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OfflineAnnoA
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Re: Multi Substrate Test [Re: TekNut]
    #1734574 - 07/21/03 12:31 PM (17 years, 7 months ago)

I?m anxious to see the results too.
Yesterday I learned it?s not a good idea to try to make a coherent post at 3AM. :wink:

Not only I included 2 of the corn jars, but I forgot to include the "finch birdseed" and the "amaranth" jar in the photo. 


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InvisibleAlien
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Post deleted by Administrator [Re: Anno]
    #1734804 - 07/21/03 02:00 PM (17 years, 7 months ago)



--------------------
-Alien :cool:

Alien Substrate Instructional:  http://www.youtube.com/watch?v=KG82xLzrNg8


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Offlinecupid
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Re: Multi Substrate Test [Re: Alien]
    #1735093 - 07/21/03 03:46 PM (17 years, 7 months ago)

I don't know, althought ANNO has proven time and time again to be VERY reliable with varying studies (and besides i could get flamed) . I do not agree that this will end the classic which substrate is better argument, there are WAY TOO MANY variables, an example could be the humidity inside the terrarium affecting different substrates and their absorbtion rates ? also guaging growth on a time sacal might not be accurate, what if rice is faster to yeild say 10 grams and WBS is lower to yeild only 7 grams , on the first flush and 7 more on the second ? which then is better ?
Therefore I do not believe that any ""professional"" grower could make a decision based on this thread.

P.S. i still think its cool though


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Anonymous

Re: Multi Substrate Test [Re: cupid]
    #1735112 - 07/21/03 03:51 PM (17 years, 7 months ago)

Alien... the quinoas man!


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Invisibledeanofmean
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Re: Multi Substrate Test [Re: Alien]
    #1735838 - 07/21/03 07:10 PM (17 years, 7 months ago)

Good work Anno . The lentils look awsome :thumbup:

Quote:

Alien said:
:thumbup:  :thumbup:  :thumbup:

I wish one with quinoa could have been done. 




I was thinking the same thing  :tongue: and what about rye grass seed ?
Also, i would be interested to see how "The Alien Tec" compared .  :smile: 


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Invisibledog
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Re: Multi Substrate Test [Re: Anno]
    #1735876 - 07/21/03 07:26 PM (17 years, 7 months ago)

Very cool Anno. My friend will be watching this one.  :thumbup: 


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OfflineNewSpore
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Re: Multi Substrate Test [Re: dog]
    #1735882 - 07/21/03 07:30 PM (17 years, 7 months ago)

bookmarked this one!


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InvisibleHippie3
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Re: Multi Substrate Test [Re: NewSpore]
    #1735931 - 07/21/03 07:47 PM (17 years, 7 months ago)

cupid, it's very true that there are many uncontrolled variables here that preclude this from truly being 'scientific'.
nevertheless the info, incomplete as it will be, should still expand our knowledge and perhaps point in some directions for further study.


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OfflineRaadt
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Re: Multi Substrate Test [Re: Anno]
    #1736085 - 07/21/03 08:42 PM (17 years, 7 months ago)

The Annovator at it again. This is very excellent. Thanks so much for being the 1st one to actually complete the experiment that so many have desired. I cannot wait to see the results of the chromatography. That will be truly interesting.


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-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Offlinecupid
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Re: Multi Substrate Test [Re: Hippie3]
    #1736089 - 07/21/03 08:44 PM (17 years, 7 months ago)

is it not immediatley evident that the substrate with the simplest sugar groups and most basic acids and peptones will show signs of growth before others because they would be the easiest / quickest for the mycelium to ""digest"". A real study IMHO would be to create agar plates with different peptone/acid/protein combos in them and to test them for viability, a PERFECT source of these compounds as ANY SERIOUS shroomerite would already know is in protien powder meal replacment drink mix and many weight gainer products.

To specify these products in specific SOY PROTEIN powder would in theory be like major STREOIDS for mycelium, just be sure not to use whey power as it is a milk product.

PM with any more questions


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OfflineRaadt
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Re: Multi Substrate Test [Re: cupid]
    #1736095 - 07/21/03 08:47 PM (17 years, 7 months ago)

Experiments have been done on agar formulas ;\

you can read TMC if you'd like to see the results. I believe Anno has done some here as well. It still shows no results for pinning, thorough nutrients for all cycles of life, and chromatography results. You need fruitbodies for these experiments, and that is what anno is testing.


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Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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OfflineRaadt
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Re: Multi Substrate Test [Re: Cow Shit Collector]
    #1736101 - 07/21/03 08:49 PM (17 years, 7 months ago)

Quote:

Cow Shit Collector said:
Finally decided to put the old 'which substrate is the best' to rest? :smile:
Next we'll have to test strains for the highest concentrations!  :mushroom2:

*Claps*  Very good experiment Anno.  This one has been long needed.  Thank you! 




