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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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OfflineAnnoA
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Multi Substrate Test - failed
    #1732981 - 07/20/03 07:55 PM (13 years, 7 months ago)

Update:
Only a couple of casings fruited. So I won?t be able to use this batch for much.
I will repeat the experiment at a later time.



After much debate in this thread I decided to try and obtain some data on which substrate produces the most potent mushrooms.
I prepared several jars for each substrate according to the PF tek(actually according to PF-Tek for Simple Minds ), pressure cooked for 1 hour and inoculated with liquid mycelium prepared according to the Mycelium Inoculation Technique

The dung jar and the straw jar were prepared just with pure substrate brought to the correct moisture before sterilizing.
The potato jar was prepared with grated potatoes and around 20% moist vermiculite by volume.



The jars were inoculated on the 16th and the 17th this month with the Amazon strain isolate already tested for good pinning characteristics.

For now all the substrates show growth, except for the catfood. The growth in the dung and in the straw jar is very wispy, but is spread over quite some area. Overall the BRF jars show the fastest growth.


After the harvest I will have a the mushrooms from the different substrates tested for psilocybin and psilocin with a liquid chromatography.

PS: the observing reader will notice that there are 2 "corn" jars in the picture, I put the second there by mistake, and didn?t feel like making the shot again after I noticed it, because I placed the jars already back into the incubator.


Edited by Anno (02/10/04 09:37 AM)


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Offlinerealrasta
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Re: Multi Substrate Test [Re: Anno]
    #1733081 - 07/20/03 08:46 PM (13 years, 7 months ago)

very good anno i love to see this stuff. great job. this is very intresting. just like all your post thanks so much for your hard work keep it up its great


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InvisibleJared
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Re: Multi Substrate Test [Re: realrasta]
    #1733125 - 07/20/03 09:14 PM (13 years, 7 months ago)

Excellent experiment :smile:


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Offlinejoeinc
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Re: Multi Substrate Test [Re: realrasta]
    #1733126 - 07/20/03 09:15 PM (13 years, 7 months ago)

Cool... I've been contemplating replacing the brf in PF tek with cornmeal to see what happens...with all this hype abount popcorn I'd like to compare a straight pf cake to a cornmeal cake....I've done some preliminary experiments with the cornmeal and find its not very water soluable though... I'm wondering because when i make cakes the brf doesnt generally totally dissolve.. My question to you, Is it better to have a water soluable, or a somewhat water resistant nutrient base? I'm only asking because you seem to be very well informed :P


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OfflineFallenShroom
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Re: Multi Substrate Test [Re: joeinc]
    #1733168 - 07/20/03 09:44 PM (13 years, 7 months ago)

Very cool can't wait to see the hypothesis of this experiment.


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OfflinePsilocybin_monkey
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Re: Multi Substrate Test [Re: FallenShroom]
    #1733182 - 07/20/03 09:50 PM (13 years, 7 months ago)

hahah thats funny that they have cat food in there too


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OfflineSnobrdr311
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Re: Multi Substrate Test [Re: Psilocybin_monkey]
    #1733453 - 07/21/03 12:03 AM (13 years, 7 months ago)

This is a great experiment, thank you for doing it.


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InvisibleFiend_13
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Re: Multi Substrate Test [Re: Snobrdr311]
    #1733628 - 07/21/03 01:02 AM (13 years, 7 months ago)

Dont look like Good Ol catfood isint workin to well?


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InvisibleJared
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Re: Multi Substrate Test [Re: Fiend_13]
    #1733711 - 07/21/03 01:38 AM (13 years, 7 months ago)

What brand and kind of catfood is it..? I have a feeling the different kinds could make a difference, since they're all made from different source substrates..


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OfflinePsilocybin_monkey
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Re: Multi Substrate Test [Re: Jared]
    #1733717 - 07/21/03 01:43 AM (13 years, 7 months ago)

that is a good point jared maybe if you tried a different brand of cat food you may have got better results. hahahah (j/k)


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InvisibleMrMaddHatter
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Re: Multi Substrate Test [Re: Anno]
    #1733828 - 07/21/03 02:29 AM (13 years, 7 months ago)

WOW :smile:
Can't wait for the results! 


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OfflineAnnoA
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Re: Multi Substrate Test [Re: Jared]
    #1733868 - 07/21/03 02:48 AM (13 years, 7 months ago)

>What brand and kind of catfood is it..?

It?s Brekkies 3 Variations dry food (Beef, Chicken and Vegetables)


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InvisibleCow Shit Collector
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Re: Multi Substrate Test [Re: Anno]
    #1733986 - 07/21/03 03:43 AM (13 years, 7 months ago)

Finally decided to put the old 'which substrate is the best' to rest? :smile:
Next we'll have to test strains for the highest concentrations!  :mushroom2:

*Claps*  Very good experiment Anno.  This one has been long needed.  Thank you!


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InvisibleHippie3
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Re: Multi Substrate Test [Re: Cow Shit Collector]
    #1734391 - 07/21/03 10:15 AM (13 years, 7 months ago)

looking forward to more on this one, anno.


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Re: Multi Substrate Test [Re: Anno]
    #1734424 - 07/21/03 10:58 AM (13 years, 7 months ago)

Very cool experiment Anno, the results should be interesting.


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Re: Multi Substrate Test [Re: Effed]
    #1734451 - 07/21/03 11:18 AM (13 years, 7 months ago)

hey anno,
you should add a jar of hominy.


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Anonymous

Re: Multi Substrate Test [Re: Hippie3]
    #1734469 - 07/21/03 11:25 AM (13 years, 7 months ago)

:thumbup: *adds to favorites*


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OfflineAnnoA
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Re: Multi Substrate Test [Re: Hippie3]
    #1734487 - 07/21/03 11:39 AM (13 years, 7 months ago)

>you should add a jar of hominy.

Do you think there is a major difference to corn ?


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Re: Multi Substrate Test [Re: Anno]
    #1734500 - 07/21/03 11:49 AM (13 years, 7 months ago)

hominy has the hulls removed so yeah, it's a bit different.
you can use it right out of the can and it will colonize.


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OfflineTekNut
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Re: Multi Substrate Test [Re: Anno]
    #1734562 - 07/21/03 12:23 PM (13 years, 7 months ago)

Very cool experiment Anno.  It'll be nice to see how your tests turns out.  And it also looks as if you've pretty much covered the whole range of substrates pretty well.  This thread is getting marked as a favorite!  :smile:

Peace and keep up the good work!
-TekNut- 


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Substrates

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