alien, the quinoa jars colonized good and fast, but like most of other jars they were overincubated and they didn?t fruit.
If I?m ever going to repeat this test, I?ll be sure to include the quinoa again.


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Re: Multi Substrate Test [Re: Anno]
    #2183712 - 12/16/03 09:25 PM (17 years, 2 months ago)

Quote:

Anno said:
Alien, the quinoa jars colonized good and fast, but like most of other jars they  were overincubated and they didn?t fruit.
If I?m ever going to repeat this test, I?ll be sure to include the quinoa again.




Does overincubating cause problems like few to no fruit?  That may answer a problem a foaf had!  :wink:

Peace,
-TekNut-


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Re: Multi Substrate Test [Re: TekNut]
    #2184086 - 12/17/03 12:04 AM (17 years, 2 months ago)

You should still be able to get it to fruit, unless it's so old it's turning mushy or yellow because of an excess of waste products.

Usually if you overincubate and there is even a small amount of light your stuff will pin. It's good for a pretty long time, but if it's fully colonized for more than a month it may be dying....

--
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Re: Multi Substrate Test [Re: micro]
    #2185675 - 12/17/03 06:00 PM (17 years, 2 months ago)

Indeed. I was able to transfer old mycelium but not fruit it.


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OfflineJameZTheNewbie
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Re: Multi Substrate Test [Re: Anno]
    #2188696 - 12/18/03 09:58 PM (17 years, 2 months ago)

has anyone tried a combination of these substrates?


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Offlinemycophage
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Re: Multi Substrate Test [Re: Anno]
    #2196641 - 12/22/03 10:54 PM (17 years, 2 months ago)

I realize that this thread is getting a bit lengthy, but I saw this on pubmed.
(http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?db=PubMed)
Query: psilocybin. Interesting that they looked at cap/stem potency differences.

------------------------------------------------------
Morphological and chemical analysis of magic mushrooms in Japan.

Tsujikawa K, Kanamori T, Iwata Y, Ohmae Y, Sugita R, Inoue H, Kishi T.

National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, 277-0882, Chiba, Japan

Morphological and toxicological analyses were performed on hallucinogenic mushrooms that are currently circulated in Japan. Scanning electron microscope (SEM) indicated a three-dimensional microstructures in the mushrooms. The complementary use of SEM with an optical microscope was effective for observing characteristic tissues, such as basidiomycetes, spores, cystidia and basidia. Hallucinogenic alkaloids were extracted with methanol and determined by high performance liquid chromatography (HPLC) with a UV detector set at 220nm. The psilocin/psilocybin contents in Psilocybe cubensis were in the range of 0.14-0.42%/0.37-1.30% in the whole mushroom (0.17-0.78%/0.44-1.35% in the cap and 0.09-0.30%/0.05-1.27% in the stem), respectively. The hallucinogenic alkaloids in Copelandia were 0.43-0.76%/0.08-0.22% in the whole mushroom (0.64-0.74%/0.02-0.22% in the cap and 0.31-0.78%/0.01-0.39% in the stem). It thus appears that P. cubensis is psilocybin-rich, whereas Copelandia is psilocin-rich.


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InvisibleTHEBOSS
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Re: Multi Substrate Test [Re: mycophage]
    #2299656 - 02/04/04 06:35 AM (17 years, 1 month ago)

Bump..................


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OfflineAnnoA
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Re: Multi Substrate Test [Re: THEBOSS]
    #2299667 - 02/04/04 06:50 AM (17 years, 1 month ago)

No need to bump this really.

"Only a couple of casings fruited. So I won?t be able to use this batch for much.
I will repeat the experiment at a later time. "


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Invisibleutopianglory
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Re: Multi Substrate Test [Re: Anno]
    #2302325 - 02/04/04 09:17 PM (17 years, 1 month ago)

Where abouts are you in the process Anno?

I just received a shipment of yet another 4kgs of Whey Protein as per my extensive consumption requirements so I would be prepared to test it. Unfortunately though whey protein is traditionally distributed with a: sugars, or b: artificial sweeteners. I have used pure whey (isolate) before though, atleast it was unflavoured but still had approximately 94% purity, which probably means that something artifical was added to preserve it.

Of course it is likely that after a few days the mycellium would win the battle.

Also, whey is an interesting substance in that there are many grades of it, for instance you can obtain massive quantities of lower purity whey which is considered "not fit for human consumption".

Anyway, any ideas on how much should be supplemented per say.. 1/2 pint? I am not sure about using my current protein, it is fairly supplemented by sweeteners and whatnot. I may obtain some unflavouted pure stuff. I would imagine storing the protein in the fridge for a few days ought to break down the sugars though.


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OfflineAnnoA
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Re: Multi Substrate Test [Re: utopianglory]
    #2303622 - 02/05/04 03:55 AM (17 years, 1 month ago)

>Where abouts are you in the process Anno?

No where, as I said, I will repeat the experiment at a later time. This time hasn?t come yet.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Substrates

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