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 Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 1 day, 10 hours
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 Re: Multi Substrate Test  [Re: Phoogle]
#1996080 - 10/10/03 02:25 AM (20 years, 2 months ago) |
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Not all casings are producing fruits, yet.
This can be a problem, since you can?t compare the different substrates if you don?t have any fruits from those substrates.
Let?s give it some time and see what happens.
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lysergic
Mycophile!
Registered: 06/09/03
Posts: 691
Last seen: 20 years, 10 days
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Re: Multi Substrate Test [Re: Anno]
#1996125 - 10/10/03 03:23 AM (20 years, 2 months ago) |
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Possibly this test could demonstrate that extended periods of cold /suspended animation lead to lower numbers of pins ? As long as we learn something, I believe it's a sucess.
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In response to an attack killing 15 American Servicemen
PsiloKitten said:
Just give em a little more time, the iraqis are making great progress. And this is unorganized. Wait till they get organized.
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 Zen Peddler
Registered: 06/18/01
Posts: 6,379
Loc: orbit
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Re: Multi Substrate Test [Re: lysergic]
#1996600 - 10/10/03 09:40 AM (20 years, 2 months ago) |
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Not really - one test is never conclusive - especially on that type of hypothesis. At the end of the day, it will only demonstrate what many people already know - millet is a better substrate than rye/brown rice and most others...
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 1 day, 10 hours
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Re: Multi Substrate Test [Re: Zen Peddler]
#1996756 - 10/10/03 10:37 AM (20 years, 2 months ago) |
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>At the end of the day, it will only demonstrate what many people
>already know - millet is a better substrate than rye/brown rice and
>most others...
Hehe, why don?t you wait till the outcome? 
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Flux
Symmetry Breaker
Registered: 06/04/03
Posts: 309
Loc: The Shroomery... retard
Last seen: 18 years, 1 month
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 Re: Multi Substrate Test [Re: Anno]
#1996965 - 10/10/03 11:51 AM (20 years, 2 months ago) |
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I've been quite interested in conducting a similar experiment myself only using various mixtures of different nutritional ingredients in the same jar. I wonder the outcome of mixing WBS, BRF, verm, corn, etc.. in sensible combinations and ratios (not necessarily all of those in the same jar) to determine what variety of nutrients in the correct proportions will produce faster/better more powerful flushes.
Instead of water I'm consider using nutritional (beef, chicken) broths in leu of or addition to just plain water. I wonder if a diluted agar solution would bring about any significant growth affects. What do you think? Agar Tek?
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What'd I tell you about looking at my signature bitch!
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JazzMatazz
addict
Registered: 09/07/02
Posts: 770
Loc: Vienna, Austria
Last seen: 2 years, 4 months
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Re: Multi Substrate Test [Re: Flux]
#1998179 - 10/10/03 05:07 PM (20 years, 2 months ago) |
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well agar is just for making a small piece o mycleium and tzhen spawninh some substrae. I owuld think agar spawns quicker than a syringe , but that's no suprise.Casue you've already got mycelium, whch a syringe doesnt have.
Jazz (drunk)
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Perception is limited to consciousness.Expand it and unfold other realities.
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Flux
Symmetry Breaker
Registered: 06/04/03
Posts: 309
Loc: The Shroomery... retard
Last seen: 18 years, 1 month
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 Re: Multi Substrate Test [Re: JazzMatazz]
#1999871 - 10/11/03 10:38 AM (20 years, 2 months ago) |
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Actually I'm thinking about adding those canned chicken broths to my jars because a.) they have already bean sterilized, b.) having already been sterilized and skimmed so I don't have to worry about my substrate thickening/sticking from protein coagulation. Also, since fungi are chemoheterotrophs I could image at least some benefits from adding extra amino acids.  mmmm... vagina
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What'd I tell you about looking at my signature bitch!
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Tremor1127
Mental Member
Registered: 08/10/03
Posts: 3,404
Loc: In a Van Down By the Rive...
