Multi Substrate Test - failed - Substrates

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation > Mushroom Cultivation Archive > Substrates

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Anno
Experimenter

Registered: 06/17/99
Posts: 24,157
Loc: my room
Last seen: 19 days, 8 hours

Multi Substrate Test - failed

#1732981 - 07/20/03 05:55 PM (19 years, 10 months ago)

Update:
Only a couple of casings fruited. So I won?t be able to use this batch for much.
I will repeat the experiment at a later time.

After much debate in this thread I decided to try and obtain some data on which substrate produces the most potent mushrooms.
I prepared several jars for each substrate according to the PF tek(actually according to PF-Tek for Simple Minds ), pressure cooked for 1 hour and inoculated with liquid mycelium prepared according to the Mycelium Inoculation Technique

The dung jar and the straw jar were prepared just with pure substrate brought to the correct moisture before sterilizing.
The potato jar was prepared with grated potatoes and around 20% moist vermiculite by volume.

The jars were inoculated on the 16th and the 17th this month with the Amazon strain isolate already tested for good pinning characteristics.

For now all the substrates show growth, except for the catfood. The growth in the dung and in the straw jar is very wispy, but is spread over quite some area. Overall the BRF jars show the fastest growth.

After the harvest I will have a the mushrooms from the different substrates tested for psilocybin and psilocin with a liquid chromatography.

PS: the observing reader will notice that there are 2 "corn" jars in the picture, I put the second there by mistake, and didn?t feel like making the shot again after I noticed it, because I placed the jars already back into the incubator.

Edited by Anno (02/10/04 07:37 AM)

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