Quote:

Anno said:
After the harvest I will have a the mushrooms from the different substrates tested for psilocybin and psilocin with a liquid chromatography.




:confused:  :confused:  :confused:  :confused:

Did he not say that was going to happen? heh.


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Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Offlinecupid
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Re: Multi Substrate Test [Re: Raadt]
    #1736118 - 07/21/03 08:56 PM (17 years, 7 months ago)

perhaps you did not fully understand the scope of the study I have proposed here. In TMC it states that the principal growth chemicals needed for mushrooms vary greatly from chlorophyll producing plants, these chemicals are to my understanding called peptodes and if introduced to a culture should have much the same effect as adding Nitrogen and other fertilizers to Green plants, like miracle grow for mushrooms. We should then through the methods you have listed conduct a study into which OTC product contains the ""best mix"" for our our specific purposes.
As for the testing of fruitbodies I was misled by one statment saying after X amount of days there is this much growth showun here but not over there, which led ME to conclude that this was a race against time study.


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OfflineRaadt
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Re: Multi Substrate Test [Re: cupid]
    #1736156 - 07/21/03 09:20 PM (17 years, 7 months ago)

It's a comprehensive study. Hence his mentioning of the chromatography. Of course there are variables, and most likely no home cultivator will ever be able to construct an environment which will be a truly perfect "control", for these variables. But in the meanwhile what he's doing is very good.

I'm not sure one could find the "perfect additive mix" without knowing exactly what each specie/race/isolate is breaking down and at what rate.

Right now he is trying to find -- albeit roughly, the best substrate for people to use in home cultivation via. the PF tek.


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Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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InvisibleHippie3
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Re: Multi Substrate Test [Re: Raadt]
    #1736230 - 07/21/03 09:54 PM (17 years, 7 months ago)

exactly, there could never be a truly 'perfect' substrate unless we all were growing the exact same genetic substrain, otherwise minor differences would make one substrain prefer rice, like pfc's did, while others prefer horse dung, etc.


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InvisibleAlien
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Post deleted by Administrator [Re: Hippie3]
    #1736929 - 07/22/03 02:03 AM (17 years, 7 months ago)



--------------------
-Alien :cool:

Alien Substrate Instructional:  http://www.youtube.com/watch?v=KG82xLzrNg8


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OfflineAnnoA
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Re: Multi Substrate Test [Re: Alien] * 1
    #1737180 - 07/22/03 03:41 AM (17 years, 7 months ago)

>I would like a rundown on how the liquid chromatography will be done; IE a lab or home?

A HPLC analysis in a lab in Prague.
I can include quinoa too, sure, and rye grass seeds.

As for the argument of too many variables etc, etc....
This will always be the case, with every experiment you do, regardless of the subject tested.
But one has to start somewhere, talking about doing the "perfect" experiment is good, but doing an imperfect one is still better, IMO.


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InvisibleAlien
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Post deleted by Administrator [Re: Anno]
    #1737372 - 07/22/03 05:43 AM (17 years, 7 months ago)



--------------------
-Alien :cool:

Alien Substrate Instructional:  http://www.youtube.com/watch?v=KG82xLzrNg8


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Offlineblackout
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Re: Multi Substrate Test [Re: Alien]
    #1737443 - 07/22/03 07:00 AM (17 years, 7 months ago)

best experiment in ages. how are you planning on testing them e.g. just picking a simiar size and maturity fruitbody from each? or harvest the entire batch, dry it and make a homogenous mix to get an average?

it would also be interesting to see the yeild from each too and time taken to finish. also differences from 1st and 2nd flush. just like cannabis strains, some give huge yield but a lower weight producing strain may give more THC per watt. i dont mind the taste of shrooms too much so i would go with the one which gives the most psilocybin in the shortest time with the cheapest grain, this doesnt necessarily mean they are potent.

white rice Vs brown would be interesting. another i thought of is brewers yeast, using it just like BRF with verm

as for cat food, 9 out of 10 cubensis prefer whiskas ;-)


Edited by blackout (07/22/03 07:03 AM)


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OfflineAnnoA
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Re: Multi Substrate Test [Re: blackout]
    #1737573 - 07/22/03 08:52 AM (17 years, 7 months ago)

> how are you planning on testing them e.g. just picking a simiar size
>and maturity fruitbody from each? or harvest the entire batch, dry it
>and make a homogenous mix to get an average?

The later, harvesting them when the veil begins to tear. I?d like to obtain average values for each substrate.
I thought about combining the first 2 flushes.

I added 3 more substrates to the experiment today: quinoa, rye grass seed and spelt.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Substrates

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