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Post deleted by Administrator [Re: Anno]
#2024081 - 10/19/03 10:31 PM (20 years, 1 month ago) |
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TekNut
********
Registered: 01/01/03
Posts: 382
Loc: TX Gulf Coast
Last seen: 13 years, 6 months
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Re: Multi Substrate Test [Re: Tremor1127]
#2028778 - 10/21/03 12:54 PM (20 years, 1 month ago) |
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Anything new happening here? We should be getting close to some new photos or something.
-TekNut-
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deanofmean
mycophagous
Registered: 12/06/02
Posts: 2,017
Loc: PNW
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Re: Multi Substrate Test [Re: TekNut]
#2028881 - 10/21/03 01:26 PM (20 years, 1 month ago) |
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i think Anno may be running another experiment, to see how long it takes to ware down people's patients . 
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 1 day, 10 hours
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Re: Multi Substrate Test [Re: deanofmean]
#2031390 - 10/22/03 01:56 AM (20 years, 1 month ago) |
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Lol, no, that?s not the case, but in the moment there isn?t much to report.
I harvested a couple of the casings, and the rest are either slowly beginning to pin, or they do nothing.
As I said, if I don?t get enough fruits from the "crucial" substrates, I will repeat the experiment at a later moment when I?m at home for a longer period of time.
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Tremor1127
Mental Member
Registered: 08/10/03
Posts: 3,404
Loc: In a Van Down By the Rive...
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Post deleted by Administrator [Re: Anno]
#2031585 - 10/22/03 04:14 AM (20 years, 1 month ago) |
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daussaulit
Forgetful
Registered: 08/06/02
Posts: 2,894
Loc: Earth
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Re: Multi Substrate Test [Re: Tremor1127]
#2075398 - 11/05/03 10:46 PM (20 years, 1 month ago) |
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bump. what is the status anno?
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 1 day, 10 hours
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Re: Multi Substrate Test [Re: daussaulit]
#2075685 - 11/06/03 12:06 AM (20 years, 1 month ago) |
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Only a couple of casings fruited. So I won?t be able to use this batch for much.
I will repeat the experiment on a later time.
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Paid
Pict
Registered: 03/12/03
Posts: 5,376
Loc: Zone ate
Last seen: 19 years, 9 months
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Re: Multi Substrate Test [Re: Anno]
#2075880 - 11/06/03 01:08 AM (20 years, 1 month ago) |
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Looking forward to it :]
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Told
Artiest
Registered: 03/09/03
Posts: 343
Loc: Rockies
Last seen: 19 years, 3 months
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Re: Multi Substrate Test [Re: Paid]
#2160530 - 12/05/03 03:34 AM (20 years, 7 days ago) |
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no pics  im sad now
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 1 day, 10 hours
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Re: Multi Substrate Test [Re: Told]
#2160732 - 12/05/03 07:32 AM (20 years, 7 days ago) |
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No pictures.
What I did harvest recently is a GT clone on birdseed and rye, and a B+ clone on birdseed and rye.
I will sent those in, let?s see if the tests can be made with them.
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back2growing
hunter
Registered: 06/09/03
Posts: 165
Loc: southeast usa
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Re: Multi Substrate Test [Re: Anno]
#2166247 - 12/07/03 10:40 AM (20 years, 5 days ago) |
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this place that your sending your fruits to be tested, is this open to the public? i would really like to send in some wild cubes here from louisiana just to get an idea of their potency compared to homegrown fruits.
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Its cube season on the gulf coast.
Whats more natural than a man going out and collecting medicines?
I like to take the heavenly route to hell.
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 1 day, 10 hours
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Open to public...well, it depends, it?s the Mycology Department of the National Museum in Prag, Czech Republic.
I know a person there who will try to get this thing organized.
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micro
bunbun has a gungun
Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Multi Substrate Test [Re: Anno]
#2167205 - 12/07/03 05:56 PM (20 years, 5 days ago) |
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If you have access to a spectrophotometer, all you need to do is extract w/ acetic acid/ether from the fruitbodies -- the analogues should be in a sufficient quantity to test the peaks on psilocybin in ethanol. I think the best is at like 256 nm, but I'd have to check. I know it's listed in Merck.
This would get you somewhat of a quantitative result -- probably enough if one were to have enough samples to provide a reasonable mean.
There's always TLC/NMR or HPLC, too....
--
Micro
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Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)
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